Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,133	T→G	S146S (TCT→TCG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,133	0	T	G	92.9%	124.9 / ‑1.9	42	S146S (TCT→TCG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (28/11);  total (30/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

GGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCG  >  NC_000913/4636902‑4637371                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ggCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGcgc                                                                                                                                                                                                                             >  1:286564/1‑249 (MQ=255)   cGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGCGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCg                                                                                                                                                                                                                           >  1:322413/1‑251 (MQ=255)        aaTCAGCTACAGCTACCCCCGTTTCGCGCCAAAAGCGGGGTCATTAACATAGTGCGCAAGGAATAGTAAGTCTATATGACCGTTTCGCAGGGTAAAGTATTGTGCGATTCGGCAAATAAAGCCGTTGGGCAGTATTTGTCGCGCTGGTATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGGTCTTTGCAAAATCCTGCCGATTCCGAAAGTTCGGTGATGTATGTTGCCTCGCCGATTAt                                                                                                                                                                                                                      <  2:75929/251‑1 (MQ=255)               tACAAAATCGACAGTTTAGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTAGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTTGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGAGTGGTCAGTATGGTGAGAGGAGCACGTTGCAAAATCCTGCCGATCCCGAATGGTGGGTGATGTATGttacctagcggattttggacggg                                                                                                                                                                                                               >  2:173737/1‑230 (MQ=255)                         cccGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAACGAATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGACTGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCtt                                                                                                                                                                                                      >  1:179017/1‑250 (MQ=255)                            gTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTg                                                                                                                                                                                                  >  1:310104/1‑251 (MQ=255)                                      aaTATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCGAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTg                                                                                                                                                                                          >  1:193692/1‑249 (MQ=255)                                                 cATTAACAAAGAGCGCAATGAATATCATGTATATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGATCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAAc                                                                                                                                                                             >  2:68967/1‑251 (MQ=255)                                                           gTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACgcggc                                                                                                                                                                   >  1:367142/1‑251 (MQ=255)                                                            tGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCAGCGCCGATTATGGACGGCTCGAGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACgcgggg                                                                                                                                                                  >  2:315619/1‑249 (MQ=255)                                                                    gAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTcc                                                                                                                                                          <  1:315619/251‑1 (MQ=255)                                                                      atatCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGGTTTGAGCGTAGGCAAACCGACCGCGGCGATGGCTCCgg                                                                                                                                                        >  1:71407/1‑251 (MQ=255)                                                                               cTATATGACAGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCTCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAACGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTCTTGGCGGTTTGTACGTCGGCAAAACGAACGCGGAGATGGCTCCGGTCAttgatc                                                                                                                                               >  2:152/1‑247 (MQ=255)                                                                                 atatGACCGATTCGCAGGGTAAAGTATTTTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTTGGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATGCCGAAAGTACGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGGTTATGGGCGTTGTGAGCGTAGGCAAACCGATCGCGGCGATGGCTCCGGTCATTAAGCGt                                                                                                                                             <  1:56891/251‑1 (MQ=255)                                                                                  tatGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGTCAGGATTATGCGCGGTGGAATGACGTATGTCTAACTTAGCGTGGTCAGAATGGTTCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAACGTTAGGTGATGTTTGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGCCAAACAGAAGGCGGCGagatctcagttcagtagacgt                                                                                                                                             >  2:120509/1‑230 (MQ=255)                                                                                        ggcttcgcaggggatattATTTTTCGATTCGGAAAATAAAGCCGTTTGGCTGGATTATGCGCGTGGGAATGACTTCTGGCTAACGTTGCGGGGTCAGTTTGGTGCGCGCAGCTCGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTGTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTagcgagc                                                                                                                                      <  2:16459/234‑1 (MQ=255)                                                                                                aGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAAtt                                                                                                                              <  1:47743/251‑1 (MQ=255)                                                                                                  ggtaaaGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAAtttt                                                                                                                            >  1:34605/4‑251 (MQ=255)                                                                                                    cAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTAt                                                                                                                          >  1:9806/1‑251 (MQ=255)                                                                                                          aTTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGcc                                                                                                                     >  1:157051/1‑250 (MQ=255)                                                                                                            tGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAgc                                                                                                                  >  1:363832/1‑251 (MQ=255)                                                                                                             gTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATGCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAgc                                                                                                                  <  1:87250/250‑1 (MQ=255)                                                                                                                 gATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCAt                                                                                                             >  1:354692/1‑251 (MQ=255)                                                                                                                             aaaGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGAt                                                                                                 >  1:167497/1‑251 (MQ=255)                                                                                                                               agcatttGGTCAGGAATATTCGCACTAGATTGACGTCGGGCAAGCGTTGCGGGGTCGGTATGGTGCGTGCTGTATGTTGCAAAATCCTGCCGATCCCGAAAGTCCGGTGATGTATTTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGGTTTGAGCGTAGGCAAACCGATCGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGACTTTTATGGGCCAGCGCCATTTTGCTGGGGATTg                                                                                               <  2:58162/246‑1 (MQ=255)                                                                                                                               aGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTg                                                                                               >  1:9928/1‑251 (MQ=255)                                                                                                                                 cggTTGGACAGGATTTTTCGGGCTGGAATGAGGTCTGTCTAACGTTGCGTGGTCGGTATGGTGGGCGCAGGTCGTTGCAAAATCCTGCCGATCCCGAATGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGGTTATTGGCGTTTTGAGCGTAGGCAAACCGAATGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGCGCCAGCGCCATTTTGTTGGGGATTGCa                                                                                             <  2:193692/249‑1 (MQ=255)                                                                                                                                 ccGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCa                                                                                             <  1:249729/251‑1 (MQ=255)                                                                                                                                   gTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACt                                                                                           >  1:265707/1‑251 (MQ=255)                                                                                                                                     tgggcAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTgg                                                                                         >  1:285102/5‑251 (MQ=255)                                                                                                                                        gcAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTg                                                                                       >  1:242057/2‑250 (MQ=255)                                                                                                                                        gcAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTg                                                                                       >  1:214204/2‑250 (MQ=255)                                                                                                                                                 ttCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGgcgc                                                                               >  1:131645/1‑249 (MQ=255)                                                                                                                                                                cGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTtggtgg                                                               >  1:40187/1‑250 (MQ=255)                                                                                                                                                                         aaCGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGct                                                     >  1:20069/1‑251 (MQ=255)                                                                                                                                                                            gTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGctcc                                                   >  1:345158/1‑249 (MQ=255)                                                                                                                                                                                      cattATGGTGCTCGCAGTACGTTGCAAAATCCTGCCTATCCCGAAAGTTCGGTTATGTATGTTGCCGCGCCGATTATTGACGGCTCGCGGCTTTTTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCt                                        <  1:302827/248‑1 (MQ=255)                                                                                                                                                                                                            tGCAAAATCCTGCCGATCCCGAAAGATCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTc                  >  1:299609/1‑251 (MQ=255)                                                                                                                                                                                                               aaaaTCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACt               <  1:179178/251‑1 (MQ=255)                                                                                                                                                                                                                 aaTCCTGCCGATCCCGAAAGGTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTTGTGGATCAACCGCTCCATTGCCCGGCCCACTCGCCATGCCGATTCCGTCACTg              >  2:67100/1‑250 (MQ=255)                                                                                                                                                                                                                         cGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGcc                                             >  1:168389/1‑211 (MQ=255)                                                                                                                                                                                                                             cccGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCg  <  1:171011/250‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCG  >  NC_000913/4636902‑4637371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑