Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,037	A→G	G114G (GGA→GGG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,037	0	A	G	93.0%	109.7 / ‑1.9	45	G114G (GGA→GGG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (24/16);  total (27/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e-01

GGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACT  >  NC_000913/4636791‑4637282                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      gggCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAgg                                                                                                                                                                                                                                                   >  1:58162/1‑251 (MQ=255)               cAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTgg                                                                                                                                                                                                                                     >  1:52661/1‑251 (MQ=255)                 aCGGAGGGGACGTTAATTGATACCGCAACGGTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCTAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATTGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGaa                                                                                                                                                                                                                                   <  2:279658/251‑1 (MQ=255)                       gggACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCTGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGt                                                                                                                                                                                                                             <  1:121789/251‑1 (MQ=255)                            gttaaTCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGc                                                                                                                                                                                                                        <  1:176522/247‑1 (MQ=255)                                      acCGCAACGTTGCTGGCGTGGCTGGCGCGTCCCGATTTGCGCGCTGGGGACCCATCGCATGGTCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCGCACGTTTCGCGCCAATATCGGTGGCAATAACAAAGTGCGCAATGAACATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTga                                                                                                                                                                                                               <  2:87669/250‑2 (MQ=255)                                         tcAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGCCACAACGCATGGGCCAATGGCGCAGGCGTCTAATCAGCTACAACATCGCCCGTTTCGCGCCCATATCGGTGGCATTAACACAGTTGGCAATGAATAACATGTATATATGACCGCTTCGCAGGGTAAAGTATTGTTCGATTAGGCAAATCAAGCCGTTGGGCAGGATTATTCTAGCTGGAATTCCGTCGGGTTAACGTTGCGTgg                                                                                                                                                                                                           >  2:322398/2‑251 (MQ=255)                                             cGTTGCTGGCGGAGCTGGCGCGCGCCGATTTGATCTCAGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTgtacag                                                                                                                                                                                                       <  2:74948/251‑6 (MQ=255)                                               ttGCTGGCGGAGCTGGCGAGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACACCATCGCACGTTTCGCGCCAAAATCGGTGGCATTAAAAAAGAGCGCAATGAATATCATGTCTATATTACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTAATCGCGCTGGAATGGAGTCTGGCTAACATTGCGTggcaagt                                                                                                                                                                                                      >  2:336111/1‑245 (MQ=255)                                                 gCTGGCGGAGAGGGCGCGTCCCGATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTccacgc                                                                                                                                                                                                    <  1:44427/250‑6 (MQ=255)                                                        gAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTgcgcg                                                                                                                                                                                            >  1:384157/1‑251 (MQ=255)                                                          gCTGGCGCGTCCCGATTTGCTCTCTGGGGCCCCAAAGCCTGGGCAACTGGAGCAGGCGTTTTATCAGCTACAACATAGCCAGTTTCGCGCCAATATCGGTGGAATTAACAAAACGCACAATGACTATCATGTATATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGAAAATAAAGCCGGTGGGCAGGATTATTCGCGCTGGAATGACGTTTAGCTAACGTTGTGTGGTGAATATGCTgcgcga                                                                                                                                                                                           >  2:205040/1‑249 (MQ=255)                                                                     ccGATTTGCTCTCTGGGGACCAAACGCATTGGCCACTGGCGCAGGTGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGTAGGATTATTCGCGCTGGAATGCCGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCaa                                                                                                                                                                               <  1:317816/251‑1 (MQ=255)                                                                          ttGCTCTCTGGGGACCCAACGCATGGTCAACTGGCGCAGGCGTTGAAGCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGTGCAGGATTATTCGCTCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCATCACTTTGCAAAATc                                                                                                                                                                           <  1:44377/250‑1 (MQ=255)                                                                                                                ggCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGcgc                                                                                                                                    >  1:286564/1‑249 (MQ=255)                                                                                                                  cGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGCGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCg                                                                                                                                  >  1:322413/1‑251 (MQ=255)                                                                                                                       aaTCAGCTACAGCTACCCCCGTTTCGCGCCAAAAGCGGGGTCATTAACATAGTGCGCAAGGAATAGTAAGTCTATATGACCGTTTCGCAGGGTAAAGTATTGTGCGATTCGGCAAATAAAGCCGTTGGGCAGTATTTGTCGCGCTGGTATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGGTCTTTGCAAAATCCTGCCGATTCCGAAAGTTCGGTGATGTATGTTGCCTCGCCGATTAt                                                                                                                             <  2:75929/251‑1 (MQ=255)                                                                                                                              tACAAAATCGACAGTTTAGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTAGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTTGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGAGTGGTCAGTATGGTGAGAGGAGCACGTTGCAAAATCCTGCCGATCCCGAATGGTGGGTGATGTATGttacctagcggattttggacggg                                                                                                                      >  2:173737/1‑230 (MQ=255)                                                                                                                                      cGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATg                                                                                                                                                                                                                                 >  1:99087/1‑136 (MQ=255)                                                                                                                                      cGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATg                                                                                                                                                                                                                                 <  2:99087/136‑1 (MQ=255)                                                                                                                                        cccGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAACGAATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGACTGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCtt                                                                                                             >  1:179017/1‑250 (MQ=255)                                                                                                                                           gTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTg                                                                                                         >  1:310104/1‑251 (MQ=255)                                                                                                                                                     aaTATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCGAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTg                                                                                                 >  1:193692/1‑249 (MQ=255)                                                                                                                                                                cATTAACAAAGAGCGCAATGAATATCATGTATATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGATCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAAc                                                                                    >  2:68967/1‑251 (MQ=255)                                                                                                                                                                          gTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACgcggc                                                                          >  1:367142/1‑251 (MQ=255)                                                                                                                                                                           tGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCAGCGCCGATTATGGACGGCTCGAGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACgcgggg                                                                         >  2:315619/1‑249 (MQ=255)                                                                                                                                                                                   gAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTcc                                                                 <  1:315619/251‑1 (MQ=255)                                                                                                                                                                                     atatCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGGTTTGAGCGTAGGCAAACCGACCGCGGCGATGGCTCCgg                                                               >  1:71407/1‑251 (MQ=255)                                                                                                                                                                                              cTATATGACAGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCTCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAACGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTCTTGGCGGTTTGTACGTCGGCAAAACGAACGCGGAGATGGCTCCGGTCAttgatc                                                      >  2:152/1‑247 (MQ=255)                                                                                                                                                                                                atatGACCGATTCGCAGGGTAAAGTATTTTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTTGGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATGCCGAAAGTACGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGGTTATGGGCGTTGTGAGCGTAGGCAAACCGATCGCGGCGATGGCTCCGGTCATTAAGCGt                                                    <  1:56891/251‑1 (MQ=255)                                                                                                                                                                                                 tatGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGTCAGGATTATGCGCGGTGGAATGACGTATGTCTAACTTAGCGTGGTCAGAATGGTTCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAACGTTAGGTGATGTTTGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGCCAAACAGAAGGCGGCGagatctcagttcagtagacgt                                                    >  2:120509/1‑230 (MQ=255)                                                                                                                                                                                                       ggcttcgcaggggatattATTTTTCGATTCGGAAAATAAAGCCGTTTGGCTGGATTATGCGCGTGGGAATGACTTCTGGCTAACGTTGCGGGGTCAGTTTGGTGCGCGCAGCTCGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTGTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTagcgagc                                             <  2:16459/234‑1 (MQ=255)                                                                                                                                                                                                               aGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAAtt                                     <  1:47743/251‑1 (MQ=255)                                                                                                                                                                                                                 ggtaaaGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAAtttt                                   >  1:34605/4‑251 (MQ=255)                                                                                                                                                                                                                   cAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTAt                                 >  1:9806/1‑251 (MQ=255)                                                                                                                                                                                                                         aTTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGcc                            >  1:157051/1‑250 (MQ=255)                                                                                                                                                                                                                           tGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAgc                         >  1:363832/1‑251 (MQ=255)                                                                                                                                                                                                                            gTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATGCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAgc                         <  1:87250/250‑1 (MQ=255)                                                                                                                                                                                                                                gATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCAt                    >  1:354692/1‑251 (MQ=255)                                                                                                                                                                                                                                            aaaGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGAt        >  1:167497/1‑251 (MQ=255)                                                                                                                                                                                                                                              agcatttGGTCAGGAATATTCGCACTAGATTGACGTCGGGCAAGCGTTGCGGGGTCGGTATGGTGCGTGCTGTATGTTGCAAAATCCTGCCGATCCCGAAAGTCCGGTGATGTATTTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGGTTTGAGCGTAGGCAAACCGATCGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGACTTTTATGGGCCAGCGCCATTTTGCTGGGGATTg      <  2:58162/246‑1 (MQ=255)                                                                                                                                                                                                                                              aGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTg      >  1:9928/1‑251 (MQ=255)                                                                                                                                                                                                                                                cggTTGGACAGGATTTTTCGGGCTGGAATGAGGTCTGTCTAACGTTGCGTGGTCGGTATGGTGGGCGCAGGTCGTTGCAAAATCCTGCCGATCCCGAATGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGGTTATTGGCGTTTTGAGCGTAGGCAAACCGAATGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGCGCCAGCGCCATTTTGTTGGGGATTGCa    <  2:193692/249‑1 (MQ=255)                                                                                                                                                                                                                                                ccGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCa    <  1:249729/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACt  >  1:265707/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACT  >  NC_000913/4636791‑4637282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑