| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,636,993 | G→T | A100S (GCG→TCG) | creC → | sensory histidine kinase CreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,636,993 | 0 | G | T | 97.3% | 100.8 / ‑3.5 | 37 | A100S (GCG→TCG) | creC | sensory histidine kinase CreC |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (22/14); total (23/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01 | |||||||||||
CTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGT > NC_000913/4636752‑4637232 | cTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCATTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAgggt > 1:3618/1‑249 (MQ=255) aCTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGt < 1:253636/251‑1 (MQ=255) ctggctctttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGTCGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATTTCGGTGGCCTTACAAAAGTACGCAATGAGAATACGGTCTATATGACCGATTCACAGGGTAAAGTATTGtt > 2:179178/8‑251 (MQ=255) ggctaTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAGCAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGtt < 1:367582/245‑1 (MQ=255) aTTTTTGTCAAAGACGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCACCGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGAATTAACAAAGGGAGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGAATTGTTCGAttt > 2:47743/1‑250 (MQ=255) aaaCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTgg > 1:75929/1‑251 (MQ=255) gggCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAgg > 1:58162/1‑251 (MQ=255) cAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTgg > 1:52661/1‑251 (MQ=255) aCGGAGGGGACGTTAATTGATACCGCAACGGTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCTAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATTGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGaa < 2:279658/251‑1 (MQ=255) gggACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCTGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGt < 1:121789/251‑1 (MQ=255) gttaaTCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGc < 1:176522/247‑1 (MQ=255) acCGCAACGTTGCTGGCGTGGCTGGCGCGTCCCGATTTGCGCGCTGGGGACCCATCGCATGGTCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCGCACGTTTCGCGCCAATATCGGTGGCAATAACAAAGTGCGCAATGAACATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTga < 2:87669/250‑2 (MQ=255) tcAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGCCACAACGCATGGGCCAATGGCGCAGGCGTCTAATCAGCTACAACATCGCCCGTTTCGCGCCCATATCGGTGGCATTAACACAGTTGGCAATGAATAACATGTATATATGACCGCTTCGCAGGGTAAAGTATTGTTCGATTAGGCAAATCAAGCCGTTGGGCAGGATTATTCTAGCTGGAATTCCGTCGGGTTAACGTTGCGTgg > 2:322398/2‑251 (MQ=255) cGTTGCTGGCGGAGCTGGCGCGCGCCGATTTGATCTCAGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTgtacag < 2:74948/251‑6 (MQ=255) ttGCTGGCGGAGCTGGCGAGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACACCATCGCACGTTTCGCGCCAAAATCGGTGGCATTAAAAAAGAGCGCAATGAATATCATGTCTATATTACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTAATCGCGCTGGAATGGAGTCTGGCTAACATTGCGTggcaagt > 2:336111/1‑245 (MQ=255) gCTGGCGGAGAGGGCGCGTCCCGATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTccacgc < 1:44427/250‑6 (MQ=255) gAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTgcgcg > 1:384157/1‑251 (MQ=255) gCTGGCGCGTCCCGATTTGCTCTCTGGGGCCCCAAAGCCTGGGCAACTGGAGCAGGCGTTTTATCAGCTACAACATAGCCAGTTTCGCGCCAATATCGGTGGAATTAACAAAACGCACAATGACTATCATGTATATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGAAAATAAAGCCGGTGGGCAGGATTATTCGCGCTGGAATGACGTTTAGCTAACGTTGTGTGGTGAATATGCTgcgcga > 2:205040/1‑249 (MQ=255) ccGATTTGCTCTCTGGGGACCAAACGCATTGGCCACTGGCGCAGGTGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGTAGGATTATTCGCGCTGGAATGCCGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCaa < 1:317816/251‑1 (MQ=255) ttGCTCTCTGGGGACCCAACGCATGGTCAACTGGCGCAGGCGTTGAAGCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGTGCAGGATTATTCGCTCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCATCACTTTGCAAAATc < 1:44377/250‑1 (MQ=255) ggCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGcgc > 1:286564/1‑249 (MQ=255) cGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGCGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCg > 1:322413/1‑251 (MQ=255) aaTCAGCTACAGCTACCCCCGTTTCGCGCCAAAAGCGGGGTCATTAACATAGTGCGCAAGGAATAGTAAGTCTATATGACCGTTTCGCAGGGTAAAGTATTGTGCGATTCGGCAAATAAAGCCGTTGGGCAGTATTTGTCGCGCTGGTATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGGTCTTTGCAAAATCCTGCCGATTCCGAAAGTTCGGTGATGTATGTTGCCTCGCCGATTAt < 2:75929/251‑1 (MQ=255) tACAAAATCGACAGTTTAGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTAGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTTGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGAGTGGTCAGTATGGTGAGAGGAGCACGTTGCAAAATCCTGCCGATCCCGAATGGTGGGTGATGTATGttacctagcggattttggacggg > 2:173737/1‑230 (MQ=255) cGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATg > 1:99087/1‑136 (MQ=255) cGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATg < 2:99087/136‑1 (MQ=255) cccGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAACGAATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGACTGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCtt > 1:179017/1‑250 (MQ=255) gTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTg > 1:310104/1‑251 (MQ=255) aaTATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCGAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTg > 1:193692/1‑249 (MQ=255) cATTAACAAAGAGCGCAATGAATATCATGTATATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGATCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAAc > 2:68967/1‑251 (MQ=255) gTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACgcggc > 1:367142/1‑251 (MQ=255) tGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCAGCGCCGATTATGGACGGCTCGAGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACgcgggg > 2:315619/1‑249 (MQ=255) gAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTcc < 1:315619/251‑1 (MQ=255) atatCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGGTTTGAGCGTAGGCAAACCGACCGCGGCGATGGCTCCgg > 1:71407/1‑251 (MQ=255) cTATATGACAGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCTCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAACGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTCTTGGCGGTTTGTACGTCGGCAAAACGAACGCGGAGATGGCTCCGGTCAttgatc > 2:152/1‑247 (MQ=255) atatGACCGATTCGCAGGGTAAAGTATTTTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTTGGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATGCCGAAAGTACGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGGTTATGGGCGTTGTGAGCGTAGGCAAACCGATCGCGGCGATGGCTCCGGTCATTAAGCGt < 1:56891/251‑1 (MQ=255) tatGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGTCAGGATTATGCGCGGTGGAATGACGTATGTCTAACTTAGCGTGGTCAGAATGGTTCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAACGTTAGGTGATGTTTGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGCCAAACAGAAGGCGGCGagatctcagttcagtagacgt > 2:120509/1‑230 (MQ=255) | CTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGT > NC_000913/4636752‑4637232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |