Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,201	G→A	L65L (TTG→TTA)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,201	0	G	A	92.3%	98.8 / 0.5	40	L65L (TTG→TTA)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (16/20);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

CGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATC  >  NC_000913/4635957‑4636444                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cgATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTTCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTgccgc                                                                                                                                                                                                                                               >  1:183358/1‑251 (MQ=255) cgATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATgccgc                                                                                                                                                                                                                                               <  1:128164/251‑1 (MQ=255)      aGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCCTGATTCTCGATGTTGGTTTGCCTGCTATCAGCGGCTTTGAATTATGCCGCCGGtt                                                                                                                                                                                                                                          >  2:181600/1‑251 (MQ=255)                     aTGCCGTGGCGGCATAAACAGAGGCGATTTAGGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTTCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATcc                                                                                                                                                                                                                           <  2:183358/251‑1 (MQ=255)                     aTGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATcc                                                                                                                                                                                                                           >  1:108331/1‑251 (MQ=255)                           tggcgcaaaTAACAGAGGCGATTTATGCAACGGGACAAGGCATGGTTAGAGGAAGATGAGCAAGGGATAGACGACACGCTGGTCTCCATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGAGAGGATTGCCGGTGCTGGATAAAGCTCGCAATCAGG‑CACCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGCATTATGCCCACTGTTCCTGGCGCTCCATCCGGCGtt                                                                                                                                                                                                                     >  2:163376/8‑251 (MQ=255)                                          agGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCt                                                                                                                                                                                                      <  1:20254/251‑1 (MQ=255)                                          agGCGATTTATGCAACGGGAAACGGTCTGGATAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCt                                                                                                                                                                                                      >  1:99509/1‑251 (MQ=255)                                                       aacggggaccgttCTGGTTAGTGGAAGATGAGGAAGGGATAGCCGACACGCTGGGCTACATTTTGCAGCAGGAAGGTGTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGt                                                                                                                                                                                         <  2:181463/239‑1 (MQ=255)                                                                tggTCTGGTTAGTGGAAGATGAGAAAGGGATAGCCGACACGCTGGTCTACATGGTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGCGCTTGATAAAGCTCGCCAGCAGG‑CTCCCGACGGCATGATTGTCGATGTTGGTATGCCTGACATCAGCGGCTTTGAATACTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGAACTGTTCCTGACGGCCCGactcgaaggagtc                                                                                                                                                                                >  2:376864/2‑239 (MQ=255)                                                                   gcTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCGGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGAt                                                                                                                                                                             <  1:135023/250‑1 (MQ=255)                                                                       gTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGcc                                                                                                                                                                         >  1:127271/1‑251 (MQ=255)                                                                                aGATGAGCTAGGAATAGGGGACACGCTAGGATACATGTTGCAGCAGGAAGGTTTTGACGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGGATACA‑GACGTCATGAATCGCGATGTTGGTTTGCTAGATATCAGCGGCTTTACATTATGCAGCCAGTTACTGGAGATCCATACGGCGTTACCTGAACTGATCCTGACGGCCCGAAGGGGAGAGGTCGATCGCCTGCCTGGGc                                                                                                                                                                >  2:58798/1‑251 (MQ=255)                                                                                  agtaGCAAGGGATAGCCGACACGCTGGTCTACATGTGGGTGTAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCGTGATAAAGCTCGCCAGCAGG‑CTCCCGGCGTCATGATTCTCGATGATGGTTTGCCTGATACCAGCGGCTTTGAATTATGCCGCTAGTTGCTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGTCTCGAAGTGAAGAGGTCCATCGCCTGCTTGGGcgg                                                                                                                                                              <  2:33071/248‑3 (MQ=255)                                                                                     ggCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTTCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGaa                                                                                                                                                           <  1:279421/250‑1 (MQ=255)                                                                                        aaGGGATAGCCGCCACGATGGTCTACATGTTGCAGCAGGAAGGATTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGTCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGAAGGCCCGAAGTGAAGAGGTAGATCGCCTGCTTGGGCTGGAAAtt                                                                                                                                                        >  2:289076/1‑251 (MQ=255)                                                                                            gATAGCCGACACGCTGGGCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGATTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTg                                                                                                                                                    <  1:240390/251‑1 (MQ=255)                                                                                             aTAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGc                                                                                                                                                   <  1:67820/251‑1 (MQ=255)                                                                                               aGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTg                                                                                                                                                 <  2:150012/251‑1 (MQ=255)                                                                                                                aCATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGACTGATATCAGCGGCTTTGAATTATGCCGCAAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGCGACGAGGCCGATCGCCTGCTTGGGCTGGAAATTTGTGCGGACGACTATGTGGCTaa                                                                                                                                 >  2:105500/1‑250 (MQ=255)                                                                                                                             ggAAGGCTTCGCAGTCGAGGTCTTTGAGCGCGGCTTGCAGGTCCTGGATAAAGCTCGCAAGCAGG‑CTCCCGACGTCCTGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGATTTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGATACTGACGGCACGAAGTGAAGCGGTCGATCGCCTGCTTGGGATGGCACTTGGTGCTGACGACTATGTGGCAAAACCGTTTTCAcccc                                                                                                                   >  2:351702/1‑251 (MQ=255)                                                                                                                                         cGTCGAGGTCTTTGCGCTAGGCTTGCCGGTGCTGGATAAAGCACGCCAGCAGG‑CTCCCGACATGATGATTCTCGATGTTGTTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTTCTGGTGCTCCATCCGGTGTTACCTGCACTTTTCCTGACGGCCCGAAGTGAAGAGGTCGATCTCCTGCTTGGGCTGGAAATTGTGGCTGATGTCTATGTGGCTAAAGCGTTTTCatctcgattagtgtgc                                                                                                        <  2:332323/250‑16 (MQ=255)                                                                                                                                          gTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgcgc                                                                                                      <  1:380193/251‑1 (MQ=255)                                                                                                                                               ggTCATTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGAGGCTTTGAATTATGCCGCCAGTTACTGGCGCCCCATACGGCGTTACCTGTACTTTTCCTTACGCTCCAATGTGTAGATGGCGATCGCCTGCTTGGGCTGGAAATAGGTGCTAACCAGTATGTATCTACACCGTTTTCAcccaaagaactgctccccggtg                                                                                                 >  2:118215/1‑232 (MQ=255)                                                                                                                                                      gAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCa                                                                                           <  1:169337/250‑1 (MQ=255)                                                                                                                                                             gCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTg                                                                                   >  1:279904/1‑251 (MQ=255)                                                                                                                                                                           gATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTgaagaa                                                                     <  1:143261/251‑1 (MQ=255)                                                                                                                                                                               aaGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTGCCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTtc                                                                 <  1:337170/251‑1 (MQ=255)                                                                                                                                                                                 gCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTtctc                                                               <  1:373323/251‑1 (MQ=255)                                                                                                                                                                                          gcaggctcccGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGt                                                       <  1:289076/244‑1 (MQ=255)                                                                                                                                                                                             ggctcccGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGtctc                                                    >  1:221593/5‑251 (MQ=255)                                                                                                                                                                                                 cccGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCg                                                 <  1:148652/250‑1 (MQ=255)                                                                                                                                                                                                       gTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGACAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCg                                          <  2:108331/251‑1 (MQ=255)                                                                                                                                                                                                         cagtaTTCTCTATGTTGGTTTGCCTGATATCAGCGGCTTTGATTTATGCCGCCAGTTACAGGCGCTCCATCCGGCGTTGCCCGTACTGTTCCTGACGGCCCGCAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGt                                         <  2:127271/246‑1 (MQ=255)                                                                                                                                                                                                            gATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTAtt                                      <  1:351702/250‑1 (MQ=255)                                                                                                                                                                                                                               cctgATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacgac                                                                                                                                            <  1:324795/126‑1 (MQ=255)                                                                                                                                                                                                                               cctgATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGACGATCGCCTGCTTGGGCTGGAAATTGGTGCTgccgac                                                                                                                                            >  2:324795/4‑129 (MQ=255)                                                                                                                                                                                                                                ctgATATCAGCGGCTTTGAGTTATCCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTTTACTGTTCCTGACGGCCCGAAGTGAAGAGCTCGATCGCCTGATTGGGCTGGAAATTGGTGCTGACGACTCTGTGGCTAAACCGTTTTCACCCCACGAAGTGGGCGCCAGGGTGCGCCCCTTACAGCGTCGGGTGGAGAAGTTCTCGTCGCCGTCGCCGGTCATCCGTATTAGGCATTTTGATTtgaatgt                  >  2:24905/3‑249 (MQ=255)                                                                                                                                                                                                                                    tatcaGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCcg             >  1:322311/5‑251 (MQ=255)                                                                                                                                                                                                                                                 gAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATc  >  1:171704/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATC  >  NC_000913/4635957‑4636444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑