Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,132	A→C	G276G (GGT→GGG)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,132	0	A	C	100.0%	27.1 / NA	12	G276G (GGT→GGG)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/4);  total (8/4)

AATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGG  >  NC_000913/6889‑7357                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  aaTAACCACGAGGGGAACAAAATCAGCGCCCCAGGAACCCATAAACACACGCATAGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAAGGTGTGCCATTACCCACAAGTAATCTCAGCATGGCGCTTGCCGGACAGATGACCAGGGTGTCGGAAAAAATGCCAATAATCTGGACAAACCCGTGGGCTTCCGGATGAGGGGGACAGGCCGCCGCTACCGCTGCCCGGTTTGGAGGCGACCCCAtac                                                                                                                                                                                                                            >  2:306144/1‑249 (MQ=255)                                                                                   aggggatggtggttggtcacttcaCCCGACAGTAATATCAGAATGGCGTTTGCCGTACAGATGACTAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCGGCCGAATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGcc                                                                                                                                            <  1:179513/226‑1 (MQ=255)                                                                                   aGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGAACAGGGGGTAGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGGCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTAGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGAAATCGCCCGGCTTAAAGAATATCCCGCCGCGCCGCCTGCCGc                                                                                                                                          >  1:258626/1‑251 (MQ=255)                                                                                      ggCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGACAACCTACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTc                                                                                                                                       >  1:122845/1‑251 (MQ=255)                                                                                                      cGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGCCCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATATCCTGGCTTAAGGTATATCACGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAACAGc                                                                                                                        >  2:304319/1‑250 (MQ=255)                                                                                                                      aatCAGCATGGCTCTTCCCTTACAGATGACCTGGTTTTCGATAAAAATGCCAATCATCGGGACAACCCCTTGCGCTGCCGGATGCGGGGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGAcc                                                                                                       <  1:70026/250‑1 (MQ=255)                                                                                                                               ggcactcgccataagtaTGACCAGGGTGTCAATAAAAAGGCCATTCATCTGGCCAAACCCTTGCGCTCCCGGATGCGGGGGCAAGGTCGCCGCGGCCCCTGCCGCCTTTGGCTTCGACCCAATTCCCTCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTTGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACg                                                                                               <  1:372436/234‑1 (MQ=255)                                                                                                                                                          gtCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCAGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATAACGTGGGGAAGTTGCCCGATATTCATTACGc                                                                   >  1:45143/1‑251 (MQ=255)                                                                                                                                                                 aaaaTGACAATCATCCGGACAATCCCTTGCGCTGCCGGATGCAGAAGACCGGACGCCGCTGCAGCTTCCGCGTTTGGCGCCGCCCCCATTCCCGCCTCCTTGGCAACCATACTGCGCTGAAAACCGTTAGTAATCACCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCCCAAAAATAGACCAAAAGACGTGAGGAAGTAGCCCGCTCATCCTTACACAAATTAc                                                            >  2:47139/1‑251 (MQ=255)                                                                                                                                                                  aaaTGACAATCCTTTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCCCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCAcc                                                           <  1:136800/251‑1 (MQ=255)                                                                                                                                                                                                                 cAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCTACGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTAGCCCGCTATTCATTACGCCAGTCACCAGGCGGGTCCGTACCCAGATTATCGACATCAACggcaacaaccccgc            >  2:181972/1‑239 (MQ=255)                                                                                                                                                                                                                           ctgccgctgccgCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGACAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGAACCCAGATTATCCCCATCAACGGAACAAACCCCTGCATGAACCgg  >  2:111063/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  AATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGG  >  NC_000913/6889‑7357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑