Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,186	T→C	I60I (ATT→ATC)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,186	0	T	C	92.5%	97.2 / 0.4	40	I60I (ATT→ATC)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (16/21);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01

CGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGA  >  NC_000913/4635945‑4636428                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   cGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCttt                                                                                                                                                                                                                                           <  1:274046/251‑1 (MQ=255)        aaaCGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGCGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGCCGTCATGATTCTCGATGTTGGTTTGCATGATATCAGCGGCTTTGAAttat                                                                                                                                                                                                                                    >  1:169072/1‑249 (MQ=255)             cgATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTTCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTgccgc                                                                                                                                                                                                                               >  1:183358/1‑251 (MQ=255)             cgATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATgccgc                                                                                                                                                                                                                               <  1:128164/251‑1 (MQ=255)                  aGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCCTGATTCTCGATGTTGGTTTGCCTGCTATCAGCGGCTTTGAATTATGCCGCCGGtt                                                                                                                                                                                                                          >  2:181600/1‑251 (MQ=255)                                 aTGCCGTGGCGGCATAAACAGAGGCGATTTAGGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTTCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATcc                                                                                                                                                                                                           <  2:183358/251‑1 (MQ=255)                                 aTGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATcc                                                                                                                                                                                                           >  1:108331/1‑251 (MQ=255)                                       tggcgcaaaTAACAGAGGCGATTTATGCAACGGGACAAGGCATGGTTAGAGGAAGATGAGCAAGGGATAGACGACACGCTGGTCTCCATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGAGAGGATTGCCGGTGCTGGATAAAGCTCGCAATCAGG‑CACCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGCATTATGCCCACTGTTCCTGGCGCTCCATCCGGCGtt                                                                                                                                                                                                     >  2:163376/8‑251 (MQ=255)                                                      agGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCt                                                                                                                                                                                      <  1:20254/251‑1 (MQ=255)                                                      agGCGATTTATGCAACGGGAAACGGTCTGGATAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCt                                                                                                                                                                                      >  1:99509/1‑251 (MQ=255)                                                                   aacggggaccgttCTGGTTAGTGGAAGATGAGGAAGGGATAGCCGACACGCTGGGCTACATTTTGCAGCAGGAAGGTGTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGt                                                                                                                                                                         <  2:181463/239‑1 (MQ=255)                                                                            tggTCTGGTTAGTGGAAGATGAGAAAGGGATAGCCGACACGCTGGTCTACATGGTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGCGCTTGATAAAGCTCGCCAGCAGG‑CTCCCGACGGCATGATTGTCGATGTTGGTATGCCTGACATCAGCGGCTTTGAATACTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGAACTGTTCCTGACGGCCCGactcgaaggagtc                                                                                                                                                                >  2:376864/2‑239 (MQ=255)                                                                               gcTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCGGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGAt                                                                                                                                                             <  1:135023/250‑1 (MQ=255)                                                                                   gTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGcc                                                                                                                                                         >  1:127271/1‑251 (MQ=255)                                                                                            aGATGAGCTAGGAATAGGGGACACGCTAGGATACATGTTGCAGCAGGAAGGTTTTGACGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGGATACA‑GACGTCATGAATCGCGATGTTGGTTTGCTAGATATCAGCGGCTTTACATTATGCAGCCAGTTACTGGAGATCCATACGGCGTTACCTGAACTGATCCTGACGGCCCGAAGGGGAGAGGTCGATCGCCTGCCTGGGc                                                                                                                                                >  2:58798/1‑251 (MQ=255)                                                                                              agtaGCAAGGGATAGCCGACACGCTGGTCTACATGTGGGTGTAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCGTGATAAAGCTCGCCAGCAGG‑CTCCCGGCGTCATGATTCTCGATGATGGTTTGCCTGATACCAGCGGCTTTGAATTATGCCGCTAGTTGCTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGTCTCGAAGTGAAGAGGTCCATCGCCTGCTTGGGcgg                                                                                                                                              <  2:33071/248‑3 (MQ=255)                                                                                                 ggCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTTCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGaa                                                                                                                                           <  1:279421/250‑1 (MQ=255)                                                                                                    aaGGGATAGCCGCCACGATGGTCTACATGTTGCAGCAGGAAGGATTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGTCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGAAGGCCCGAAGTGAAGAGGTAGATCGCCTGCTTGGGCTGGAAAtt                                                                                                                                        >  2:289076/1‑251 (MQ=255)                                                                                                        gATAGCCGACACGCTGGGCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGATTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTg                                                                                                                                    <  1:240390/251‑1 (MQ=255)                                                                                                         aTAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGc                                                                                                                                   <  1:67820/251‑1 (MQ=255)                                                                                                           aGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTg                                                                                                                                 <  2:150012/251‑1 (MQ=255)                                                                                                                            aCATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGACTGATATCAGCGGCTTTGAATTATGCCGCAAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGCGACGAGGCCGATCGCCTGCTTGGGCTGGAAATTTGTGCGGACGACTATGTGGCTaa                                                                                                                 >  2:105500/1‑250 (MQ=255)                                                                                                                                         ggAAGGCTTCGCAGTCGAGGTCTTTGAGCGCGGCTTGCAGGTCCTGGATAAAGCTCGCAAGCAGG‑CTCCCGACGTCCTGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGATTTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGATACTGACGGCACGAAGTGAAGCGGTCGATCGCCTGCTTGGGATGGCACTTGGTGCTGACGACTATGTGGCAAAACCGTTTTCAcccc                                                                                                   >  2:351702/1‑251 (MQ=255)                                                                                                                                                     cGTCGAGGTCTTTGCGCTAGGCTTGCCGGTGCTGGATAAAGCACGCCAGCAGG‑CTCCCGACATGATGATTCTCGATGTTGTTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTTCTGGTGCTCCATCCGGTGTTACCTGCACTTTTCCTGACGGCCCGAAGTGAAGAGGTCGATCTCCTGCTTGGGCTGGAAATTGTGGCTGATGTCTATGTGGCTAAAGCGTTTTCatctcgattagtgtgc                                                                                        <  2:332323/250‑16 (MQ=255)                                                                                                                                                      gTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgcgc                                                                                      <  1:380193/251‑1 (MQ=255)                                                                                                                                                           ggTCATTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGAGGCTTTGAATTATGCCGCCAGTTACTGGCGCCCCATACGGCGTTACCTGTACTTTTCCTTACGCTCCAATGTGTAGATGGCGATCGCCTGCTTGGGCTGGAAATAGGTGCTAACCAGTATGTATCTACACCGTTTTCAcccaaagaactgctccccggtg                                                                                 >  2:118215/1‑232 (MQ=255)                                                                                                                                                                  gAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCa                                                                           <  1:169337/250‑1 (MQ=255)                                                                                                                                                                         gCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTg                                                                   >  1:279904/1‑251 (MQ=255)                                                                                                                                                                                       gATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTgaagaa                                                     <  1:143261/251‑1 (MQ=255)                                                                                                                                                                                           aaGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTGCCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTtc                                                 <  1:337170/251‑1 (MQ=255)                                                                                                                                                                                             gCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTtctc                                               <  1:373323/251‑1 (MQ=255)                                                                                                                                                                                                      gcaggctcccGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGt                                       <  1:289076/244‑1 (MQ=255)                                                                                                                                                                                                         ggctcccGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGtctc                                    >  1:221593/5‑251 (MQ=255)                                                                                                                                                                                                             cccGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCg                                 <  1:148652/250‑1 (MQ=255)                                                                                                                                                                                                                   gTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGACAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCg                          <  2:108331/251‑1 (MQ=255)                                                                                                                                                                                                                     cagtaTTCTCTATGTTGGTTTGCCTGATATCAGCGGCTTTGATTTATGCCGCCAGTTACAGGCGCTCCATCCGGCGTTGCCCGTACTGTTCCTGACGGCCCGCAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGt                         <  2:127271/246‑1 (MQ=255)                                                                                                                                                                                                                        gATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTAtt                      <  1:351702/250‑1 (MQ=255)                                                                                                                                                                                                                                           cctgATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacgac                                                                                                                            <  1:324795/126‑1 (MQ=255)                                                                                                                                                                                                                                           cctgATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGACGATCGCCTGCTTGGGCTGGAAATTGGTGCTgccgac                                                                                                                            >  2:324795/4‑129 (MQ=255)                                                                                                                                                                                                                                            ctgATATCAGCGGCTTTGAGTTATCCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTTTACTGTTCCTGACGGCCCGAAGTGAAGAGCTCGATCGCCTGATTGGGCTGGAAATTGGTGCTGACGACTCTGTGGCTAAACCGTTTTCACCCCACGAAGTGGGCGCCAGGGTGCGCCCCTTACAGCGTCGGGTGGAGAAGTTCTCGTCGCCGTCGCCGGTCATCCGTATTAGGCATTTTGATTtgaatgt  >  2:24905/3‑249 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   CGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGA  >  NC_000913/4635945‑4636428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑