Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,706	C→A	T62T (ACC→ACA)	creA →	PF05981 family protein CreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,706	0	C	A	97.0%	88.3 / ‑4.3	33	T62T (ACC→ACA)	creA	PF05981 family protein CreA
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (20/12);  total (21/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

AATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCC  >  NC_000913/4635462‑4635948                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   aaTATGTTATTTACCGTGACGAACTAATTGCTCGTGAACTAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTAATAATGCTGGGGCCATTGGATCATGCAGAAGAGATTGGATAGGTCTACACCGTATTTAAAATGATCGGCCCGGATCACAAACTTGTTGTGGAAGCCTATGACGAACCCGATGTGAAAAATGTCACCTGTTAGGAGAGCCGGGCGAAAACAggtggg                                                                                                                                                                                                                                              >  2:308941/1‑250 (MQ=255)           ttACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAggg                                                                                                                                                                                                                                     <  1:64544/250‑1 (MQ=255)                       aCTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTgg                                                                                                                                                                                                                        <  1:341693/251‑1 (MQ=255)                                   tgtAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCt                                                                                                                                                                                                            >  1:41117/1‑251 (MQ=255)                                       aTAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCg                                                                                                                                                                                                         >  1:219543/1‑250 (MQ=255)                                        tAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCATGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGACAGGATCACAAAATTGATGTGGAAGCCTTTGACAATACCGATGTGGAAAATGTCACCTGTTATGTGAGCCGGGCGAAACCAGGTGGTGGTAAAGGGGGATGGGGTCTGGCGGAAGAGAccgccaat                                                                                                                                                                                                       >  2:152241/1‑245 (MQ=255)                                          gATAAAAATTGTAACAATAGGAAATACAAGCATTTGAGCCTGTCTTTAAGCCTGATAATGCTGGGGCCTGTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATTATCGGCACGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTTTTATGTGAGCCGGGCGAAATCAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGAGACCTCCGATGc                                                                                                                                                                                                     <  2:298122/251‑1 (MQ=255)                                              aaaaTGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGct                                                                                                                                                                                                 >  1:137477/1‑250 (MQ=255)                                                              gAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGAGTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTATGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCTCCTGTGATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAg                                                                                                                                                                                 <  1:198143/251‑1 (MQ=255)                                                                             gATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAAc                                                                                                                                                                  >  1:78063/1‑251 (MQ=255)                                                                             gATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAAc                                                                                                                                                                  >  1:299502/1‑251 (MQ=255)                                                                              agcctttcgttAAGACTGATAATGCGGGGTCCATTGGCTCATGCTGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGATGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGTGTCTGGCGGATGTTACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACt                                                                                                                                                                 <  2:63421/242‑1 (MQ=255)                                                                                      tttAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATc                                                                                                                                                         >  1:331754/1‑251 (MQ=255)                                                                                               gATAATTCTGGGGTCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGGCACCTGTTATTTGAGCCGGGCGTAAACAGGTGGTAGTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAATTCGGGCCGATTGAACTGTCGGATCGTATTaa                                                                                                                                                  <  1:46258/249‑1 (MQ=255)                                                                                               gATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTaaa                                                                                                                                                 >  1:213279/1‑250 (MQ=255)                                                                                                  aaTGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCTGATCACAAAATTGTTGGTGAAGCCTTTTACGATCCCGATGTGAAAAATTTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGTGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACg                                                                                                                                             <  1:29135/251‑1 (MQ=255)                                                                                                              aTTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAgg                                                                                                                                 >  1:181463/1‑251 (MQ=255)                                                                                                                        gCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGgtagt                                                                                                                       >  1:227202/1‑251 (MQ=255)                                                                                                                                     gTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCCCCTGTTATGTGAGCAGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGCGGTAGTATTCaaaaaaagc                                                                                                          >  1:42663/1‑248 (MQ=255)                                                                                                                                             gccacCGTATTTAAAATGATCGGACCGGAGCACAAAATTGTTGTGGAAGCCTTTGACAATCTCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGTTGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGCGGTAGAATTCAAAAAACTCACGTcccc                                                                                                  >  2:62396/3‑250 (MQ=255)                                                                                                                                                    tATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAAAGCACGTCCCTGGTCttt                                                                                           >  1:274928/1‑251 (MQ=255)                                                                                                                                                     aTTTAAAATGAACTGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGGCACCTGTTATGTGAGCCGGGCGAAAAAAGGTGGGATTAAAGTGGGATTGGGTCTGGCGGCAGATACCTCAGATGAGGATATTACTTGTCAACAAGTCGAGGCGAATGAACTGTCGGGTCGTATTAAAAAAGGCTAAGCTCAGGGCGAGGGAGTATTCACAAAACTCAAGTACCTGGGCtta                                                                                           >  2:285272/1‑249 (MQ=255)                                                                                                                                                                     ccGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGAGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGGCGt                                                                          >  1:102842/1‑251 (MQ=255)                                                                                                                                                                          tCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTAGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGAAGTATTCAAAAAACGAACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCt                                                                     >  1:51162/1‑251 (MQ=255)                                                                                                                                                                                aaTTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGAATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGATTTTatg                                                               >  1:232030/1‑251 (MQ=255)                                                                                                                                                                                 aTTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTatga                                                              <  1:308941/251‑1 (MQ=255)                                                                                                                                                                                                 ttGACGATCCCGATGTGAATAATGTCAACAGTTAAGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTTTGGCGGAATATACCTCCGATGCTGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGGCGTGCGCTTTTATGACGCCAaacgcaacgc                                               <  2:41117/250‑1 (MQ=255)                                                                                                                                                                                                  tgacgatCCCGATGTGAAAAATGTCCCCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGTGCCGATTGAACTGTCGGATCGTATTAAAAACGTCAAAGCTCAGGTCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGTTCGTGCGCTTTTATGACGCCAAACGCAAcgcg                                              <  1:155492/246‑1 (MQ=255)                                                                                                                                                                                                                          cACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGgtagt                                                                                                                       >  1:263372/1‑153 (MQ=255)                                                                                                                                                                                                                          cACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGgtagt                                                                                                                       <  2:263372/153‑1 (MQ=255)                                                                                                                                                                                                                                       aGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAgg        >  1:33071/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccGGGCGAAACCAGGTGGTATTAAAGGTGGATTGGGTCTGGCGGAAGATACCTCAGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGCGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTAATCTGGCTTAATCCGACAAAGTTGtgtaagggt      >  2:148652/1‑243 (MQ=255)                                                                                                                                                                                                                                             gCGAAAACAGGTGGTATTAAAGGGGGATTTGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGcc  <  1:204809/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   AATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCC  >  NC_000913/4635462‑4635948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑