Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 6,933 G→A L343L (CTA→TTA)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009136,9330GA89.5% 41.8 / ‑2.3 19L343L (CTA→TTA) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (13/4);  total (15/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTC  >  NC_000913/6706‑7139
                                                                                                                                                                                                                                    |                                                                                                                                                                                                               
gCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGGGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATa                                                                                                                                                                                           <  1:365025/250‑1 (MQ=255)
  aGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCATAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGc                                                                                                                                                                                        >  1:2346/1‑251 (MQ=255)
                             acaTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGtt                                                                                                                                                            >  1:351439/1‑251 (MQ=255)
                                                 aaGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTa                                                                                                                                         >  1:105792/1‑250 (MQ=255)
                                                                                   tCCGCAAACACAAGATCGCTTTAAGGGTGTTCAGGCGTAATATGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCAATTAAAACCCGCATCGCCTTCTGGATAAGCTGAATCCATTCC‑AGCGGCTTGTAGATTGTGCCGGTAAACGCCAGTAATATCAGCATGCCGCtgtacgtacagcagaccacg                                                                                                      >  2:45075/1‑232 (MQ=255)
                                                                                                    gCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaaacttcc                                                                                     >  1:61594/1‑246 (MQ=255)
                                                                                                                 tCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCCGGGTGTCGATAAAAATGCCAATCATCTGGAc                                                                         >  1:204960/1‑250 (MQ=255)
                                                                                                                         aaaaaGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATc                                                                                                                                    >  1:38202/1‑183 (MQ=255)
                                                                                                                          aaaaGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTgc                                                               >  1:15113/1‑251 (MQ=255)
                                                                                                                              gaagaGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCt                                                                                                                         >  1:367614/1‑189 (MQ=255)
                                                                                                                               aagaGATTGTGTTCGGCGTAAAGTTAGTTGGCAACGATGGAGCGAAAGGAAAAAAAAAAAACCAGGAGGATAAAAAA‑CCCAGCCCCCCAGAAACCCTTTAACCCCCGCTTCGCCTTCGGGAAAAGCTGAAT‑CCTTCCCAGGGGAATGTGGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGc                                                          <  1:49993/251‑1 (MQ=255)
                                                                                                                                     tggtgcccggcgaataTGTATTTGACAACGATGGAGCAGAAGTAAAACAGAATAACCGCGAGG‑TAACAAAACACACCCCCCCAGGACCCAATAAACCCCCGCATCGCCTGCTGTATAAGCTAAATCCCTCCC‑AGCGGCATGGAGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGAt                                                     <  1:79035/235‑1 (MQ=255)
                                                                                                                                        ttttCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCgg                                                 >  1:205465/1‑251 (MQ=255)
                                                                                                                                         tttCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGCTAAAAATGCCAATCATCTGGACAATCCCTTGCTCTGCCGGATGCgg                                                 >  1:78388/1‑250 (MQ=255)
                                                                                                                                         tttCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTT‑CCGTACAGATTACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCgg                                                 >  1:218889/1‑249 (MQ=255)
                                                                                                                                                                         cTGAAGGCAAACAGAATAACCACGAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCATCTGGATCAGCTGAATACCTTCC‑AGCGGCATGTAGGGTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTgg                >  1:174866/1‑251 (MQ=255)
                                                                                                                                                                                       aaTAACCACGAGGGGAACAAA‑ATCAGCGCCCCAGGAACCCATAAACACACGCATAGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAAGGTGTGCCATTACCCACAAGTAATCTCAGCATGGCGCTTGCCGGACAGATGACCAGGGTGTCGGAAAAAATGCCAATAATCTGGACAAACCCGTGGGCTTCCGGATGAGGGGGACAGGCCGCCGCTACCGCTGCCCGGTTTGGAGGCGACCCCAtac  >  2:306144/1‑249 (MQ=255)
                                                                                                                                                                                                 aGGGTAACAAA‑CTCAGCACCCCAGGACCCAATTAACCCCCGCACCGCCTTCGGGAAAAGCTGAATCCCTCCC‑AGCGGCATGTAGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAAt                                                                                        <  1:165615/155‑1 (MQ=255)
                                                                                                                                                                                                 aGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAAt                                                                                        >  2:165615/1‑155 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                               
GCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAA‑CTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTC  >  NC_000913/6706‑7139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑