Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,631,245 C→T S171S (AGC→AGT slt → soluble lytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,2450CT93.6% 119.6 / 0.2 48S171S (AGC→AGTsltsoluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (23/21);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

CGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGC  >  NC_000913/4631000‑4631490
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
cgcgCTAACCCCACGCGTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCt                                                                                                                                                                                                                                                    <  1:50389/250‑1 (MQ=255)
         cccACGCTTCCTCCCGCTCGCACGCTGCAATCTCTTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTc                                                                                                                                                                                                                                          <  1:62505/251‑1 (MQ=255)
         cccACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTc                                                                                                                                                                                                                                          <  1:121632/251‑1 (MQ=255)
                    tccCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACa                                                                                                                                                                                                                                 >  1:324893/1‑249 (MQ=255)
                        gCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATcc                                                                                                                                                                                                                           >  1:159138/1‑251 (MQ=255)
                           cGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCg                                                                                                                                                                                                                          <  1:287179/249‑1 (MQ=255)
                                                     tGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGGGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGGATTTAGAGCGTATCCGTCTgg                                                                                                                                                                                              >  1:130326/1‑251 (MQ=255)
                                                                ggcgTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGGCTGGCGATGAAAGCg                                                                                                                                                                                   >  1:326113/1‑251 (MQ=255)
                                                                ggcgTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCACGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGTGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCg                                                                                                                                                                                   >  1:298310/1‑251 (MQ=255)
                                                                      aaGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAac                                                                                                                                                                             >  1:178702/1‑251 (MQ=255)
                                                                          cTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGACGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACCAGTTATTTAGTGTCTGGCGTGCGTCAGGAAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAaccctg                                                                                                                                                                         >  1:25195/1‑247 (MQ=255)
                                                                                         aGCCTTTGGCCCGGAAAAGCCCGGAACTACAGAATCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCTTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTg                                                                                                                                                           <  2:291028/250‑1 (MQ=255)
                                                                                              ttAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATcc                                                                                                                                                                                                    >  1:301952/1‑204 (MQ=255)
                                                                                              ttAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATcc                                                                                                                                                                                                    <  2:301952/204‑1 (MQ=255)
                                                                                                   ccGGAAAATACCGGAACTACCGAAGCGCAAGGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTTAAGAAGCATGGCAAGGGGCGAAAGAGCTGTGGCTACCCGGCAAGAGCCAGCCTAACTCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCTGATAACACTGGCCTGGTAACAGTGCTggcagggca                                                                                                                                                <  2:82991/251‑1 (MQ=255)
                                                                                                                      ccGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGCCAGGGCAGATGCCTGCCGATTAcc                                                                                                                               >  1:164915/1‑249 (MQ=255)
                                                                                                                                aTGTAATTACTTCTATGCTAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTTGCGTGCGTCAGGTACACAAGATCCGCTGGCGTATTTATAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGTCCTGGTCACAGTGCTGGCAGGTCAGATGCCTGCCGATTACCAGACTATCGCCt                                                                                                                   <  1:11027/251‑1 (MQ=255)
                                                                                                                                aTGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGt                                                                                                                                                                                                  >  1:276518/1‑172 (MQ=255)
                                                                                                                                aTGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGt                                                                                                                                                                                                  <  2:276518/172‑1 (MQ=255)
                                                                                                                                  gTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCg                                                                                                                 >  1:26648/1‑251 (MQ=255)
                                                                                                                                     aTTACTACTATGCAAAATGGAACACCGGGCAGAGTGCAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGACAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGGAGAAAGCGGGTAACACTGGCCTGGTAAAAGTGCTGGCAGGGCAGGTGCCGGCCGCTTAcccgcaacgcgcctcggc                                                                                                               >  2:206198/1‑234 (MQ=255)
                                                                                                                                        actactATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGCGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATc                                                                                                           >  1:349986/1‑251 (MQ=255)
                                                                                                                                        actactATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATc                                                                                                           <  1:206198/251‑1 (MQ=255)
                                                                                                                                                      tGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCCGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGa                                                                                             >  1:376924/1‑250 (MQ=255)
                                                                                                                                                       ggAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGaac                                                                                           <  1:255712/251‑1 (MQ=255)
                                                                                                                                                              cgtgCAGAGTGACGAAGCCTGGCAAGGGTCGAAAGAGCTGGGGCCAACCGGCAAGAGCCAGCCTAACGCCTGTGACCAGTTATCTAGTGCCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCTTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTa                                                                                    <  1:142750/248‑1 (MQ=255)
                                                                                                                                                              cGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCCGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTa                                                                                    <  1:64663/251‑1 (MQ=255)
                                                                                                                                                                        gaagaaGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTTACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGAGTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACt                                                                          <  1:261298/251‑1 (MQ=255)
                                                                                                                                                                              gacTGGAAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATACGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCTCCTCGGCAATCCTTTCCCGGGCGCACAAACCTCATACGGTACTGACCtt                                                                    >  2:94354/3‑251 (MQ=255)
                                                                                                                                                                                 tGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTcgc                                                                 >  1:169138/1‑251 (MQ=255)
                                                                                                                                                                                    cAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTcgcgcg                                                              >  1:120966/1‑251 (MQ=255)
                                                                                                                                                                                      aGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGtc                                                            >  1:75683/1‑250 (MQ=255)
                                                                                                                                                                                           cGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACt                                                       <  1:122833/251‑1 (MQ=255)
                                                                                                                                                                                                 agCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGcgcg                                                 <  1:349924/251‑1 (MQ=255)
                                                                                                                                                                                                    tggggCTAACCGGCAAGGGCCAGCCTAACGCCTGTGACAAGTTATTTATTGTCGGGCGTGCGTCAGGTAAACAAGACCCGCTGGCGTATTTAGAACGTAGCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGGCCTGACCTTCGCGCGTACAACTGGCGCGAc                                               <  2:159138/247‑1 (MQ=255)
                                                                                                                                                                                                     gtgGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGTCCTTCGCGCGTACAACTGGCGCGACCg                                             <  1:129666/251‑1 (MQ=255)
                                                                                                                                                                                                          tAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGAtttt                                        >  1:194175/1‑251 (MQ=255)
                                                                                                                                                                                                           aaCCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAAAAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGAtttt                                        >  1:343820/1‑250 (MQ=255)
                                                                                                                                                                                                           aaCCGGCAAAAGCCAGCCCAACGCCTTGGACAAGTTATTTAGTGTCGGGCGGGCGTCAGGTAAACAAGATCCGCGGGCTGATTTATAGCTTATCCGTCTGGCGATGAAAGCGGGGAACACTGGCCTGGTAACAGTGCTTGCAGTGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGCCCTTCGCGCGTACAACGGGCGCGACCGAttt                                         <  2:349986/249‑1 (MQ=255)
                                                                                                                                                                                                                  aaGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTc                                 >  1:17810/1‑250 (MQ=255)
                                                                                                                                                                                                                       ccagccTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATgg                           <  1:207198/251‑1 (MQ=255)
                                                                                                                                                                                                                        cagccTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTAATGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCAGTAAAATggg                          >  1:183241/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gtgtccaGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCAGATGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCATGCCGATTACCAGACCATCGCCTCGGCAATGATTTACATGGCGACAAACCCTAATACGAGACTGAACTTCGAGCGGACAAAAGGGCCGACCGACTTTACCCATAACAAGGCGGCGGGGGCggtt             >  2:269706/7‑248 (MQ=255)
                                                                                                                                                                                                                                     gagACAAGTTAGTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGGATTTAGAGCGTATCCGTCTTGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGtt              <  1:267385/248‑1 (MQ=255)
                                                                                                                                                                                                                                            gTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAggg      >  1:221872/1‑249 (MQ=255)
                                                                                                                                                                                                                                                tttAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGCGCGTATCCTGCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGAAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCCATCATTTCACTGGCGACCAACCCTAATACGGGACTGACCTTCGCGCTTACAACTTACGCGACCGATTTTACCCGACAAATGGCGGGGGTgtggttttgcagcggtgg  >  2:88202/1‑234 (MQ=255)
                                                                                                                                                                                                                                                tttAGTGTCTGGCGTGCGTCAGGAAAACAAGATCCGCTGGCGGATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAAAACTGGCCTGGAAACAGTGCTGGCAGGGAAGATGCATGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACCACCCCAATCCGGTACTGACATTTGCGCATACAACTGGCGCCACCGATTTTAACCGTCAAATGGgggcggggggggtgggccatggggg  >  2:247290/1‑232 (MQ=255)
                                                                                                                                                                                                                                                 ttAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGgc  <  1:247290/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
CGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGC  >  NC_000913/4631000‑4631490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: