| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,630,960 | T→C | D76D (GAT→GAC) | slt → | soluble lytic murein transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,630,960 | 0 | T | C | 94.1% | 95.7 / ‑4.1 | 34 | D76D (GAT→GAC) | slt | soluble lytic murein transglycosylase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (16/16); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCT > NC_000913/4630715‑4631202 | aaattAGATGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgat < 1:257291/248‑1 (MQ=255) agagGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATc > 1:76005/1‑251 (MQ=255) cgcTTGTGGAAAACGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcggcg < 1:232798/251‑1 (MQ=255) tggggAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCTACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTCCCTGGAATACCGTCAGAGCACCGATGACTTGATGAATCAACCGGCGGt < 1:41860/246‑1 (MQ=255) ggAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGActg > 1:93071/1‑249 (MQ=255) cAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGAAGCGTAGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGATACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGCGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACATGATGAATCAACCGGCGGTGACTGTCTCTAACt > 1:182575/1‑251 (MQ=255) aaGTTTCCTGGCGGCTGTTGTCGGCCGGTGTCTGGCTGCTGACGGTCAGCAGCGTGGCGCGAGCCTACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACGTTATCCCTACCTGGAATACCGTCAGATCACCGTTGGCCTGATGAATCAACCGGCGGTGATTGTCACGAACTTTGtt < 2:192554/251‑1 (MQ=255) tGTTGGCTGCCGGTGACTGTCTGCTGACGGTCAGAAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGTCACTAACTTTGTTCGCGCTAAACCCACg > 1:109147/1‑251 (MQ=255) tGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTtcctc > 1:291028/1‑251 (MQ=255) tctgtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGc > 1:33422/1‑251 (MQ=255) gACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCa < 1:344879/249‑1 (MQ=255) tCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCACTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGGCTGTCACTAACTTTGTTCGCGCAAACCCCACGCTTCCTCCCACTCGCACGCTGCAATCTCGt > 1:114909/1‑251 (MQ=255) gAGCCGACGCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACtt < 1:235210/251‑2 (MQ=255) ggATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc > 1:148927/1‑221 (MQ=255) aTGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGAc < 1:146205/251‑1 (MQ=255) tGAGCAGCGTAGCCGTTCCGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCa > 2:171816/1‑147 (MQ=255) tGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCa < 1:171816/147‑1 (MQ=255) tGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc > 1:347843/1‑218 (MQ=255) ggAGCAGCGTAGCCGTGACGCGCAAATCAAGCAGGCCTGGTATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCTCTTTATCCCTACCTGGAATACCGTCAGATCTCCGATGACCTGATGAAGCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc < 2:347843/217‑1 (MQ=255) gTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGTTAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCAACTTTATCCCTACCTGGAATACCATCAGATCACAGATGACCTGATGAATCAACCGGCGGTGACTGTCCCTAACTTTTTTCGCGCTAAACCCACGCTTCCCCACACCCGCCCTCCGAAATCCCCTTTCGTCACTGAACTGGCGCGGCGTGAAGaacagcgtggg > 2:160982/1‑241 (MQ=255) aTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGccc < 1:47889/251‑1 (MQ=255) ggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGgc < 1:52515/198‑1 (MQ=255) aCAAATGGATGTGGTCGAACAAATGATGTCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCCTGTTAGCCTTTAGCCCGGGCAAAGCC‑GGAACTACCGa > 1:379634/1‑249 (MQ=255) aaTGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAGCCCGG‑GAAAGCCCGGAAATACCCAAgcgc > 1:238817/1‑251 (MQ=255) aacaaatatgtcttGGACTGAAGGATGTTTCGCTCTACCCTTACCTGGAATACCGCCAGTTCCCCGATGTTCTGATGAATCAACCGGTGGCGACGTTCACTAACTTTGTGCGCGCTAACCCCACGCTTCCTCCCGCTCGCAGGCTGCAATCTCGTTTCGTCAATGATCTGGCGCGGCGTGAAGACTGTCGTGTCTTGTTAGCCTTTATCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATtac < 2:131987/235‑1 (MQ=255) aaaaTGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATTCCGTCAGATCACCGAGGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTGTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACTCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTActacta < 1:160982/250‑1 (MQ=255) ggACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACAGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCTATGTAATTACTACTATGCAAAATGGa < 1:339407/250‑1 (MQ=255) cTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAACCCGGAACTACCGAAGCGCCATGTAATTAATACTATGCAAAATGGaaaac > 1:237233/1‑248 (MQ=255) aagATTTTCCACTTTATCCCTGCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCGCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCggg < 2:76005/249‑1 (MQ=255) ccacTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACGTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTga < 2:237233/248‑1 (MQ=255) caatgtagcccgaCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCTCGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTgaa < 2:152035/239‑1 (MQ=255) ccTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAg < 1:166629/251‑1 (MQ=255) aGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAAAGGAAAACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGGGAAagagatg > 1:147866/1‑248 (MQ=255) ccGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGGTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCTTGGGTTGTTTGACTTTAGACCGG‑GAAAGACCGGGAATCCCGAAACGCAATGGTATTACTACTATGCAAAATTGAACACCGGGCAGCGGGAAGAAGCCTGGCCAGGGGGGGAAGAGCTGTGGCt > 1:130496/1‑250 (MQ=255) | CACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGG‑AAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCT > NC_000913/4630715‑4631202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |