Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,615 C→T intergenic (‑93/‑118) ettA ← / → slt energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,6150CT93.8% 85.6 / ‑1.6 32intergenic (‑93/‑118)ettA/sltenergy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (17/13);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-02

ATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGC  >  NC_000913/4630368‑4630845
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      
aTAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCg                                                                                                                                                                                                                                     >  1:118417/1‑251 (MQ=255)
      cgcAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagta                                                                                                                                                                                                                                >  1:304178/1‑250 (MQ=255)
         agcagGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagtaaaag                                                                                                                                                                                                                            <  1:370394/251‑1 (MQ=255)
           cagGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTATTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAAAAACTTTGCCGCCACGTTGAATGGTATAAACGGATTGAGCAAAGTTGGACTTCGCCTATATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGAGTAGCCTTTTTCCCCGCCTATTCCCAGATGGACGATCACCTTCGCagtaaacgt                                                                                                                                                                                                                           >  1:236450/1‑247 (MQ=255)
              ggtagACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagtaaaagtaaa                                                                                                                                                                                                                        >  1:99837/5‑250 (MQ=255)
                  gACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATTACCTTCGCAGTAAAAGTAAAAAAgtg                                                                                                                                                                                                                  <  1:341423/251‑1 (MQ=255)
                                          aGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGc                                                                                                                                                                                            >  1:61042/1‑250 (MQ=255)
                                                       ttGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGc                                                                                                                                                                              >  1:141678/1‑251 (MQ=255)
                                                              aGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATc                                                                                                                                                                       >  1:192554/1‑251 (MQ=255)
                                                                    gaaACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAc                                                                                                                                                                 >  1:74786/1‑251 (MQ=255)
                                                                       aatCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACATCTTTGCCCACACGATGCATTGTATAAACGAAGTGAGCGACGTTGGACTTCGCCTCTATGTTTAACGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGTCTGGTTAGCATAAATCTATTAcgcg                                                                                                                                                              <  2:164892/249‑1 (MQ=255)
                                                                       aCTCAGAGAGATGTTTTACAAAATATGACGTTGCGGCGGAACAACTTTGCCGACACGATGAATGGTAGAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTGTATCGTGAGGAGGAGTTTTCAAAGGCGAAGTGTAGCCTGTTTCCCCGCCTAATCCCAGTTGGTCGATCTTCTTCGCAGTAAAAGTAAAAAAGGGACCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAcgcg                                                                                                                                                              <  1:50065/251‑1 (MQ=255)
                                                                              gagaGGTTTTTAAAAATTTGACGTTTCGACGGAACAACTTTTCCGTAGCGTTGCAGGGTAAAAACGATTTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGTCGAATTGAAGCCTTTTGCCCCGCCTAATCCCAGTTGGTCGATCGCCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACGGGTTAGCATAAATCTATTACGCGGCATGAc                                                                                                                                                       <  2:298332/251‑1 (MQ=255)
                                                                                 aTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTCCCGACACGATGCATGGTATAAACGATTTGAGCCACGTGGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCt                                                                                                                                                    <  2:74786/251‑1 (MQ=255)
                                                                                         cAAAATAGGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGa                                                                                                                                             <  1:150218/250‑1 (MQ=255)
                                                                                               atGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTAtt                                                                                                                                       <  1:336768/250‑1 (MQ=255)
                                                                                                                   agtgtccGACACGATGCATGTTATAAACGAATTGGGCCACGGTGGACATCGCCTCTATTATTATCGTGATGATATGTGTTCAAAGGTGTAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTTGCGTAAACTGCAATGACTTGTTAGCATAAATCTATTACGAGACATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCtt                                                                                                                   <  2:51801/245‑1 (MQ=255)
                                                                                                                                   cATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAAc                                                                                                   >  1:338386/1‑250 (MQ=255)
                                                                                                                                    aTGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCAAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATATATTACGCGGCAAGAAGCTGCATTGATGAATTTACCTTTAAACGATGCGCTTGTGGAAAAAGCAAAACa                                                                                                  >  2:262413/1‑250 (MQ=255)
                                                                                                                                                     aGCCACGTTGGACTTCGCCTCTATGATTATCGTGATGCTGAGTTTTCAAAAGCGAAGAGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTATAGGATGCGCTTGTGGAAAAAGCAAAACAAGTTACCTGGCGGCTGtt                                                                                >  1:106494/1‑251 (MQ=255)
                                                                                                                                                                acttccactttATGTTGATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCGTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGTCTGGTTAGCATAAATCTATTCCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTTCCGGGCGGCTTTTGGCTGCCGgtg                                                                     <  2:192329/241‑1 (MQ=255)
                                                                                                                                                                      ccTCTATGTTTATCGTGATGATGAGTTTTCAAATGCGAAGTGTAGCCTTTTTACCAGCCTAATCCCAGTTGGTCGCTCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGACATGACGCTGAATTGATGGATTTACACTTAGAGGAGGCGATTGTGGAAAAAGCCAACCAAGTTACCTGGCGGATGTTGGCTGCCGGcgtctgtc                                                               >  1:136396/1‑251 (MQ=255)
                                                                                                                                                                      ccTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGtgtctgtc                                                               >  1:339842/1‑251 (MQ=255)
                                                                                                                                                                               ttatcgggatgttGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACg                                                       <  2:339842/238‑1 (MQ=255)
                                                                                                                                                                                          aTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCAt                                                                                                                                                <  1:176999/150‑1 (MQ=255)
                                                                                                                                                                                          aTGAGTTTTCAAAGGCGAAGTGTACCCTTTTTCCCCGCCTAATCCCAGTTGGCCGATCACCTTCGCAGTAAAAGTAAAAAAGCGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCAt                                                                                                                                                >  2:176999/1‑150 (MQ=255)
                                                                                                                                                                                              gTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCACCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGgcgc                                       >  1:379291/1‑251 (MQ=255)
                                                                                                                                                                                                    aaaGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCAAATTCGCAGTAAAAGCAAAAAAGTGTACGTAACGTGGCGTAAACGGCAATGACTGGTTAGCCTAAATCTATTAAGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAACAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGGGCCg                                 >  2:47889/1‑251 (MQ=255)
                                                                                                                                                                                                                   ccTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCACTGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGGGTGGCGCGAGCCGACTCCCTGGAAGagc                  >  2:344879/1‑251 (MQ=255)
                                                                                                                                                                                                                       tttCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGagcagc               <  1:262413/250‑1 (MQ=255)
                                                                                                                                                                                                                            ccGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAGGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTAATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTc         >  1:131987/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aTCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAAGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTAcgc  >  1:342701/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      
ATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGC  >  NC_000913/4630368‑4630845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: