Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,553	A→G	intergenic (‑31/‑180)	ettA ← / → slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,553	0	A	G	100.0%	94.7 / NA	31	intergenic (‑31/‑180)	ettA/slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/13);  total (18/13)

ATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTC  >  NC_000913/4630317‑4630784                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        aTGTATGGCTGCGGACGCGCTTACCCTTCGATGTCTTTATCAATGACAGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCAGTTCAGCCCCAAGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATTTGACGTTTCGGCGGCACAACTTTGCCGACGCGATGCATGGAATAAACGAATTGAGCCCCGTTGGCCTTCGCCTCAATGTTTATCGTGAGGATGAGTTTTCaa                                                                                                                                                                                                                             >  2:218800/1‑249 (MQ=255)                     tccACCTTCGTTGTCTTAATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTc                                                                                                                                                                                                        <  1:218800/248‑1 (MQ=255)                                    tttATCAATGCCCGCCATCATGCGCAGCCGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAgt                                                                                                                                                                                        >  1:117077/1‑250 (MQ=255)                                      tATCAATACCCGCCATCATGCACAGCAGGGCCGACATCCCCGCGACGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGACGAAACTAAGAGAGATGTTATTCACAATATGACGTTTCGGCGGAACCACTTTGCCTACACGATGCATGGAATAACAGAAATGAACCACGATGGACTGCGCCTCTATGTTAATCGTGAAGATGAGTTTTCAAAAGCGAAGCGTATCCATATTCCACGACTAATccactatt                                                                                                                                                                                      >  2:257291/1‑245 (MQ=255)                                                    aTAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCg                                                                                                                                                                        >  1:118417/1‑251 (MQ=255)                                                      aaTGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTcccc                                                                                                                                                                                                     <  1:305812/220‑1 (MQ=255)                                                      aaTGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGGGTAGCCTTTTTcccc                                                                                                                                                                                                     >  2:305812/1‑220 (MQ=255)                                                          cgcAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagta                                                                                                                                                                   >  1:304178/1‑250 (MQ=255)                                                             agcagGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagtaaaag                                                                                                                                                               <  1:370394/251‑1 (MQ=255)                                                               cagGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTATTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAAAAACTTTGCCGCCACGTTGAATGGTATAAACGGATTGAGCAAAGTTGGACTTCGCCTATATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGAGTAGCCTTTTTCCCCGCCTATTCCCAGATGGACGATCACCTTCGCagtaaacgt                                                                                                                                                              >  1:236450/1‑247 (MQ=255)                                                                  ggtagACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagtaaaagtaaa                                                                                                                                                           >  1:99837/5‑250 (MQ=255)                                                                     agACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGttt                                                                                                                                                                                                                                 <  2:211960/176‑1 (MQ=255)                                                                     agACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGttt                                                                                                                                                                                                                                 >  1:211960/2‑177 (MQ=255)                                                                      gACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGA‑GAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATTACCTTCGCAGTAAAAGTAAAAAAgtg                                                                                                                                                     <  1:341423/251‑1 (MQ=255)                                                                                              aGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGc                                                                                                                               >  1:61042/1‑250 (MQ=255)                                                                                                           ttGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGc                                                                                                                 >  1:141678/1‑251 (MQ=255)                                                                                                                  aGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATc                                                                                                          >  1:192554/1‑251 (MQ=255)                                                                                                                        gaaACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAc                                                                                                    >  1:74786/1‑251 (MQ=255)                                                                                                                           aatCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACATCTTTGCCCACACGATGCATTGTATAAACGAAGTGAGCGACGTTGGACTTCGCCTCTATGTTTAACGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGTCTGGTTAGCATAAATCTATTAcgcg                                                                                                 <  2:164892/249‑1 (MQ=255)                                                                                                                           aCTCAGAGAGATGTTTTACAAAATATGACGTTGCGGCGGAACAACTTTGCCGACACGATGAATGGTAGAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTGTATCGTGAGGAGGAGTTTTCAAAGGCGAAGTGTAGCCTGTTTCCCCGCCTAATCCCAGTTGGTCGATCTTCTTCGCAGTAAAAGTAAAAAAGGGACCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAcgcg                                                                                                 <  1:50065/251‑1 (MQ=255)                                                                                                                                  gagaGGTTTTTAAAAATTTGACGTTTCGACGGAACAACTTTTCCGTAGCGTTGCAGGGTAAAAACGATTTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGTCGAATTGAAGCCTTTTGCCCCGCCTAATCCCAGTTGGTCGATCGCCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACGGGTTAGCATAAATCTATTACGCGGCATGAc                                                                                          <  2:298332/251‑1 (MQ=255)                                                                                                                                     aTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTCCCGACACGATGCATGGTATAAACGATTTGAGCCACGTGGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCt                                                                                       <  2:74786/251‑1 (MQ=255)                                                                                                                                             cAAAATAGGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGa                                                                                <  1:150218/250‑1 (MQ=255)                                                                                                                                                   atGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTAtt                                                                          <  1:336768/250‑1 (MQ=255)                                                                                                                                                                       agtgtccGACACGATGCATGTTATAAACGAATTGGGCCACGGTGGACATCGCCTCTATTATTATCGTGATGATATGTGTTCAAAGGTGTAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTTGCGTAAACTGCAATGACTTGTTAGCATAAATCTATTACGAGACATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCtt                                                      <  2:51801/245‑1 (MQ=255)                                                                                                                                                                                       cATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAAc                                      >  1:338386/1‑250 (MQ=255)                                                                                                                                                                                        aTGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCAAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATATATTACGCGGCAAGAAGCTGCATTGATGAATTTACCTTTAAACGATGCGCTTGTGGAAAAAGCAAAACa                                     >  2:262413/1‑250 (MQ=255)                                                                                                                                                                                                         aGCCACGTTGGACTTCGCCTCTATGATTATCGTGATGCTGAGTTTTCAAAAGCGAAGAGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTATAGGATGCGCTTGTGGAAAAAGCAAAACAAGTTACCTGGCGGCTGtt                   >  1:106494/1‑251 (MQ=255)                                                                                                                                                                                                                    acttccactttATGTTGATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCGTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGTCTGGTTAGCATAAATCTATTCCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTTCCGGGCGGCTTTTGGCTGCCGgtg        <  2:192329/241‑1 (MQ=255)                                                                                                                                                                                                                          ccTCTATGTTTATCGTGATGATGAGTTTTCAAATGCGAAGTGTAGCCTTTTTACCAGCCTAATCCCAGTTGGTCGCTCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGACATGACGCTGAATTGATGGATTTACACTTAGAGGAGGCGATTGTGGAAAAAGCCAACCAAGTTACCTGGCGGATGTTGGCTGCCGGcgtctgtc  >  1:136396/1‑251 (MQ=255)                                                                                                                                                                                                                          ccTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGtgtctgtc  >  1:339842/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        ATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTC  >  NC_000913/4630317‑4630784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑