Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,400	A→G	N41N (AAT→AAC)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,400	0	A	G	100.0%	86.8 / NA	31	N41N (AAT→AAC)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/9);  total (22/9)

TCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTG  >  NC_000913/4630167‑4630637                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               tCAAAATCGGCATCAGGATCGGCGTAAAGCGCATACACTTCCTCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGACTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGCCTTACCCACGCCGTTCAG‑ACCCAGGACAcc                                                                                                                                                                                                                              >  1:311688/1‑251 (MQ=255)                        gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAAACAATCTT‑GATGTC‑CGGATGCGGACATGCTTTAACTGCCATTGCTTTAACACTGCCCGCCATAATGCGCAGCAGGGAAGAATTACCAGCCCCGTGCAG‑ACCCAGGGCACCAATTTGTGCCCCAGGGAAGaaa                                                                                                                                                                                                        >  2:150218/1‑250 (MQ=255)                         accagcgataccctttcctccgggtgTTTCAGGGCGTTACCCCCTTCAAAATCTGCTTCTGCAATGGACCCCCGCAGGGGGTGTCCCGGGTTGACGTGCTTTCC‑CTGCGGAAGGTACCCAATTGT‑GA‑GTCCTGGCGGCGGACGCGCTCCACCTGGGACTCCTTTAACAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACt                                                                                                                                                                                                      <  1:199864/226‑1 (MQ=255)                            aGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTc                                                                                                                                                                                                     >  1:192329/1‑249 (MQ=255)                                catccacTACATCCAGGCGTTTCAGGGGGTTAACCCCTTCAAAACCCGTTTCTTCAAGGGCCTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑TGGGGGCAGTTAACCATTTTT‑GATGTC‑TGGCTGCGGCCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagagag                                                                                                                                                                                               <  1:73444/247‑1 (MQ=255)                                              aGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGGAGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCCG‑ACCCAAGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAat                                                                                                                                                                                 >  1:293190/1‑251 (MQ=255)                                                             ttAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑Accc                                                                                                                                                                                                                                      >  1:209979/1‑183 (MQ=255)                                                                          aaaCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCg                                                                                                                                                     >  1:16872/1‑251 (MQ=255)                                                                                cttcttCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑CCCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACg                                                                                                                                                >  1:200235/1‑250 (MQ=255)                                                                                     tcAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGGAACCAATCTT‑GCTGTC‑TGGCTGCGGGCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCCGGGTCGACTTACCCGCGCCGTT‑AGTACCAAGGGCACCAATTTTTGCCCCAGGGAAGAACATCAGAGAGATGTTTTTAAACATATGACGATTCGACGGAACACCTTTACCGacccgctgac                                                                                                                                           >  2:336768/1‑242 (MQ=255)                                                                                               cacgcacgGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCCGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGCCGTTTCGGCGGGACAACTTTGCCGACACGATTCATGGTATAAACg                                                                                                                                >  1:68794/1‑251 (MQ=255)                                                                                                  gcacgGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCCG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACAACATGCATGGGATAAACgga                                                                                                                              >  1:238533/1‑248 (MQ=255)                                                                                                   cgtggTGTGTTCTGGGGGCAGCTGCG‑TTCTCCGCGGCAGGTAACCAATTTTTGTTG‑C‑TGGCGGCGGACGCGCTTCCCCTTCGGTGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAAtt                                                                                                                            <  1:263191/248‑1 (MQ=255)                                                                                                                                  cTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGttt                                                                                               >  1:164892/1‑250 (MQ=255)                                                                                                                                      cggcaggtaaccattttttagttC‑TGGCGGGGGACGCGTTCCACCTCCGATGTCTTTATCAAGACCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCg                                                                                           <  1:250532/229‑1 (MQ=255)                                                                                                                                                 actCTT‑GATGTC‑TGGCTGCGGACACGCTTCACCTTCGATGTCTTTATCAATGCCCGCCCTAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGACCCAGGGAAGAAACTCAGAGAGCTGGTTTTCAAAATATGACGATTCGGCGGAACAACTTCGCCGCCACGATTCCTGGGATACACGTATTGAGAAACGTTGGACTTCGCCTCTATGTTTAtgaggatgatgagtt                                                                               >  2:149516/3‑237 (MQ=255)                                                                                                                                                         aTGTA‑TGGCTGCGGACGCGCTTACCCTTCGATGTCTTTATCAATGACAGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCAGTTCAG‑CCCCAAGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATTTGACGTTTCGGCGGCACAACTTTGCCGACGCGATGCATGGAATAAACGAATTGAGCCCCGTTGGCCTTCGCCTCAATGTTTATCGTGAGGATGAGTTTTCaa                                                                          >  2:218800/1‑249 (MQ=255)                                                                                                                                                                              tccACCTTCGTTGTCTTAATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTc                                                     <  1:218800/248‑1 (MQ=255)                                                                                                                                                                                             tttATCAATGCCCGCCATCATGCGCAGCCGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAgt                                     >  1:117077/1‑250 (MQ=255)                                                                                                                                                                                             tttATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCAAGAACACCAATTTTTGCCCAAGGGAAAAAACTCAGAGCGATGTTTTTCAAAATATGACGTTTAGGAGGAACAACTTTGCCGAAACGTTGCGTGGTTTAAACGAATTGGGCCACGTTGGATTTCGCCTCTATGTTTa                                                                                              >  2:247605/1‑194 (MQ=255)                                                                                                                                                                                               tATCAATACCCGCCATCATGCACAGCAGGGCCGACATCCCCGCGACGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGACGAAACTAAGAGAGATGTTATTCACAATATGACGTTTCGGCGGAACCACTTTGCCTACACGATGCATGGAATAACAGAAATGAACCACGATGGACTGCGCCTCTATGTTAATCGTGAAGATGAGTTTTCAAAAGCGAAGCGTATCCATATTCCACGACTAATccactatt                                   >  2:257291/1‑245 (MQ=255)                                                                                                                                                                                                             aTAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCg                     >  1:118417/1‑251 (MQ=255)                                                                                                                                                                                                               aaTGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTcccc                                                  <  1:305812/220‑1 (MQ=255)                                                                                                                                                                                                               aaTGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGGGTAGCCTTTTTcccc                                                  >  2:305812/1‑220 (MQ=255)                                                                                                                                                                                                                   cgcAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagta                >  1:304178/1‑250 (MQ=255)                                                                                                                                                                                                                      agcagGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagtaaaag            <  1:370394/251‑1 (MQ=255)                                                                                                                                                                                                                        cagGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTATTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAAAAACTTTGCCGCCACGTTGAATGGTATAAACGGATTGAGCAAAGTTGGACTTCGCCTATATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGAGTAGCCTTTTTCCCCGCCTATTCCCAGATGGACGATCACCTTCGCagtaaacgt           >  1:236450/1‑247 (MQ=255)                                                                                                                                                                                                                           ggtagACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagtaaaagtaaa        >  1:99837/5‑250 (MQ=255)                                                                                                                                                                                                                              agACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGttt                                                                              <  2:211960/176‑1 (MQ=255)                                                                                                                                                                                                                              agACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGttt                                                                              >  1:211960/2‑177 (MQ=255)                                                                                                                                                                                                                               gACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGA‑GAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATTACCTTCGCAGTAAAAGTAAAAAAgtg  <  1:341423/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               TCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTG  >  NC_000913/4630167‑4630637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑