Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,385 G→A S46S (TCC→TCT)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,3850GA92.6% 65.6 / NA 27S46S (TCC→TCT) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/7);  minor base C (2/0);  total (20/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGT  >  NC_000913/4630136‑4630628
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    
ttCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑TTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTGGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTgg                                                                                                                                                                                                                                                     >  1:23055/1‑251 (MQ=255)
                               tCAAAATCGGCATCAGGATCGGCGTAAAGCGCATACACTTCCTCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGACTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGCCTTACCCACGCCGTTCAG‑ACCCAGGACAcc                                                                                                                                                                                                                     >  1:311688/1‑251 (MQ=255)
                                                      gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAAACAATCTT‑GATGTC‑CGGATGCGGACATGCTTTAACTGCCATTGCTTTAACACTGCCCGCCATAATGCGCAGCAGGGAAGAATTACCAGCCCCGTGCAG‑ACCCAGGGCACCAATTTGTGCCCCAGGGAAGaaa                                                                                                                                                                                               >  2:150218/1‑250 (MQ=255)
                                                       accagcgataccctttcctccgggtgTTTCAGGGCGTTACCCCCTTCAAAATCTGCTTCTGCAATGGACCCCCGCAGGGGGTGTCCCGGGTTGACGTGCTTTCC‑CTGCGGAAGGTACCCAATTGT‑GA‑GTCCTGGCGGCGGACGCGCTCCACCTGGGACTCCTTTAACAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACt                                                                                                                                                                                             <  1:199864/226‑1 (MQ=255)
                                                          aGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTc                                                                                                                                                                                            >  1:192329/1‑249 (MQ=255)
                                                              catccacTACATCCAGGCGTTTCAGGGGGTTAACCCCTTCAAAACCCGTTTCTTCAAGGGCCTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑TGGGGGCAGTTAACCATTTTT‑GATGTC‑TGGCTGCGGCCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagagag                                                                                                                                                                                      <  1:73444/247‑1 (MQ=255)
                                                                            aGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGGAGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCCG‑ACCCAAGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAat                                                                                                                                                                        >  1:293190/1‑251 (MQ=255)
                                                                                           ttAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑Accc                                                                                                                                                                                                                             >  1:209979/1‑183 (MQ=255)
                                                                                                        aaaCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCg                                                                                                                                            >  1:16872/1‑251 (MQ=255)
                                                                                                              cttcttCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑CCCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACg                                                                                                                                       >  1:200235/1‑250 (MQ=255)
                                                                                                                   tcAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGGAACCAATCTT‑GCTGTC‑TGGCTGCGGGCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCCGGGTCGACTTACCCGCGCCGTT‑AGTACCAAGGGCACCAATTTTTGCCCCAGGGAAGAACATCAGAGAGATGTTTTTAAACATATGACGATTCGACGGAACACCTTTACCGacccgctgac                                                                                                                                  >  2:336768/1‑242 (MQ=255)
                                                                                                                             cacgcacgGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCCGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGCCGTTTCGGCGGGACAACTTTGCCGACACGATTCATGGTATAAACg                                                                                                                       >  1:68794/1‑251 (MQ=255)
                                                                                                                                gcacgGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCCG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACAACATGCATGGGATAAACgga                                                                                                                     >  1:238533/1‑248 (MQ=255)
                                                                                                                                 cgtggTGTGTTCTGGGGGCAGCTGCG‑TTCTCCGCGGCAGGTAACCAATTTTTGTTG‑C‑TGGCGGCGGACGCGCTTCCCCTTCGGTGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAAtt                                                                                                                   <  1:263191/248‑1 (MQ=255)
                                                                                                                                                                cTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGttt                                                                                      >  1:164892/1‑250 (MQ=255)
                                                                                                                                                                    cggcaggtaaccattttttagttC‑TGGCGGGGGACGCGTTCCACCTCCGATGTCTTTATCAAGACCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCg                                                                                  <  1:250532/229‑1 (MQ=255)
                                                                                                                                                                               actCTT‑GATGTC‑TGGCTGCGGACACGCTTCACCTTCGATGTCTTTATCAATGCCCGCCCTAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGACCCAGGGAAGAAACTCAGAGAGCTGGTTTTCAAAATATGACGATTCGGCGGAACAACTTCGCCGCCACGATTCCTGGGATACACGTATTGAGAAACGTTGGACTTCGCCTCTATGTTTAtgaggatgatgagtt                                                                      >  2:149516/3‑237 (MQ=255)
                                                                                                                                                                                       aTGTA‑TGGCTGCGGACGCGCTTACCCTTCGATGTCTTTATCAATGACAGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCAGTTCAG‑CCCCAAGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATTTGACGTTTCGGCGGCACAACTTTGCCGACGCGATGCATGGAATAAACGAATTGAGCCCCGTTGGCCTTCGCCTCAATGTTTATCGTGAGGATGAGTTTTCaa                                                                 >  2:218800/1‑249 (MQ=255)
                                                                                                                                                                                                            tccACCTTCGTTGTCTTAATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTc                                            <  1:218800/248‑1 (MQ=255)
                                                                                                                                                                                                                           tttATCAATGCCCGCCATCATGCGCAGCCGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAgt                            >  1:117077/1‑250 (MQ=255)
                                                                                                                                                                                                                           tttATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCAAGAACACCAATTTTTGCCCAAGGGAAAAAACTCAGAGCGATGTTTTTCAAAATATGACGTTTAGGAGGAACAACTTTGCCGAAACGTTGCGTGGTTTAAACGAATTGGGCCACGTTGGATTTCGCCTCTATGTTTa                                                                                     >  2:247605/1‑194 (MQ=255)
                                                                                                                                                                                                                             tATCAATACCCGCCATCATGCACAGCAGGGCCGACATCCCCGCGACGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGACGAAACTAAGAGAGATGTTATTCACAATATGACGTTTCGGCGGAACCACTTTGCCTACACGATGCATGGAATAACAGAAATGAACCACGATGGACTGCGCCTCTATGTTAATCGTGAAGATGAGTTTTCAAAAGCGAAGCGTATCCATATTCCACGACTAATccactatt                          >  2:257291/1‑245 (MQ=255)
                                                                                                                                                                                                                                           aTAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCg            >  1:118417/1‑251 (MQ=255)
                                                                                                                                                                                                                                             aaTGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTcccc                                         <  1:305812/220‑1 (MQ=255)
                                                                                                                                                                                                                                             aaTGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGGGTAGCCTTTTTcccc                                         >  2:305812/1‑220 (MQ=255)
                                                                                                                                                                                                                                                 cgcAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagta       >  1:304178/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    agcagGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagtaaaag   <  1:370394/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      cagGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTATTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAAAAACTTTGCCGCCACGTTGAATGGTATAAACGGATTGAGCAAAGTTGGACTTCGCCTATATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGAGTAGCCTTTTTCCCCGCCTATTCCCAGATGGACGATCACCTTCGCagtaaacgt  >  1:236450/1‑247 (MQ=255)
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    
TTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGT  >  NC_000913/4630136‑4630628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑