Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,304 A→G Y73Y (TAT→TAC)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,3040AG91.3% 46.0 / ‑1.4 24Y73Y (TAT→TAC) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (16/5);  total (17/7)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

CAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTT  >  NC_000913/4630056‑4630544
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   
cAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑TTGCGGCAGATa                                                                                                                                                                                                                                                   <  1:58972/251‑1 (MQ=255)
           ccGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑Ga                                                                                                                                                                                                                                       <  1:171118/250‑1 (MQ=255)
           aCGTAGCGCATCCGCCGCACGCTCCAGCTGAACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGACGTACAGCGCATACACTTCATCCACGCGTTTCAGGGCGTTAACCACTTCAGATACCGTTTCTTCCATCGACTCAAGCACGGTGTGTTCCGGGTTCAGCTGCGGATC‑CTCGGGCAGGTAACCAATCtta                                                                                                                                                                                                                                         >  2:250532/1‑249 (MQ=255)
                                      cTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCtt                                                                                                                                                                                                           >  1:51801/1‑251 (MQ=255)
                                              tCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCt                                                                                                                                                                                                   >  1:197682/1‑251 (MQ=255)
                                                 ggttATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTa                                                                                                                                                                                                <  1:196099/249‑1 (MQ=255)
                                                                gAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGTCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GAATTC‑TGGCTGCGTGCTCGCCTCACCTTCGATGTCTTTAtagaacggaaagggg                                                                                                                                                                                 >  2:298529/1‑237 (MQ=255)
                                                                gAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTAt                                                                                                                                                                                               <  1:298529/237‑1 (MQ=255)
                                                                                ttCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑TTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTGGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTgg                                                                                                                                                                 >  1:23055/1‑251 (MQ=255)
                                                                                           cTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGcagcag                                                                                                                                                                      >  1:267850/1‑235 (MQ=255)
                                                                                                               tCAAAATCGGCATCAGGATCGGCGTAAAGCGCATACACTTCCTCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGACTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGCCTTACCCACGCCGTTCAG‑ACCCAGGACAcc                                                                                                                                 >  1:311688/1‑251 (MQ=255)
                                                                                                                                      gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAAACAATCTT‑GATGTC‑CGGATGCGGACATGCTTTAACTGCCATTGCTTTAACACTGCCCGCCATAATGCGCAGCAGGGAAGAATTACCAGCCCCGTGCAG‑ACCCAGGGCACCAATTTGTGCCCCAGGGAAGaaa                                                                                                           >  2:150218/1‑250 (MQ=255)
                                                                                                                                       accagcgataccctttcctccgggtgTTTCAGGGCGTTACCCCCTTCAAAATCTGCTTCTGCAATGGACCCCCGCAGGGGGTGTCCCGGGTTGACGTGCTTTCC‑CTGCGGAAGGTACCCAATTGT‑GA‑GTCCTGGCGGCGGACGCGCTCCACCTGGGACTCCTTTAACAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACt                                                                                                         <  1:199864/226‑1 (MQ=255)
                                                                                                                                          aGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTc                                                                                                        >  1:192329/1‑249 (MQ=255)
                                                                                                                                              catccacTACATCCAGGCGTTTCAGGGGGTTAACCCCTTCAAAACCCGTTTCTTCAAGGGCCTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑TGGGGGCAGTTAACCATTTTT‑GATGTC‑TGGCTGCGGCCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagagag                                                                                                  <  1:73444/247‑1 (MQ=255)
                                                                                                                                                            aGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGGAGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCCG‑ACCCAAGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAat                                                                                    >  1:293190/1‑251 (MQ=255)
                                                                                                                                                                           ttAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑Accc                                                                                                                                         >  1:209979/1‑183 (MQ=255)
                                                                                                                                                                                        aaaCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCg                                                        >  1:16872/1‑251 (MQ=255)
                                                                                                                                                                                              cttcttCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑CCCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACg                                                   >  1:200235/1‑250 (MQ=255)
                                                                                                                                                                                                   tcAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGGAACCAATCTT‑GCTGTC‑TGGCTGCGGGCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCCGGGTCGACTTACCCGCGCCGTT‑AGTACCAAGGGCACCAATTTTTGCCCCAGGGAAGAACATCAGAGAGATGTTTTTAAACATATGACGATTCGACGGAACACCTTTACCGacccgctgac                                              >  2:336768/1‑242 (MQ=255)
                                                                                                                                                                                                             cacgcacgGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCCGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGCCGTTTCGGCGGGACAACTTTGCCGACACGATTCATGGTATAAACg                                   >  1:68794/1‑251 (MQ=255)
                                                                                                                                                                                                                gcacgGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCCG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACAACATGCATGGGATAAACgga                                 >  1:238533/1‑248 (MQ=255)
                                                                                                                                                                                                                 cgtggTGTGTTCTGGGGGCAGCTGCG‑TTCTCCGCGGCAGGTAACCAATTTTTGTTG‑C‑TGGCGGCGGACGCGCTTCCCCTTCGGTGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAAtt                               <  1:263191/248‑1 (MQ=255)
                                                                                                                                                                                                                                                cTGCGGCAGGTAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGttt  >  1:164892/1‑250 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   
CAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTC‑CTGCGGCAGATAACCAATCTT‑GATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAG‑ACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTT  >  NC_000913/4630056‑4630544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑