Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,238 T→A S95S (TCA→TCT)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,2380TA81.2% 63.0 / 9.4 32S95S (TCA→TCT) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base A (16/10);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

CAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAAT  >  NC_000913/4629995‑4630462
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 
ccggcggcAAAACGCTACGAGACGACGCTCACCACCGGAGAGGCTAGCGATTTTCGCGTCCCAGTCCGGCAGACGGAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCCGGTTATGACCGTCGAGAGACTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGCAGGACAGCGCATACACTTCATCCAGGCGTTTCAGGACGTTAACCACGTCAGAAAc                                                                                                                                                                                                                              >  2:170772/3‑249 (MQ=255)
     ggcACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGcttct                                                                                                                                                                                                                       <  1:21168/251‑1 (MQ=255)
               tACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGTCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACt                                                                                                                                                                                                             >  1:267630/1‑251 (MQ=255)
                   caacgcctctccccaacGGAGAGTTTAGCTATTTCTGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTGCAGGGTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTcacg                                                                                                                                                                                                         <  2:369613/235‑1 (MQ=255)
                    gacgacGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTATGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTcacgc                                                                                                                                                                                                        >  1:90104/1‑251 (MQ=255)
                    gacgacGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCATTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTcacgc                                                                                                                                                                                                        >  1:25917/1‑251 (MQ=255)
                                         ggTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGc                                                                                                                                                                                   >  1:185306/1‑251 (MQ=255)
                                            tAGCGCTTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCAAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCCGCGGCCAGCTTGTCACAATCGGCATCCGGATCGGCGTCCAGCGCATACACTTCATCCCGGAGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTAAGCTgg                                                                                                                                                                                >  2:122815/1‑250 (MQ=255)
                                                    ttCGCGTCCCAGTCCGGCAGACGGAGCGCATCCGCCGCACGCTCCAGCTGTATGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTctt                                                                                                                                                                         <  2:90104/250‑3 (MQ=255)
                                                    gtctcttcccatcccGGCAGACGATGCGCATCCGCCGCCCGCTCCAGTTGTACGTTCAGCTTATGACCGTCGTGAGCCTGAATGCTCTCTACCATACGGCCTTTTTCAGCGGCCAGTTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACGCTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTTAACCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCTGGTTCAGCCGCGGTTctt                                                                                                                                                                         <  2:25917/237‑3 (MQ=255)
                                                         gTCCCAGTCCGGCAGACGGAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCa                                                                                                                                                                   <  1:203501/251‑1 (MQ=255)
                                                            gctcttccgatctcGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAggt                                                                                                                                                                >  1:214976/14‑249 (MQ=255)
                                                             cAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAggta                                                                                                                                                               <  1:122815/251‑4 (MQ=255)
                                                             cAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATa                                                                                                                                                               <  1:58972/251‑1 (MQ=255)
                                                                        ccGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGa                                                                                                                                                    <  1:171118/250‑1 (MQ=255)
                                                                        aCGTAGCGCATCCGCCGCACGCTCCAGCTGAACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGACGTACAGCGCATACACTTCATCCACGCGTTTCAGGGCGTTAACCACTTCAGATACCGTTTCTTCCATCGACTCAAGCACGGTGTGTTCCGGGTTCAGCTGCGGATCCTCGGGCAGGTAACCAATCtta                                                                                                                                                     >  2:250532/1‑249 (MQ=255)
                                                                                       ctcACGCTCCAGTTGTACGTCCAGGTTATGACCTGAGTGTGTCTGAATGATCTCTGCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCTGCATCCGGATCGGCGTACATCGCAACCACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGTAACCGCTTCTTCAATGGa                                                                                                                                                                                                               <  2:335333/175‑1 (MQ=255)
                                                                                       cgcACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGa                                                                                                                                                                                                               >  1:335333/1‑177 (MQ=255)
                                                                                                   cTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCtt                                                                                                                        >  1:51801/1‑251 (MQ=255)
                                                                                                           tCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCt                                                                                                                >  1:197682/1‑251 (MQ=255)
                                                                                                              ggttATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTa                                                                                                             <  1:196099/249‑1 (MQ=255)
                                                                                                                             gAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGTCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGAATTC‑TGGCTGCGTGCTCGCCTCACCTTCGATGTCTTTAtagaacggaaagggg                                                                                              >  2:298529/1‑237 (MQ=255)
                                                                                                                             gAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTAt                                                                                                            <  1:298529/237‑1 (MQ=255)
                                                                                                                                             ttCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTGGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTgg                                                                              >  1:23055/1‑251 (MQ=255)
                                                                                                                                                        cTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGcagcag                                                                                   >  1:267850/1‑235 (MQ=255)
                                                                                                                                                                            tCAAAATCGGCATCAGGATCGGCGTAAAGCGCATACACTTCCTCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGACTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGCCTTACCCACGCCGTTCAGACCCAGGACAcc                                               >  1:311688/1‑251 (MQ=255)
                                                                                                                                                                                                   gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAAACAATCTTGATGTC‑CGGATGCGGACATGCTTTAACTGCCATTGCTTTAACACTGCCCGCCATAATGCGCAGCAGGGAAGAATTACCAGCCCCGTGCAGACCCAGGGCACCAATTTGTGCCCCAGGGAAGaaa                         >  2:150218/1‑250 (MQ=255)
                                                                                                                                                                                                    accagcgataccctttcctccgggtgTTTCAGGGCGTTACCCCCTTCAAAATCTGCTTCTGCAATGGACCCCCGCAGGGGGTGTCCCGGGTTGACGTGCTTTCCCTGCGGAAGGTACCCAATTGTGA‑GTCCTGGCGGCGGACGCGCTCCACCTGGGACTCCTTTAACAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACt                       <  1:199864/226‑1 (MQ=255)
                                                                                                                                                                                                       aGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTc                      >  1:192329/1‑249 (MQ=255)
                                                                                                                                                                                                           catccacTACATCCAGGCGTTTCAGGGGGTTAACCCCTTCAAAACCCGTTTCTTCAAGGGCCTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTGGGGGCAGTTAACCATTTTTGATGTC‑TGGCTGCGGCCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagagag                <  1:73444/247‑1 (MQ=255)
                                                                                                                                                                                                                         aGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGGAGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCCGACCCAAGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAat  >  1:293190/1‑251 (MQ=255)
                                                                                                                                                                                                                                        ttAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGAccc                                                       >  1:209979/1‑183 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 
CAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTC‑TGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAAT  >  NC_000913/4629995‑4630462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑