Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,887	A→C	V212V (GTT→GTG)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,887	0	A	C	100.0%	96.6 / NA	32	V212V (GTT→GTG)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/14);  total (18/14)

GCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGAC‑GTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCG  >  NC_000913/4629648‑4630115                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      gcCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCATACTTACCCACGCCAGCTCTTTCTCGATCGACATACGTCTCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGACTTCCCACGGAATACCTTCACCGCGGCCAAGTTCGAGGATCCAGCCTGCAACGTTATCGATGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCtt                                                                                                                                                                                                                             <  2:60055/250‑1 (MQ=255)  ccAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCg                                                                                                                                                                                                                           <  1:155267/251‑1 (MQ=255)    aGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCCCGGCATACCTTCACCGCGGTCCAGTTCGCGGATCCAGCCTGCAACGTTATCGCGGAAGTAACGGTCGTGGGTACTCGCCACCCCGGTGCCCTCGa                                                                                                                                                                                                                          >  2:150098/1‑250 (MQ=255)     gACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGa                                                                                                                                                                                                                          >  1:72629/1‑249 (MQ=255)                             ggCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATATTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGGGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTccagcc                                                                                                                                                                                                  >  1:226660/1‑249 (MQ=255)                                      ttccctGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGAGCCCTCGAAGTCGTGCAAGAAACGTTCCAGCCAGGCCACGGAt                                                                                                                                                                                       >  2:349988/6‑251 (MQ=255)                                       tccctGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGCTCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGAGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACggctt                                                                                                                                                                                      >  2:338524/5‑248 (MQ=255)                                        ccctGACGTACACACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTc                                                                                                                                                                                     >  1:134982/4‑251 (MQ=255)                                               gTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCCGGCCACGGATTCGGCATcc                                                                                                                                                                              >  1:248474/1‑251 (MQ=255)                                                tACCCACTCCAGCTCTTTCTCGATCGAATTACGACGCGCTGCTTCTTGTGCAGATTCCTGCGACAGGCGCTAACCTTTCTGATCCAGCCTGGAGGAGTAGTTGCCTTCCCACGGAATCACTTCACCGCGGTCAAGCTCGAGGATCCAGCCTGCAACGTTATCGAGGACGTAAGAGTCGTGAGTAATCGCAACCACGGTACCCTCGCAGACGGGAAGGAAGCGTTCAAGCCGGGCCACGCATTCGGcccca                                                                                                                                                                              >  2:249593/1‑246 (MQ=255)                                                         cAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGtggttgg                                                                                                                                                                    >  1:45584/1‑251 (MQ=255)                                                           gCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTc                                                                                                                                                                  <  1:296367/251‑1 (MQ=255)                                                                     tacccaCTTACCACCCGCCGCTTCTTGGTAATCTTCCTGCGCCAGGCGCTGAGTTTTCAGCCCCAGCCAGGATTAGTAGTTTCCCTCCCACGGAATCCCTTCACCGCGGTCAAGTTCTAGGTGCCAGCCTGCAACGTTATCGAGTAAGTAACGGTCGTTGGTAATCGCCACCACGGTGCCCGCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTgcgtcg                                                                                                                                                         <  2:326104/245‑1 (MQ=255)                                                                                        gcTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGttt                                                                                                                                     <  1:202590/251‑1 (MQ=255)                                                                                         cttcttGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGCAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGAAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCAACCGGGAGGTTGGTCGGTTCGTCGAgcagcagcagatctgggttt                                                                                                                                    >  2:83874/1‑240 (MQ=255)                                                                                                 gAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCcagc                                                                                                                              >  1:111651/1‑249 (MQ=255)                                                                                                        ccTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGATGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTCTCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGCTTCGGCATCCAGGTGGTTGGCCGGTTCGTCGCGCAGCAGAATGTCTGGTTTTTcacggagcgggagg                                                                                                                     >  2:237776/1‑238 (MQ=255)                                                                                                                            ttCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGcga                                                                                                      <  1:338524/246‑1 (MQ=255)                                                                                                                             tCTGCTCCAGCCAGGAGGAGTTGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGacgacg                                                                                                 <  1:284055/250‑1 (MQ=255)                                                                                                                               tGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCCCGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCCGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGacgacgctc                                                                                              >  1:20339/1‑248 (MQ=255)                                                                                                                                gCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCTCAACGCTACGCGACGACGCTca                                                                                             <  2:299106/251‑1 (MQ=255)                                                                                                                                   ccagccagGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTcacca                                                                                          <  1:240826/251‑1 (MQ=255)                                                                                                                                     agccagGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTTGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCaccacc                                                                                        <  1:216035/251‑1 (MQ=255)                                                                                                                                                      gccTTCCCACGGAATTCCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGAttt                                                                       <  1:329029/250‑1 (MQ=255)                                                                                                                                                                       cTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCCCACCGCTACGAGAAGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGACCCAGTCCGGc                                                      >  1:71182/1‑251 (MQ=255)                                                                                                                                                                       attCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGGTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCACTCGTAGACGTGCAGGAAGCGTTCCAGCCAGGCCACGGTTTCGGCATCCAGGTGGTTGGTCGTGTCGTCGAGCAGAAGCATGTCTGGTTTTTCCAGACGCAGGCGGCACACCGCTACGCGACGACGCTCACCACCGAAGCGGTTAGAGATTTTCGCGTCCCAGTCCGGc                                                      >  1:71188/2‑251 (MQ=255)                                                                                                                                                                                    aaGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGACGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACCCTACGCGACGACGCTCACCCCCGGAGAGGTTAGCGTTTTTCGCGTCCCCGTCCGGC‑GACCGTAGCGCAt                                        >  1:339263/1‑251 (MQ=255)                                                                                                                                                                                                  aGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGAC‑GTAGCGCATCCGCCGCACGCTcc                          >  1:298332/1‑251 (MQ=255)                                                                                                                                                                                                   gCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGAC‑GTAGCGCATCCGCCGCACGCTCCa                         <  1:82496/251‑1 (MQ=255)                                                                                                                                                                                                                 cGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGAC‑GTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAg            <  1:150098/250‑1 (MQ=255)                                                                                                                                                                                                                        gTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGAC‑GTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGAc    <  1:188476/251‑1 (MQ=255)                                                                                                                                                                                                                          aaCGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGAC‑GTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACcg  >  1:384866/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      GCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGAC‑GTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCG  >  NC_000913/4629648‑4630115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑