Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 6,850 A→G Y370Y (TAT→TAC)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009136,8500AG88.0% 51.1 / 1.1 25Y370Y (TAT→TAC) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (13/9);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

AAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAAT‑CACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGG  >  NC_000913/6601‑7092
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   
aatcgcagggagctcGCCGAGTTTACGCTGGAGTAGA‑AATGCGATGTCAATGGTATTAACCATATGTGAGAAAAGTAAAATTGCGTTCAAAATGGTAATAGCCATGCAGGCCATGATGAGATCTGTCAGTTTCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATTACCTTGGCGAAGGTGAAAATCCGCAAACACTTGACCGCTTTAGGGTTGTTCAGTCGTAAAAAGAATAGATTCCTTTCGGCaa                                                                                                                                                                                                                                                    <  2:94067/237‑2 (MQ=255)
          cgaaCGCCGAGTTTACGCTGGCGTAGATAAT‑CACTGGAAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGtt                                                                                                                                                                                                                                          <  1:280985/248‑1 (MQ=255)
                        aCGCTGGCGTAGATAAA‑CACTGGCAAGGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGc                                                                                                                                                                                                                            <  1:101925/251‑1 (MQ=255)
                              gCGTAGATAAT‑CACTGGCAATGGTATGAACCACAGGCGAGCGCAGTAAAATGGCGGTCAAATTGGGAATAGCCATGCAAGCCCTTATGATAACTGCCAGTTGCCCAAGCAGGGGAAGACTTAGACAGGCGCCGCCGGTGACCGTTTCGAAGGTGCAGAGCCGCAAAAACCAGAACGCCTTAGGGTTGTTTAGGGGTACAATGTAGAAACTGTTTTCAGCGTAAATTTCGATGGCAACGATgagggtgtg                                                                                                                                                                                                                        >  2:31168/1‑241 (MQ=255)
                                gTAGATAAT‑CACTGGCATGGGTATGAACCACAGGCTAGAGCATTAAAAGGGAGGTCAAATGGGTAATATCGATGCAGGGCATTATGATATCTGCCAGTGGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGAGTGTGTTCGGCATAAATTTAGTTGGCAACGATGGAGCTGAAGGCa                                                                                                                                                                                                                    <  2:141842/251‑1 (MQ=255)
                                    aTAAT‑CACTGGCAATTGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGTTCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTGCTGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACa                                                                                                                                                                                                                <  1:141323/251‑1 (MQ=255)
                                                            ccACAGGCGAGAGCAGTAAAATGGCGGTCAAAATGGTAATAGCCATTCAGGACATTATGAAATCTGCCAGGTGCCACATCAAAGGGAGACTTAGCAAGGTTCCGCCGATGACCGTTGCGACGGAGCAGAACCGCAAACACCAACTCGCTTTTGGGGTGTTCAGTCGAAAAAAGAAGAGATTGATTTCGGCGTTAATCTAGTTTGCGAAGATGAAGATGAAGGCAAACAAAATAACCCCGAGGGTAACAAAc                                                                                                                                                                                         >  2:49993/1‑251 (MQ=255)
                                                              acaGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTc                                                                                                                                                                                       <  1:21518/251‑1 (MQ=255)
                                                                  gCGAGAGCTGTAAAATGGCGGTCAAATTGGTAATGGCCATGCAGGCCATTAGGATATCTGCCAGTTGCCACATCAGCGGAAGACGTAGCAAGGTGCCGCCGATGACCGTTGCGAATGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGGAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCa                                                                                                                                                                                   <  2:116872/251‑1 (MQ=255)
                                                                  gCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGAGATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCa                                                                                                                                                                                   <  1:83169/251‑1 (MQ=255)
                                                                           gTAAAATGGCTGGCAAATTTGGAATATCCTGGCAGGCCAGTATGATATCCGCGAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGTGTGTGCAGGCGTAAAAAGAAGAGATTGCTGTCGGCGTAAATGTAGTTGGCAACGAGGGAGCTGAAGGCAGACATAATAACCACGAGGGTGACAAAATCAGCAcctgaggaa                                                                                                                                                                          <  2:162637/251‑8 (MQ=255)
                                                                                  ggCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCAtt                                                                                                                                                                    >  1:240974/1‑250 (MQ=255)
                                                                                                          gCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGGGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATa                                                                                                                                            <  1:365025/250‑1 (MQ=255)
                                                                                                            aGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCATAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGc                                                                                                                                         >  1:2346/1‑251 (MQ=255)
                                                                                                                                       acaTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGtt                                                                                                             >  1:351439/1‑251 (MQ=255)
                                                                                                                                                           aaGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTa                                                                                          >  1:105792/1‑250 (MQ=255)
                                                                                                                                                                                             tCCGCAAACACAAGATCGCTTTAAGGGTGTTCAGGCGTAATATGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCAATTAAAACCCGCATCGCCTTCTGGATAAGCTGAATCCATTCC‑AGCGGCTTGTAGATTGTGCCGGTAAACGCCAGTAATATCAGCATGCCGCtgtacgtacagcagaccacg                                                       >  2:45075/1‑232 (MQ=255)
                                                                                                                                                                                                              gCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaaacttcc                                      >  1:61594/1‑246 (MQ=255)
                                                                                                                                                                                                                           tCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCCGGGTGTCGATAAAAATGCCAATCATCTGGAc                          >  1:204960/1‑250 (MQ=255)
                                                                                                                                                                                                                                   aaaaaGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATc                                                                                     >  1:38202/1‑183 (MQ=255)
                                                                                                                                                                                                                                    aaaaGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTgc                >  1:15113/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gaagaGATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCt                                                                          >  1:367614/1‑189 (MQ=255)
                                                                                                                                                                                                                                         aagaGATTGTGTTCGGCGTAAAGTTAGTTGGCAACGATGGAGCGAAAGGAAAAAAAAAAAACCAGGAGGATAAAAAACCCAGCCCCCCAGAAACCCTTTAACCCCCGCTTCGCCTTCGGGAAAAGCTGAAT‑CCTTCCCAGGGGAATGTGGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGc           <  1:49993/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  ttttCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCgg  >  1:205465/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   tttCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGCTAAAAATGCCAATCATCTGGACAATCCCTTGCTCTGCCGGATGCgg  >  1:78388/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   tttCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTT‑CCGTACAGATTACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCgg  >  1:218889/1‑249 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   
AAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAAT‑CACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCC‑AGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGG  >  NC_000913/6601‑7092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑