Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,716	A→G	I269I (ATT→ATC)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,716	0	A	G	100.0%	135.9 / NA	44	I269I (ATT→ATC)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/13);  total (31/13)

GCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCC‑TTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTC  >  NC_000913/4629474‑4629956                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 gccccTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGGCtt                                                                                                                                                                                                                                          >  1:89590/4‑251 (MQ=255)               cgggaAGCTCAGGTTATCAATCAGCAGGCGATCACCGTAGGATTTACGTAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCCGACGTGCTTTACCTGTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGct                                                                                                                                                                                                                             <  1:140444/247‑1 (MQ=255)                 agaAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGcttct                                                                                                                                                                                                                          >  1:299106/1‑251 (MQ=255)                                ccatcaGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTAATCGCCCAGACGCGGTCCCGGTGGAATAAACAGTTCGTTGGTTTCGTTAAGTTTCGGATATTCGGTGCTGATCAGCTCTTCAAAGCGTGCCAGCCGTGATTTACATTTCGCCTGTAGGCCATTAAGT‑CCCTGACGTACCCCCTCCAGCTCTTTCAAGCTCGCATTACGCCGCGCCGCTTCTTGAGAAGATTcttgg                                                                                                                                                                                                           >  2:296367/3‑247 (MQ=255)                                     agcagGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                                                                                                                                                                                        >  1:312021/1‑249 (MQ=255)                                        aggcGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGAGCTTTCCCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCCACTTACGACCCGCCGCTTCTTGTGAAGCTTCCTggtccggtgg                                                                                                                                                                                                   >  2:179145/4‑242 (MQ=255)                                          gcGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                                                                                                                                                                                 >  1:70562/2‑251 (MQ=255)                                                      ggATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAGTAAACAGGTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCGAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAATTCCCTGACGTACCCCCTCAAGCTCTTTCTCGATCGACTTCCGCCGCGCCGCTTCTTGTGAAGATTCCTGCGCCAGGCGCTGATCTTTCTGCt                                                                                                                                                                                     >  2:216035/1‑251 (MQ=255)                                                            aCGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGCGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcc                                                                                                                                                                               >  1:321633/1‑251 (MQ=255)                                                                  gTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg                                                                                                                                                                         <  1:180523/251‑1 (MQ=255)                                                                      cTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                                                                                      >  1:2603/1‑250 (MQ=255)                                                                      cTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                                                                                      >  1:4973/1‑250 (MQ=255)                                                                       tGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCATCTTGTGACGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                                                                                      >  1:253082/1‑249 (MQ=255)                                                                        gACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGTTCTTTCTGCTCCCGCCAGGAGGagtagt                                                                                                                                                                   >  1:202957/1‑251 (MQ=255)                                                                                            ccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                                                                                                                                                                                         <  1:189051/193‑1 (MQ=255)                                                                                            ccAGACGCGGTCAAGGCGGAATAAACAGTTCGTTGGTTTCCTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGCCGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                                                                                                                                                                                         >  2:189051/1‑193 (MQ=255)                                                                                                 cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGATTAGTTGCCTTCCCACGGAATACCTTCAcc                                                                                                                                          <  1:320825/251‑1 (MQ=255)                                                                                                          ggTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGAACCCCCTCCAGCTCTTTCTCGATCGACTTACGACGCCCCGCTTCTTGTGAAACTTCCTGCGCCCGGCGCTGATCTCTCTGCTCCAGCCGAGAGGTGTGGTTGCCTTCCCTCGGCATAACTTACCCGCGGCCaa                                                                                                                                  >  2:82496/1‑250 (MQ=255)                                                                                                            tGGAATAAACAGTTCGTTGGTTTAGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATAACTTCACCGCGGTCAGGtt                                                                                                                               >  1:189295/1‑251 (MQ=255)                                                                                                                  aaaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcca                                                                                                                                                                              >  1:360159/1‑198 (MQ=255)                                                                                                                           gTTGGTTTCGTTACGTTTCTGATATTCGGGGCTGTTCAGCTATTCAAAGCGTGCCAGAAGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGAAGCGCCGCTTCTTGTGAAGCTTCCTGCGCAAGGCGCTGATCTTTCTGTTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGTGGTCAAGTTCGAGGATCCAGACTg                                                                                                                >  1:228579/1‑251 (MQ=255)                                                                                                                              ggTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCa                                                                                                              <  1:323311/250‑1 (MQ=255)                                                                                                                                          tttCTGATCTTCACTGCTGTTCAGCTCTTCAAAGCGTGCCCGTAGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCAACTCCAGCTCTTTCTCGCTCGACTTACGTCGCGCCGCTTCTTATGATGCTTCCTGCGCCAAGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCATTCCCACGGATGACCTTCACCGCGGACAAGTTCGCGGAACCAGCCTGCAACGtaaacgcggg                                                                                                 >  2:377042/1‑242 (MQ=255)                                                                                                                                                  atTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACg                                                                                          <  1:307323/250‑1 (MQ=255)                                                                                                                                                      ggTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTCTCGAGGAAGTAACGGTCgg                                                                                     >  1:355761/1‑250 (MQ=255)                                                                                                                                                               cagctcttcAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACTCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCg                                                                            <  1:330660/247‑1 (MQ=255)                                                                                                                                                                 gccctttaaaGCGTGCCAGACGTCCTTTACCTTTCGACTGGCGGCC‑TTTATTTCCCTGACGTACCCACCCCAGCTCTTGCTCGATCGTCTTACGTCGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGcc                                                                          <  2:2603/244‑1 (MQ=255)                                                                                                                                                                    cttcAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCcacc                                                                       >  1:369613/1‑250 (MQ=255)                                                                                                                                                                               gcCAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCATACTTACCCACGCCAGCTCTTTCTCGATCGACATACGTCTCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGACTTCCCACGGAATACCTTCACCGCGGCCAAGTTCGAGGATCCAGCCTGCAACGTTATCGATGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCtt                                                             <  2:60055/250‑1 (MQ=255)                                                                                                                                                                                ccAGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCg                                                           <  1:155267/251‑1 (MQ=255)                                                                                                                                                                                  aGACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCCCGGCATACCTTCACCGCGGTCCAGTTCGCGGATCCAGCCTGCAACGTTATCGCGGAAGTAACGGTCGTGGGTACTCGCCACCCCGGTGCCCTCGa                                                          >  2:150098/1‑250 (MQ=255)                                                                                                                                                                                   gACGTGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGa                                                          >  1:72629/1‑249 (MQ=255)                                                                                                                                                                                       tGCTTTACCTTTCGCCTGGCGGAC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTcc                                                                                                                                                                                                              >  2:160767/1‑97 (MQ=255)                                                                                                                                                                                       tGCTTTACCTTTCGACTGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTcc                                                                                                                                                                                                              <  1:160767/97‑1 (MQ=255)                                                                                                                                                                                                           ggCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATATTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGGGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTccagcc                                  >  1:226660/1‑249 (MQ=255)                                                                                                                                                                                                                     ttccctGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGAGCCCTCGAAGTCGTGCAAGAAACGTTCCAGCCAGGCCACGGAt                       >  2:349988/6‑251 (MQ=255)                                                                                                                                                                                                                      tccctGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGCTCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGAGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACggctt                      >  2:338524/5‑248 (MQ=255)                                                                                                                                                                                                                       ccctGACGTACACACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTc                     >  1:134982/4‑251 (MQ=255)                                                                                                                                                                                                                              gTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCCGGCCACGGATTCGGCATcc              >  1:248474/1‑251 (MQ=255)                                                                                                                                                                                                                               tACCCACTCCAGCTCTTTCTCGATCGAATTACGACGCGCTGCTTCTTGTGCAGATTCCTGCGACAGGCGCTAACCTTTCTGATCCAGCCTGGAGGAGTAGTTGCCTTCCCACGGAATCACTTCACCGCGGTCAAGCTCGAGGATCCAGCCTGCAACGTTATCGAGGACGTAAGAGTCGTGAGTAATCGCAACCACGGTACCCTCGCAGACGGGAAGGAAGCGTTCAAGCCGGGCCACGCATTCGGcccca              >  2:249593/1‑246 (MQ=255)                                                                                                                                                                                                                                      tCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGCAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGCATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTa                                                                                >  2:380877/1‑177 (MQ=255)                                                                                                                                                                                                                                      tCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTa                                                                                <  1:380877/177‑1 (MQ=255)                                                                                                                                                                                                                                        cAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGtggttgg    >  1:45584/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTc  <  1:296367/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 GCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCC‑TTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTC  >  NC_000913/4629474‑4629956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑