Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,701	T→C	E274E (GAA→GAG)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,701	0	T	C	100.0%	122.6 / NA	44	E274E (GAA→GAG)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/12);  total (32/12)

ATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAC‑TGACGGCC‑TTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCC  >  NC_000913/4629462‑4629944                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    atCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCttt                                                                                                                                                                                                                                          >  1:269742/1‑251 (MQ=255)   cccGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTtct                                                                                                                                                                                                                                        >  1:326104/1‑251 (MQ=255)    ccGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTtctc                                                                                                                                                                                                                                       <  1:140899/251‑1 (MQ=255)         gcttgccccTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACGGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACGTG‑CGACA‑TTTAGTTCCCTTACGTCCCCACTCCAGCTTTTtctagatgc                                                                                                                                                                                                                                  >  2:307323/8‑245 (MQ=255)             gccccTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGGCtt                                                                                                                                                                                                                              >  1:89590/4‑251 (MQ=255)                           cgggaAGCTCAGGTTATCAATCAGCAGGCGATCACCGTAGGATTTACGTAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCCGACGTGCTTTACCTGTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGct                                                                                                                                                                                                                 <  1:140444/247‑1 (MQ=255)                             agaAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGcttct                                                                                                                                                                                                              >  1:299106/1‑251 (MQ=255)                                            ccatcaGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTAATCGCCCAGACGCGGTCCCGGTGGAATAAACAGTTCGTTGGTTTCGTTAAGTTTCGGATATTCGGTGCTGATCAGCTCTTCAAAGCGTGCCAGCCGTGATTTACATTTCGCC‑TGTAGGCCATTAAGT‑CCCTGACGTACCCCCTCCAGCTCTTTCAAGCTCGCATTACGCCGCGCCGCTTCTTGAGAAGATTcttgg                                                                                                                                                                                               >  2:296367/3‑247 (MQ=255)                                                 agcagGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                                                                                                                                                                            >  1:312021/1‑249 (MQ=255)                                                    aggcGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGAGCTTTCCCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCCACTTACGACCCGCCGCTTCTTGTGAAGCTTCCTggtccggtgg                                                                                                                                                                                       >  2:179145/4‑242 (MQ=255)                                                      gcGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                                                                                                                                                                     >  1:70562/2‑251 (MQ=255)                                                                  ggATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAGTAAACAGGTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCGAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAATTCCCTGACGTACCCCCTCAAGCTCTTTCTCGATCGACTTCCGCCGCGCCGCTTCTTGTGAAGATTCCTGCGCCAGGCGCTGATCTTTCTGCt                                                                                                                                                                         >  2:216035/1‑251 (MQ=255)                                                                        aCGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGCGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcc                                                                                                                                                                   >  1:321633/1‑251 (MQ=255)                                                                              gTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg                                                                                                                                                             <  1:180523/251‑1 (MQ=255)                                                                                  cTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                                                                          >  1:2603/1‑250 (MQ=255)                                                                                  cTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                                                                          >  1:4973/1‑250 (MQ=255)                                                                                   tGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCATCTTGTGACGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                                                                          >  1:253082/1‑249 (MQ=255)                                                                                    gACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGTTCTTTCTGCTCCCGCCAGGAGGagtagt                                                                                                                                                       >  1:202957/1‑251 (MQ=255)                                                                                                        ccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                                                                                                                                                                             <  1:189051/193‑1 (MQ=255)                                                                                                        ccAGACGCGGTCAAGGCGGAATAAACAGTTCGTTGGTTTCCTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGCCGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                                                                                                                                                                             >  2:189051/1‑193 (MQ=255)                                                                                                             cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGATTAGTTGCCTTCCCACGGAATACCTTCAcc                                                                                                                              <  1:320825/251‑1 (MQ=255)                                                                                                                      ggTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGAACCCCCTCCAGCTCTTTCTCGATCGACTTACGACGCCCCGCTTCTTGTGAAACTTCCTGCGCCCGGCGCTGATCTCTCTGCTCCAGCCGAGAGGTGTGGTTGCCTTCCCTCGGCATAACTTACCCGCGGCCaa                                                                                                                      >  2:82496/1‑250 (MQ=255)                                                                                                                        tGGAATAAACAGTTCGTTGGTTTAGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATAACTTCACCGCGGTCAGGtt                                                                                                                   >  1:189295/1‑251 (MQ=255)                                                                                                                              aaaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcca                                                                                                                                                                  >  1:360159/1‑198 (MQ=255)                                                                                                                                       gTTGGTTTCGTTACGTTTCTGATATTCGGGGCTGTTCAGCTATTCAAAGCGTGCCAGAAGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGAAGCGCCGCTTCTTGTGAAGCTTCCTGCGCAAGGCGCTGATCTTTCTGTTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGTGGTCAAGTTCGAGGATCCAGACTg                                                                                                    >  1:228579/1‑251 (MQ=255)                                                                                                                                          ggTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCa                                                                                                  <  1:323311/250‑1 (MQ=255)                                                                                                                                                      tttCTGATCTTCACTGCTGTTCAGCTCTTCAAAGCGTGCCCGTAGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCAACTCCAGCTCTTTCTCGCTCGACTTACGTCGCGCCGCTTCTTATGATGCTTCCTGCGCCAAGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCATTCCCACGGATGACCTTCACCGCGGACAAGTTCGCGGAACCAGCCTGCAACGtaaacgcggg                                                                                     >  2:377042/1‑242 (MQ=255)                                                                                                                                                              atTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACg                                                                              <  1:307323/250‑1 (MQ=255)                                                                                                                                                                  ggTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTCTCGAGGAAGTAACGGTCgg                                                                         >  1:355761/1‑250 (MQ=255)                                                                                                                                                                           cagctcttcAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACTCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCg                                                                <  1:330660/247‑1 (MQ=255)                                                                                                                                                                             gccctttaaaGCGTGCCAGACGTCCTTTACCTTTCGAC‑TGGCGGCC‑TTTATTTCCCTGACGTACCCACCCCAGCTCTTGCTCGATCGTCTTACGTCGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGcc                                                              <  2:2603/244‑1 (MQ=255)                                                                                                                                                                                cttcAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCcacc                                                           >  1:369613/1‑250 (MQ=255)                                                                                                                                                                                           gcCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCATACTTACCCACGCCAGCTCTTTCTCGATCGACATACGTCTCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGACTTCCCACGGAATACCTTCACCGCGGCCAAGTTCGAGGATCCAGCCTGCAACGTTATCGATGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCtt                                                 <  2:60055/250‑1 (MQ=255)                                                                                                                                                                                            ccAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCg                                               <  1:155267/251‑1 (MQ=255)                                                                                                                                                                                              aGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCCCGGCATACCTTCACCGCGGTCCAGTTCGCGGATCCAGCCTGCAACGTTATCGCGGAAGTAACGGTCGTGGGTACTCGCCACCCCGGTGCCCTCGa                                              >  2:150098/1‑250 (MQ=255)                                                                                                                                                                                               gACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGa                                              >  1:72629/1‑249 (MQ=255)                                                                                                                                                                                                   tGCTTTACCTTTCGCC‑TGGCGGAC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTcc                                                                                                                                                                                                  >  2:160767/1‑97 (MQ=255)                                                                                                                                                                                                   tGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTcc                                                                                                                                                                                                  <  1:160767/97‑1 (MQ=255)                                                                                                                                                                                                                        ggCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATATTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGGGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTccagcc                      >  1:226660/1‑249 (MQ=255)                                                                                                                                                                                                                                  ttccctGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGAGCCCTCGAAGTCGTGCAAGAAACGTTCCAGCCAGGCCACGGAt           >  2:349988/6‑251 (MQ=255)                                                                                                                                                                                                                                   tccctGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGCTCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGAGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACggctt          >  2:338524/5‑248 (MQ=255)                                                                                                                                                                                                                                    ccctGACGTACACACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTc         >  1:134982/4‑251 (MQ=255)                                                                                                                                                                                                                                           gTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCCGGCCACGGATTCGGCATcc  >  1:248474/1‑251 (MQ=255)                                                                                                                                                                                                                                            tACCCACTCCAGCTCTTTCTCGATCGAATTACGACGCGCTGCTTCTTGTGCAGATTCCTGCGACAGGCGCTAACCTTTCTGATCCAGCCTGGAGGAGTAGTTGCCTTCCCACGGAATCACTTCACCGCGGTCAAGCTCGAGGATCCAGCCTGCAACGTTATCGAGGACGTAAGAGTCGTGAGTAATCGCAACCACGGTACCCTCGCAGACGGGAAGGAAGCGTTCAAGCCGGGCCACGCATTCGGcccca  >  2:249593/1‑246 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    ATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAC‑TGACGGCC‑TTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCC  >  NC_000913/4629462‑4629944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑