Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,647	C→T	A292A (GCG→GCA)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,647	0	C	T	100.0%	115.2 / NA	38	A292A (GCG→GCA)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/12);  total (26/12)

GAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAC‑TGACGGCC‑TTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACA  >  NC_000913/4629414‑4629887                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   gagaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTACAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTAAAAGCGTGCCAGACGTGCTTTAcc                                                                                                                                                                                                                                   >  1:238324/1‑251 (MQ=255)      cATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGACCCCGACGATTGCCCCTTGCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCg                                                                                                                                                                                                                              <  1:64881/251‑1 (MQ=255)         aCGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑t                                                                                                                                                                                                                          >  1:114863/1‑251 (MQ=255)          cGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑Tg                                                                                                                                                                                                                         <  1:383717/251‑1 (MQ=255)                      gATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGt                                                                                                                                                                                                            <  1:212583/251‑1 (MQ=255)                                              atgatCCCGACGCTTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTGGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGGGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGAGCTGTTCAGCTCTTCAAAGCGTGCCAGCAGTGCTTTACCTTTCGAC‑TGTCGGCC‑TTTAGTGCCCTGACGttccaaccacatctc                                                                                                                                                                                    >  2:110781/1‑237 (MQ=255)                                                 atCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCttt                                                                                                                                                                                 >  1:269742/1‑251 (MQ=255)                                                   cccGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTtct                                                                                                                                                                               >  1:326104/1‑251 (MQ=255)                                                    ccGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTtctc                                                                                                                                                                              <  1:140899/251‑1 (MQ=255)                                                         gcttgccccTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACGGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACGTG‑CGACA‑TTTAGTTCCCTTACGTCCCCACTCCAGCTTTTtctagatgc                                                                                                                                                                         >  2:307323/8‑245 (MQ=255)                                                             gccccTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGGCtt                                                                                                                                                                     >  1:89590/4‑251 (MQ=255)                                                                           cgggaAGCTCAGGTTATCAATCAGCAGGCGATCACCGTAGGATTTACGTAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCCGACGTGCTTTACCTGTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGct                                                                                                                                                        <  1:140444/247‑1 (MQ=255)                                                                             agaAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGcttct                                                                                                                                                     >  1:299106/1‑251 (MQ=255)                                                                                            ccatcaGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTAATCGCCCAGACGCGGTCCCGGTGGAATAAACAGTTCGTTGGTTTCGTTAAGTTTCGGATATTCGGTGCTGATCAGCTCTTCAAAGCGTGCCAGCCGTGATTTACATTTCGCC‑TGTAGGCCATTAAGT‑CCCTGACGTACCCCCTCCAGCTCTTTCAAGCTCGCATTACGCCGCGCCGCTTCTTGAGAAGATTcttgg                                                                                                                                      >  2:296367/3‑247 (MQ=255)                                                                                                 agcagGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                                                                                                                   >  1:312021/1‑249 (MQ=255)                                                                                                    aggcGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGAGCTTTCCCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCCACTTACGACCCGCCGCTTCTTGTGAAGCTTCCTggtccggtgg                                                                                                                              >  2:179145/4‑242 (MQ=255)                                                                                                      gcGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                                                                                                            >  1:70562/2‑251 (MQ=255)                                                                                                                  ggATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAGTAAACAGGTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCGAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAATTCCCTGACGTACCCCCTCAAGCTCTTTCTCGATCGACTTCCGCCGCGCCGCTTCTTGTGAAGATTCCTGCGCCAGGCGCTGATCTTTCTGCt                                                                                                                >  2:216035/1‑251 (MQ=255)                                                                                                                        aCGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGCGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcc                                                                                                          >  1:321633/1‑251 (MQ=255)                                                                                                                              gTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg                                                                                                    <  1:180523/251‑1 (MQ=255)                                                                                                                                  cTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                 >  1:4973/1‑250 (MQ=255)                                                                                                                                  cTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                 >  1:2603/1‑250 (MQ=255)                                                                                                                                   tGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCATCTTGTGACGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt                                                                                                 >  1:253082/1‑249 (MQ=255)                                                                                                                                    gACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGTTCTTTCTGCTCCCGCCAGGAGGagtagt                                                                                              >  1:202957/1‑251 (MQ=255)                                                                                                                                                        ccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                                                                                                                    <  1:189051/193‑1 (MQ=255)                                                                                                                                                        ccAGACGCGGTCAAGGCGGAATAAACAGTTCGTTGGTTTCCTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGCCGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                                                                                                                    >  2:189051/1‑193 (MQ=255)                                                                                                                                                             cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGATTAGTTGCCTTCCCACGGAATACCTTCAcc                                                                     <  1:320825/251‑1 (MQ=255)                                                                                                                                                                      ggTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGAACCCCCTCCAGCTCTTTCTCGATCGACTTACGACGCCCCGCTTCTTGTGAAACTTCCTGCGCCCGGCGCTGATCTCTCTGCTCCAGCCGAGAGGTGTGGTTGCCTTCCCTCGGCATAACTTACCCGCGGCCaa                                                             >  2:82496/1‑250 (MQ=255)                                                                                                                                                                        tGGAATAAACAGTTCGTTGGTTTAGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATAACTTCACCGCGGTCAGGtt                                                          >  1:189295/1‑251 (MQ=255)                                                                                                                                                                              aaaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcca                                                                                                         >  1:360159/1‑198 (MQ=255)                                                                                                                                                                                       gTTGGTTTCGTTACGTTTCTGATATTCGGGGCTGTTCAGCTATTCAAAGCGTGCCAGAAGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGAAGCGCCGCTTCTTGTGAAGCTTCCTGCGCAAGGCGCTGATCTTTCTGTTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGTGGTCAAGTTCGAGGATCCAGACTg                                           >  1:228579/1‑251 (MQ=255)                                                                                                                                                                                          ggTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCa                                         <  1:323311/250‑1 (MQ=255)                                                                                                                                                                                                      tttCTGATCTTCACTGCTGTTCAGCTCTTCAAAGCGTGCCCGTAGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCAACTCCAGCTCTTTCTCGCTCGACTTACGTCGCGCCGCTTCTTATGATGCTTCCTGCGCCAAGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCATTCCCACGGATGACCTTCACCGCGGACAAGTTCGCGGAACCAGCCTGCAACGtaaacgcggg                            >  2:377042/1‑242 (MQ=255)                                                                                                                                                                                                              atTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACg                     <  1:307323/250‑1 (MQ=255)                                                                                                                                                                                                                  ggTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTCTCGAGGAAGTAACGGTCgg                >  1:355761/1‑250 (MQ=255)                                                                                                                                                                                                                           cagctcttcAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACTCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCg       <  1:330660/247‑1 (MQ=255)                                                                                                                                                                                                                             gccctttaaaGCGTGCCAGACGTCCTTTACCTTTCGAC‑TGGCGGCC‑TTTATTTCCCTGACGTACCCACCCCAGCTCTTGCTCGATCGTCTTACGTCGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGcc     <  2:2603/244‑1 (MQ=255)                                                                                                                                                                                                                                cttcAAAGCGTGCCAGACGTGCTTTACCTTTCGAC‑TGGCGGCC‑TTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCcacc  >  1:369613/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   GAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAC‑TGACGGCC‑TTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACA  >  NC_000913/4629414‑4629887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑