Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,473	C→T	A350A (GCG→GCA)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,473	0	C	T	100.0%	79.9 / NA	28	A350A (GCG→GCA)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

GGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTC  >  NC_000913/4629245‑4629715                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   ggCGCGGCTTGGCATCTCGGTGTTGCCGGTCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGCCGATTGCCCCTTTCGGGTTCGACAAGc                                                                                                                                                                                                                              >  2:60507/1‑251 (MQ=255)           ggCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtcatc                                                                                                                                                                                                                    <  1:60507/251‑1 (MQ=255)                         gCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGAt                                                                                                                                                                                                      <  1:127821/251‑1 (MQ=255)                                           cAGCCCGCCGGAAACTTCTTCCCATACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTCCCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCTCCGTAGGATTTACGCa                                                                                                                                                                                    <  2:379901/251‑1 (MQ=255)                                                   cgGAAACGTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTg                                                                                                                                                                            <  2:88048/251‑1 (MQ=255)                                                                 cAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACttt                                                                                                                                                              >  1:60055/1‑251 (MQ=255)                                                                                             cACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAAt                                                                                                                                   >  1:275443/1‑250 (MQ=255)                                                                                              aCGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGACGCTCAGGTCATCAATAAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTAACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGGGGAATa                                                                                                                                  >  1:222529/1‑250 (MQ=255)                                                                                                      gATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTc                                                                                                                          <  1:158035/250‑1 (MQ=255)                                                                                                      gATCAACCGATGCAAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGCGATCATACGGAACAGGGTCGATTTACACGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCCGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGATGATGACTTCAAGCACTTTATCGCCCAGACGCGGTCAAGGTGGAATAAACAGTTCg                                                                                                                         >  2:188476/1‑251 (MQ=255)                                                                                                              gatgCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTc                                                                                                                 >  1:181916/4‑251 (MQ=255)                                                                                                                 gCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGtt                                                                                                              <  1:170391/251‑1 (MQ=255)                                                                                                                   cAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTAc                                                                                                            <  1:66881/251‑1 (MQ=255)                                                                                                                       ttcaccgtttcaccTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGAAGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGttt                                                                                                        >  1:372882/1‑251 (MQ=255)                                                                                                                       ttcaccgtttcaccGAAAGTGATGGTGCCGCTGTACGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGTCCAGACGCGGTCCAGGTGGAATAAACAGTTAGTTGGTTTCGTTACGtt                                                                                                         <  1:151621/250‑1 (MQ=255)                                                                                                                                          tGATGGTGCCGCTGTCCGGCTGTTCATGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGGCCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATAAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGCATTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTc                                                                                     >  1:123050/1‑251 (MQ=255)                                                                                                                                                  ccGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCTTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGctcttc                                                                             <  2:275443/251‑1 (MQ=255)                                                                                                                                                           ggCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCATGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAgcgtgc                                                                    <  1:235065/251‑4 (MQ=255)                                                                                                                                                                          gagaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTACAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTAAAAGCGTGCCAGACGTGCTTTAcc                                                     >  1:238324/1‑251 (MQ=255)                                                                                                                                                                          gaaaTCATACGGAACAGGGTCGATTTACCCGCGACGTTCGGACAGATGATACCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTGCGCAGGTTGCTGACTTCCAGCACTTTAACGCCCAGACGCGGTCGAGGTGGAATAAAAAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCaaacctggaccgacgcgctttatc                                                     >  2:155267/4‑230 (MQ=255)                                                                                                                                                                               cATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGACCCCGACGATTGCCCCTTGCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCg                                                <  1:64881/251‑1 (MQ=255)                                                                                                                                                                                  aCGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACt                                             >  1:114863/1‑251 (MQ=255)                                                                                                                                                                                   cGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTg                                            <  1:383717/251‑1 (MQ=255)                                                                                                                                                                                               gATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGt                                <  1:212583/251‑1 (MQ=255)                                                                                                                                                                                                                       atgatCCCGACGCTTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTGGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGGGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGAGCTGTTCAGCTCTTCAAAGCGTGCCAGCAGTGCTTTACCTTTCGACTGTCGGCCTTTAGTGCCCTGACGttccaaccacatctc        >  2:110781/1‑237 (MQ=255)                                                                                                                                                                                                                          atCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCttt     >  1:269742/1‑251 (MQ=255)                                                                                                                                                                                                                            cccGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTtct   >  1:326104/1‑251 (MQ=255)                                                                                                                                                                                                                             ccGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTtctc  <  1:140899/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   GGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTC  >  NC_000913/4629245‑4629715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑