Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 6,595 T→C P455P (CCA→CCG)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009136,5950TC88.5% 54.7 / 1.1 26P455P (CCA→CCG) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base C (12/11);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

CATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAG  >  NC_000913/6351‑6837
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
cATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAACGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTTGCAGGTGAAATAAGAATCATCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAATCTGGCGGCCGATATCCGGATAACGCAACGGATCa                                                                                                                                                                                                                                              <  1:376153/251‑1 (MQ=255)
     aTCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAacac                                                                                                                                                                                                                                         <  1:318170/251‑1 (MQ=255)
                        cTAACAGCTCCGGAAGCGTATAGCGCGGGGAGGGCTACGGGCTTTAGCAAACAAGCGATTTCGCGGGTGAAATAATCATCAGCATATCCAGGCCTGGCAGGAAATATATGCAGACTTTAGCAAAAAAAGACATTGAGGGGAGCGATAGTTGTGATTACTCCTGCGAAACATCATACCACGCGGCCGGAGATAGCTGGCGGCCGATATCCGGTTAACGCAGCGGAGCAAACCCCGAGCGCCAGCCGAggtg                                                                                                                                                                                                                       >  2:365025/1‑247 (MQ=255)
                          aaCAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTAc                                                                                                                                                                                                                     <  1:21978/250‑1 (MQ=255)
                          aaCAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACg                                                                                                                                                                                                                    >  1:116872/1‑251 (MQ=255)
                           aCAGCTC‑GGCAGCGTATAGCGCGTGGTGTTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCt                                                                                                                                                                                                                  >  1:94067/1‑251 (MQ=255)
                                                          cAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGa                                                                                                                                                                                     <  1:330596/250‑1 (MQ=255)
                                                             cGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGCGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAAcc                                                                                                                                                                                  >  1:162637/1‑250 (MQ=255)
                                                                                   ttCGCCGGAGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCAGACTTTAGCAAAAAAAACGACTGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATCTCCGGATAACGCAACGGATCACACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGTTAAGCACTGACAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGc                                                                                                                                                           >  2:179513/1‑251 (MQ=255)
                                                                                       cAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTc                                                                                                                                                       <  1:144405/251‑1 (MQ=255)
                                                                                                   gAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAAt                                                                                                                                            >  1:141842/1‑250 (MQ=255)
                                                                                                     aTCAGCATATCCAGTCCGTGCAGGAAATTTATGCCGCCTGTAGCAAAACATGAGAATGAGATGATCGATAGTTGTGATTCCTCCT‑‑GAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGCCCGCTATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCAGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGACCCACAGGCGAGTGCAGTACAATGTCGGGCAAATTGGTaaatgca                                                                                                                                        >  2:134606/1‑245 (MQ=255)
                                                                                                                     cTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTCAAATGGc                                                                                                                                                           >  2:54313/1‑217 (MQ=255)
                                                                                                                     cTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGc                                                                                                                                                           <  1:54313/217‑1 (MQ=255)
                                                                                                                       tGCAGGAAATTTATGCCGTATTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGTGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGa                                                                                                                        <  1:253278/250‑1 (MQ=255)
                                                                                                                               aTTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGGAATAGCCATGCAGGCCCTTATGATATCTGccc                                                                                                               >  1:9384/1‑250 (MQ=255)
                                                                                                                                                    aaaagagaatgagTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCCGCGGAAgac                                                                                          >  1:213361/5‑249 (MQ=255)
                                                                                                                                                                  tGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGACGAGAGCAGTAAAATGGCGGTCAAATTGGGAATAGCCCTGCAGGCCATTATGATATCTGCCAGTTGCCAAATCAGCGGAAGACTTAGCAAGGGgccg                                                                            >  1:314347/1‑251 (MQ=255)
                                                                                                                                                                    aTCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATAGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTtccgcc                                                                          >  1:108151/1‑251 (MQ=255)
                                                                                                                                                                                             aaaCATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAAAAGCAATGCAGGCCATTCTGATATCTGCCAGTTGCCACATCAGAGGAAAACTTAGCAAGGTGCCGCCGATGACCGTTGCgcaagggc                                                      >  1:219265/1‑239 (MQ=255)
                                                                                                                                                                                                    atcCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCCTGCAGGCCCTTATGATATCTGCCAGTTGCCACATCAACGGAAGACTTAGCAAGGAGCCGCCGATGACCGTTGCGAAGGGGCAGATCCGCAAac                                          >  1:370291/1‑251 (MQ=255)
                                                                                                                                                                                                                   agaAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAg                           <  1:166333/251‑1 (MQ=255)
                                                                                                                                                                                                                              ggccGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAgg                <  1:382790/249‑1 (MQ=255)
                                                                                                                                                                                                                                     atCCGGATAACGCAACGGATCAAACACCTGGCGCACTCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGATAGCAGTAAAATGGCGGTCAAATTGTTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTTCCACAt                                                                                                     <  2:275254/159‑1 (MQ=255)
                                                                                                                                                                                                                                     atCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCCTTATGATATCTGCCAGTTGCCACAt                                                                                                     >  1:275254/1‑159 (MQ=255)
                                                                                                                                                                                                                                             aaCGCAACGGATCAAACACCTGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATGGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAagag  <  1:375262/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
CATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAG  >  NC_000913/6351‑6837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑