Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,802	G→T	intergenic (+255/+53)	nadR → / ← ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,802	0	G	T	100.0%	53.2 / NA	18	intergenic (+255/+53)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/5);  total (13/5)

CAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAG‑TTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACC  >  NC_000913/4628564‑4629038                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              cAAAGGAGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAg                                                                                                                                                                                                                                  <  1:310660/250‑1 (MQ=255)          ggTATAAAATAAGTTTTTT‑GAGGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTAAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAg                                                                                                                                                                                                                        <  1:210425/251‑1 (MQ=255)                  aataagttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGCGACCCGCTACGAAATTGCGGCTACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGACCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATACGGCGTTc                                                                                                                                                                                                                  >  1:156731/7‑250 (MQ=255)                                           ccTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCAtt                                                                                                                                                                                        >  1:351445/1‑251 (MQ=255)                                                    aaGAGGCGGTCGAAATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTAAGAAATGGCGGAAACTAAGGTTTTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCaa                                                                                                                                                                               <  1:346790/251‑1 (MQ=255)                                                                                  aaaacCCTGCCCGGGTGACCCGCTACGAAATTGCGTATACAAAGGCGGTTGTAGGCCGGAAAAGGCTTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCa                                                                                                                                                 <  1:248167/251‑1 (MQ=255)                                                                                    aaaCCTGCCCGAGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGACTGATGCGTCGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACACCGTATTGCAATTTCTTGGATTT‑TAGGCCGGATTAGGCGTTCACGCCGACTCCGGCATTTTACGCATTACTTCGCAATACGCTTGTGCTTTCTACGCTTTGGcacaagt                                                                                                                                               >  2:306818/1‑245 (MQ=255)                                                                                       ccTGCACGGGTGACCCGCTACGAAATTGCGGATACTATGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCAATCGGTGCTCAATGCCTGATGCGACGATTACCGCGTCTTATCAGACCTGGAG‑ATTCACAACGGATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCATTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTcccgtacg                                                                                                                                            >  1:8296/1‑245 (MQ=255)                                                                                                                    ggATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGGGCGTTTCTTGTActc                                                                                                               >  1:58932/1‑251 (MQ=255)                                                                                                                         cTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGt                                                                                                          >  1:207791/1‑251 (MQ=255)                                                                                                                                                    ggtCACGCCGTAACCGCCATTCGATGCTCAATGCCTGATGCGACGCTTACCGCGTATTATCAGGCCT‑CAGGATTCACAACGTATTGGAATTTCT‑TGGTTTGTAGGCCGGCTAAACCCTTCACGCCGTAACCCGCATTTTACGCATTGCTTAGCAGTACGCTTGTCCTTCCTACGCCTCGGCTCCAGTGCGACGGCGACCAGAGTGGGTTTCTTGCACTATTCGTACTCgtacagatcagatacttagcact                                                                               >  2:128954/3‑229 (MQ=255)                                                                                                                                                       cACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCaa                                                                            <  1:58808/251‑1 (MQ=255)                                                                                                                                                                  cGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTc                                                                  >  1:44447/1‑250 (MQ=255)                                                                                                                                                                      aTTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCt                                                             >  1:116194/1‑251 (MQ=255)                                                                                                                                                                                                gCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGAt                                    >  1:201956/1‑250 (MQ=255)                                                                                                                                                                                                gCTTACCGCGTCTTATCAGGCCTGGAG‑ATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGAt                                    >  1:186645/1‑250 (MQ=255)                                                                                                                                                                                                                           gattcaCAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTACAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGTAACCAACGGTCGTGCgg         >  2:361071/6‑250 (MQ=255)                                                                                                                                                                                                                                  aaCGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAAcc  >  2:86123/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              CAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAG‑TTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACC  >  NC_000913/4628564‑4629038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑