| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,628,442 | C→G | L376L (CTC→CTG) | nadR → | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,628,442 | 0 | C | G | 86.2% | 71.5 / 3.8 | 29 | L376L (CTC→CTG) | nadR | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Reads supporting (aligned to +/- strand): ref base C (3/1); new base G (18/7); total (21/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATG > NC_000913/4628202‑4628683 | ttttGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCTGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAagagcac > 2:149606/1‑247 (MQ=255)ttttGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGCAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAAc > 1:236158/1‑250 (MQ=255) gCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATACGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCg > 1:360027/1‑251 (MQ=255) tGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGCATACCGTTTCGATCTGGTGATCCTGCTGGAGCACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAag > 1:359661/1‑249 (MQ=255) gacactgATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGACGAGGACGATTatg > 1:87440/7‑248 (MQ=255) aTTTTGTCAATACACAGGAGTTCTGCAAAAAGTACGAAGCGCGTGAGCATCCGTTCGTACAGGCGTTGCTTGATGAATACCGTTGCGATATGGAGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAACGAGTTCCAGAACTTGCTGGTGGAGACGTTGGGCGAGAACAATATCGTATTCGTGCGGGATGaatcgaatgcttagggcagccgt > 2:24144/1‑230 (MQ=255) tcactacccAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGaa < 1:198602/201‑1 (MQ=255) tcactacccAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGGTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGaa > 2:198602/9‑209 (MQ=255) ccAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCTGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTTCGACAGTCGTTTCctgcgctgcg < 1:149606/250‑1 (MQ=255) agcATCCGTTCGTACAGGCGTTGATTGCTGCATAACGTTTCGATCTGGTGATCATGCTGAAGAACAACACGCCGTGGGTGGCGGATGGTTTACGAAGCCTCGGAAGTTCGGTGGGTCGCAAAGAGTTCCAGAACTTGATGGTGGAGATGCTCGAAGAGAACCATATCGACTTCGAGCGGGTTGAAGAGGCCGATTATAACAGCCGTTTCCTGCGCAGCGTGGAGCTGGTGCGGGGGATGATGGGGGAGcc > 1:244292/3‑249 (MQ=255) gTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAAc > 1:349963/1‑251 (MQ=255) caggggttgagtgatgaATCCGGTGTCGATCTGGTGGTCGTGCTGGAGAACAACACGCCTTGGGTGGCGGATGGGTTACGCAGCCTCGGCCGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATTCTGGAAGAGAACAATATCGAATTCGGGCGGGTTGAAGAGGACGATTATGACAGCCGTTGCCTTCGCTGCGGGGAGCTGTTGCGGGAGATGATGGGGGAGCATAGATAACCGcgatg < 2:183060/240‑1 (MQ=255) cAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCTAAGAGTTCCAGACCTTGCTGGTGGAGATGCTGGAAGAGCACAATATCGACTTCGTGCGGGTTGCAGAGGACGATTATGACAGCCGTTTCCTGCGATGCGTGGCGCGGGTGCGGGGGTTGATGGGGGacctgagtcaaccgcgatg > 2:210425/1‑233 (MQ=255) aGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGCGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCGAGAACTTGCTGGTGGCGATTCTGGAAGTGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGAAAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGGGGGAGATGGTGGGGGAGCAGAGATAAcagagatg > 2:310660/1‑243 (MQ=255) gttgattgatGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAAcg < 1:181037/249‑2 (MQ=255) tttCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGacg < 1:215194/155‑3 (MQ=255) tttCGATCTGGTGATCCTGCTGGAGAAAAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTAGGTGGATCGCAAAGAGTTCCAGAACATGCTGGCGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGacg > 2:215194/1‑153 (MQ=255) cTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATCACCGCGCTGAAACGGCTCAAAGGCGAGGAATAAAAta > 1:351594/1‑248 (MQ=255) aTCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGtttttt > 1:359247/1‑251 (MQ=255) ccacacGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGAGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGCTTATGAAAGCGGTTTCCTGCGCTGCGTGGGGCTGGTGCGGGAGATGATGGCGGAGCAGAGATAACCGCGATGACACGGCTCAAAGGCGTGGGAAAAAAT‑ACGTTTTTTGATGAGTTGaaacc > 2:86074/3‑249 (MQ=255) aacaCGCCGTGGGTGGCGGATGGATTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGGTTTTT‑GATGAGTTGAAAAcc > 1:154223/1‑251 (MQ=255) ccGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAACCCTCTCTg > 1:362426/1‑251 (MQ=255) ggTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATaaa < 1:119127/204‑1 (MQ=255) ggTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGAATaaa > 2:119127/1‑204 (MQ=255) aGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACa > 1:4150/1‑250 (MQ=255) ggATCGCAAAGAGTTCCAGCACATGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGCGGACGATTATGACAGCCGTTTCATGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGTGATAACCGCGTTGAAACGGCTCAAAGGCGAGGAATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCACTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCtcc > 2:248167/1‑249 (MQ=255) cGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATGTCGAATTCGTGCGGGTTGAAGAGGACGATGATGACAGCCGTTTACTGCGCTGCGTTGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCCCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACgg < 1:24144/251‑1 (MQ=255) cAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGctac < 1:123736/251‑4 (MQ=255) ggAGATGCTCGAAGAGATCAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGCACTGGTGCGGGAGATGATGTGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATg > 1:142978/1‑251 (MQ=255) | TTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATG > NC_000913/4628202‑4628683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |