Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,213	G→A	V434V (GTG→GTA)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,213	0	G	A	94.6%	105.5 / ‑5.4	37	V434V (GTG→GTA)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (11/24);  total (11/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑ATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAA  >  NC_000913/4626970‑4627454                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   ggctgATCATGATGTGTTGGTGACCGTGTTCGGCGGCTGGAGGGTACACGAAACCAGCGCCGTCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGCCAGACCGCTGCCCCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGACCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTACCGaaa                                                                                                                                                                                                                                              <  2:301318/246‑1 (MQ=255) ggCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGaaaa                                                                                                                                                                                                                                             <  1:301528/251‑1 (MQ=255)     gATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAgc                                                                                                                                                                                                                                         >  1:379366/1‑251 (MQ=255)       tCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAgcgc                                                                                                                                                                                                                                       >  1:188408/1‑251 (MQ=255)                   tgtgAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATg                                                                                                                                                                                                                            <  1:151472/250‑1 (MQ=255)                                    gcgTGAAGGTAACTGAACTCAGCGCCGCCTTTGCGTTACTGCTGGCGATGTTGTCCAGCCTGCGTGACAGCCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTGCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGTATGCAGATTTTTGGCGTTaaa                                                                                                                                                                                                          <  2:129337/251‑1 (MQ=255)                                     ggTGAAGGCAACCGAACACAGCGCCGCCTTTGCGTGTCTGCTTGCGATGGTTTCCAGCCTTCGTGACATACCGCTGACACAGGATCTGGGGGTGTTTGGGGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaaa                                                                                                                                                                                                         <  2:300843/250‑1 (MQ=255)                                            gTAACCGAAACCAGCGCCGATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACtctc                                                                                                                                                                                                  <  2:191799/251‑1 (MQ=255)                                                   aaaCCAGCGCCGCCTTAGTGTTACTGTTGGCGATGGTGTCCAGCCTGCGTGACAGACCGCTGCCACAGGATTTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGCATTTCAGAAGCGGCGAAACTCGGTTTTCGCCGGGCGATTGTGCCGGCGGCTAACTTGCCTAAAAAAGTGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcgcg                                                                                                                                                                                           <  1:205088/251‑1 (MQ=255)                                                              gACTTTGCGTTACTGCTGGCGATGGTTTCCAGCCTTCGTGACAGGCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGtt                                                                                                                                                                                <  1:312889/251‑1 (MQ=255)                                                                      gTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACt                                                                                                                                                                        <  1:72450/251‑1 (MQ=255)                                                                       ttACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAACACTCTCCGAAGAGCTTAGCGTGTGCGACGACtt                                                                                                                                                                       >  2:51748/1‑251 (MQ=255)                                                                                        ttCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTTTAATGAG‑GTATACgg                                                                                                                                                      >  1:373951/1‑250 (MQ=255)                                                                                           cAGCCTGCGTGACAGACCGCGGCCACAGGATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACggaggg                                                                                                                                                  <  2:77316/251‑1 (MQ=255)                                                                                                 gcgtgACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGGGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGGAGGGATGCAGGTTTTTGGTGTTAAAGAACTCTCCGTCGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GCATCCGGAGGGAGatat                                                                                                                                            >  1:177934/5‑251 (MQ=255)                                                                                                  cgcgACAGCCCGCGGCCGCAGGATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCACTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGTTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGATTAGCGTGTTCGACGACTTATAAGGAG‑ATATACGGAGGGAGATATg                                                                                                                                           <  2:251894/251‑1 (MQ=255)                                                                                                      aCAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATgtcgt                                                                                                                                       <  1:65750/251‑1 (MQ=255)                                                                                                        tgACCGCTGCCACAGGATCTGGTGGAGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGGAACCCGGTTTTCGCCGGGCGCTATTTCCGGCGGCTAAAGGACCGAAAACAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTTTCCGACGCGCTTAACGTGTTAGACGCCTTATAATGAGGAT‑TACGGGGGGAGATATgtcgtct                                                                                                                                     >  2:341315/2‑250 (MQ=255)                                                                                                          aCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCAtt                                                                                                                                   <  1:201289/251‑1 (MQ=255)                                                                                                              cTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGGAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGCGATATGTCGTCATTTGAt                                                                                                                               >  2:326865/1‑251 (MQ=255)                                                                                                                      ggATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGTGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa                                                                                                                       <  1:68002/251‑1 (MQ=255)                                                                                                                               tgtgtTTGGGGAAGTCGGGCTGGCAGGGGAGATCCGCGCGGTGCCCAGCGGTCATGAACGAAGTGCAGAAGCGGCGAAACACGGTTTTCGGCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCTGAATGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAAGACGGAGGGAGATATGTCGTCATTTGATTTCCTGAAAACTGCCa                                                                                                               <  2:24155/249‑1 (MQ=255)                                                                                                                               ggtgTTAGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCCGCGGTAAGGGACGAATTTCAGAAGAGGCGAAACACGGGTTTCGCCGGGCGATTGATCCGGCGGCTAAAGCACCGAAAAAAGCGCCGAAAGGGATGAAAATTTTTGGCGTTAAAAACCTCTCCAAAGAGATTAGCGTGTTCGCAGACTTATAATGAG‑GTACACGGGGGGGGAGATGTAGTCATTTGTTTACCTGAAAAATGCCAt                                                                                                              >  2:246524/1‑251 (MQ=255)                                                                                                                                           aGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAggg                                                                                                  <  1:326865/251‑1 (MQ=255)                                                                                                                                              cGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCt                                                                                                <  1:15844/250‑1 (MQ=255)                                                                                                                                                ggCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCa                                                                                             <  1:341315/251‑1 (MQ=255)                                                                                                                                                        ggggAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAcag                                                                                     <  1:106596/251‑1 (MQ=255)                                                                                                                                                              aTCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGtg                                                                               <  1:334276/251‑2 (MQ=255)                                                                                                                                                                    ccGGTGCCCAGCGTTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTAGGCGTTAAAAAAATCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATAAGGAGTGAGATAGGTCGTCATTTGATTACCTGAACACTGCCATCAAGCAAATGGACTGCAAGCTACAGCAGGTGGCTGAt                                                                         >  2:207595/1‑251 (MQ=255)                                                                                                                                                                        ggCCCGGCGGTCAGGAACGAATTTCAGCAGCGGCGAAAAACGGTGTTCGCCGGGGGATGGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTGTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAAGACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCa                                                                     <  2:188408/250‑1 (MQ=255)                                                                                                                                                                          cccAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGc                                                                   >  1:350511/1‑251 (MQ=255)                                                                                                                                                                             aGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGTGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGt                                                                <  1:371700/251‑1 (MQ=255)                                                                                                                                                                                            atttcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGTGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTAt                                                 <  1:207164/245‑1 (MQ=255)                                                                                                                                                                                                     gcggcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg                                        <  1:242210/251‑1 (MQ=255)                                                                                                                                                                                                          gAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACt                                   <  1:246524/251‑1 (MQ=255)                                                                                                                                                                                                           aaaCACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTg                                  >  1:252518/1‑251 (MQ=255)                                                                                                                                                                                                                                           ggcTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACGCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGTGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaaaa  <  1:193848/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   GGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑ATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAA  >  NC_000913/4626970‑4627454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑