Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,162	T→A	S417S (TCT→TCA)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,162	0	T	A	97.4%	111.1 / ‑3.9	38	S417S (TCT→TCA)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (13/24);  total (13/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

AAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑ATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGT  >  NC_000913/4626916‑4627392                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        aaaCCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAg                                                                                                                                                                                                                                     <  1:265240/251‑1 (MQ=255)       tCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGTTGCGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACACGCGCCGACTTCGCGTTACTGCTGGCGCTGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGTTCTGGCGGTGTTTGGTGAAGTCGGGCTGGCAGGGCAGATCCGCCCGGTGCCCCGTGGCCAGGCACGCATTTCAGAAgcggcg                                                                                                                                                                                                                                >  1:54870/1‑250 (MQ=255)                ccTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGtt                                                                                                                                                                                                                      >  1:191799/1‑251 (MQ=255)                          gtcttGCACCGTCACGGTGGTCGGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGGGAA‑GGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGTCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCgg                                                                                                                                                                                                              <  1:350029/246‑1 (MQ=255)                                                 gccatgggacggtcggattggttttGTGAACGTGGTTGGCGG‑ATGAATGGTAACCAAATCGAGGCCCGGCTTGGCGTTAGTGCGGGCAATGGTTTCCGGCCTGCGGTCCAGTCCGCTTTCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCCGGGAGGGGAGATCCGCCCGGTGCCCAGCGGTGAGGAACGAATTTCAGAAGCGGCGAAACACCGTTTTCGCCGGGTGATTGTTCCGGCGGCTAACGtac                                                                                                                                                                                     <  2:3608/229‑3 (MQ=255)                                                       ggctgATCATGATGTGTTGGTGACCGTGTTCGGCGGCTGGAG‑GGTACACGAAACCAGCGCCGTCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGCCAGACCGCTGCCCCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGACCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTACCGaaa                                                                                                                                                                                <  2:301318/246‑1 (MQ=255)                                                       ggCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGaaaa                                                                                                                                                                               <  1:301528/251‑1 (MQ=255)                                                           gATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAgc                                                                                                                                                                           >  1:379366/1‑251 (MQ=255)                                                             tCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAgcgc                                                                                                                                                                         >  1:188408/1‑251 (MQ=255)                                                                     tgtTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCCGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCAACAGGATCTGGGGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAAATCCGCCCGGAGCCCAGCGGTCAGGCACGACTTTCAGAAGCGGCGAAACACGGTTTTCGCCGGACGATTGTACCGGCGGCTaaagaacagaaaaatgcgcaggacggg                                                                                                                                                                 >  1:307450/1‑226 (MQ=255)                                                                         tgtgAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATg                                                                                                                                                              <  1:151472/250‑1 (MQ=255)                                                                                          gcgTGAA‑GGTAACTGAACTCAGCGCCGCCTTTGCGTTACTGCTGGCGATGTTGTCCAGCCTGCGTGACAGCCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTGCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGTATGCAGATTTTTGGCGTTaaa                                                                                                                                            <  2:129337/251‑1 (MQ=255)                                                                                           ggTGAA‑GGCAACCGAACACAGCGCCGCCTTTGCGTGTCTGCTTGCGATGGTTTCCAGCCTTCGTGACATACCGCTGACACAGGATCTGGGGGTGTTTGGGGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaaa                                                                                                                                           <  2:300843/250‑1 (MQ=255)                                                                                                   gTAACCGAAACCAGCGCCGATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACtctc                                                                                                                                    <  2:191799/251‑1 (MQ=255)                                                                                                          aaaCCAGCGCCGCCTTAGTGTTACTGTTGGCGATGGTGTCCAGCCTGCGTGACAGACCGCTGCCACAGGATTTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGCATTTCAGAAGCGGCGAAACTCGGTTTTCGCCGGGCGATTGTGCCGGCGGCTAACTTGCCTAAAAAAGTGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcgcg                                                                                                                             <  1:205088/251‑1 (MQ=255)                                                                                                                     gACTTTGCGTTACTGCTGGCGATGGTTTCCAGCCTTCGTGACAGGCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGtt                                                                                                                  <  1:312889/251‑1 (MQ=255)                                                                                                                             gTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACt                                                                                                          <  1:72450/251‑1 (MQ=255)                                                                                                                              ttACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAACACTCTCCGAAGAGCTTAGCGTGTGCGACGACtt                                                                                                         >  2:51748/1‑251 (MQ=255)                                                                                                                                               ttCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTTTAATGAG‑GTATACgg                                                                                        >  1:373951/1‑250 (MQ=255)                                                                                                                                                  cAGCCTGCGTGACAGACCGCGGCCACAGGATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACggaggg                                                                                    <  2:77316/251‑1 (MQ=255)                                                                                                                                                        gcgtgACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGGGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGGAGGGATGCAGGTTTTTGGTGTTAAAGAACTCTCCGTCGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GCATCCGGAGGGAGatat                                                                              >  1:177934/5‑251 (MQ=255)                                                                                                                                                         cgcgACAGCCCGCGGCCGCAGGATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCACTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGTTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGATTAGCGTGTTCGACGACTTATAAGGAG‑ATATACGGAGGGAGATATg                                                                             <  2:251894/251‑1 (MQ=255)                                                                                                                                                             aCAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATgtcgt                                                                         <  1:65750/251‑1 (MQ=255)                                                                                                                                                               tgACCGCTGCCACAGGATCTGGTGGAGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGGAACCCGGTTTTCGCCGGGCGCTATTTCCGGCGGCTAAAGGACCGAAAACAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTTTCCGACGCGCTTAACGTGTTAGACGCCTTATAATGAGGAT‑TACGGGGGGAGATATgtcgtct                                                                       >  2:341315/2‑250 (MQ=255)                                                                                                                                                                 aCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCAtt                                                                     <  1:201289/251‑1 (MQ=255)                                                                                                                                                                     cTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGGAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGCGATATGTCGTCATTTGAt                                                                 >  2:326865/1‑251 (MQ=255)                                                                                                                                                                             ggATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGTGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa                                                         <  1:68002/251‑1 (MQ=255)                                                                                                                                                                                      tgtgtTTGGGGAAGTCGGGCTGGCAGGGGAGATCCGCGCGGTGCCCAGCGGTCATGAACGAAGTGCAGAAGCGGCGAAACACGGTTTTCGGCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCTGAATGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAAGACGGAGGGAGATATGTCGTCATTTGATTTCCTGAAAACTGCCa                                                 <  2:24155/249‑1 (MQ=255)                                                                                                                                                                                      ggtgTTAGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCCGCGGTAAGGGACGAATTTCAGAAGAGGCGAAACACGGGTTTCGCCGGGCGATTGATCCGGCGGCTAAAGCACCGAAAAAAGCGCCGAAAGGGATGAAAATTTTTGGCGTTAAAAACCTCTCCAAAGAGATTAGCGTGTTCGCAGACTTATAATGAG‑GTACACGGGGGGGGAGATGTAGTCATTTGTTTACCTGAAAAATGCCAt                                                >  2:246524/1‑251 (MQ=255)                                                                                                                                                                                                  aGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAggg                                    <  1:326865/251‑1 (MQ=255)                                                                                                                                                                                                     cGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCt                                  <  1:15844/250‑1 (MQ=255)                                                                                                                                                                                                       ggCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCa                               <  1:341315/251‑1 (MQ=255)                                                                                                                                                                                                               ggggAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAcag                       <  1:106596/251‑1 (MQ=255)                                                                                                                                                                                                                     aTCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGtg                 <  1:334276/251‑2 (MQ=255)                                                                                                                                                                                                                           ccGGTGCCCAGCGTTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTAGGCGTTAAAAAAATCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATAAGGAGTGAGATAGGTCGTCATTTGATTACCTGAACACTGCCATCAAGCAAATGGACTGCAAGCTACAGCAGGTGGCTGAt           >  2:207595/1‑251 (MQ=255)                                                                                                                                                                                                                               ggCCCGGCGGTCAGGAACGAATTTCAGCAGCGGCGAAAAACGGTGTTCGCCGGGGGATGGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTGTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAAGACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCa       <  2:188408/250‑1 (MQ=255)                                                                                                                                                                                                                                 cccAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGc     >  1:350511/1‑251 (MQ=255)                                                                                                                                                                                                                                    aGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGTGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGt  <  1:371700/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        AAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAA‑GGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑ATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGT  >  NC_000913/4626916‑4627392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑