Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,670	C→T	T253T (ACC→ACT)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,670	0	C	T	94.6%	98.6 / NA	38	T253T (ACC→ACT)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (20/15);  minor base G (0/2);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

AATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCA‑GTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGG  >  NC_000913/4626436‑4626905                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             cagtgtctctaTCCAGGTTAGGCATATGGCGGGTGTGCAGTCATGGCCTGGCAGCGGGGCGCAGGTGCGTGAAACGGTTGCGTATCTGACGCGCTTCGCCAAAACGCGCTGTGTGGCGAA‑GTGCATGGTTGGACACGTACCCAAGGATGGCTCGCTGGCTGGCTCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGAGGGTGATTCCGACTCCCGTTTTCGCACTTTACGCTGTCATaaa                                                                                                                                                                                                                               <  2:235600/240‑1 (MQ=255)          gcaccaggtagtGCATATGTCGGATGTCCAGTCTTCGCCGGGCAGCGGGGCGCAGGTTTGGGAACCGGCGGCTTATCTGACGCGCTGCGCCAAAACGCGCGTTGTGGCGATTGT‑CATGGTTGTACACGTATCCAAAGATGGCTAGCTGGCGGTCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCGTTTGGATGTCGATGCCGACTCCCGTTTGCTCACTTTACGCAGCCATAAAAACCGTTTCg                                                                                                                                                                                                                     <  2:377895/240‑1 (MQ=255)                  tGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGGCTGTTCGGTGCTTTTGGGTGGCGATGCCCACTCCCGTTTTCGCACTTTACGCAACCATAAAAACCGCTTCGGCGCGGTg                                                                                                                                                                                                             >  1:174780/1‑251 (MQ=255)                             gCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTggg                                                                                                                                                                                                  <  1:226019/251‑1 (MQ=255)                               ggATGTTCTGTCACCGCCTGGCTGCTTGGGGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCg                                                                                                                                                                                                <  2:361604/251‑1 (MQ=255)                                     aCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCACGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcg                                                                                                                                                                                          <  1:315239/251‑1 (MQ=255)                                                  ggcaGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACa                                                                                                                                                                             <  1:165117/248‑1 (MQ=255)                                                                            ggcggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGAGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCtt                                                                                                                                                   >  1:301318/1‑251 (MQ=255)                                                                                      ctGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTACCCAAAGATGGATCGCTGGCGGGCCCTAAAGTTCTGGAACACTGAATCGACTGTTCGGTGCTTTTGGGTGGCGATACCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCAATTCGGCGCGGTGAAAGAGCCAGGCGTCTTAGCGATGACCGAACAGGGGGTGGGGGAAATCCGCACCCCTTCAGCAAtttt                                                                                                                                         >  2:362152/2‑251 (MQ=255)                                                                                        gacgcGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCt                                                                                                                                       >  1:53149/5‑251 (MQ=255)                                                                                                tCGCCAAAACTCGCGTTGTTGCGATTGT‑CATGGTTGGATACGTAACCAAATATGGCTTGCTTGGGGGCCCTAAATTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGTCTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCTGCGCGGTGAATGAGCTGGTCGGCTTCGCGATGACCGAACAGGGGCTGCGTTAAGTCAGTAACCCTTCGGCAATTTTCTTAAGTcg                                                                                                                                <  2:334434/250‑1 (MQ=255)                                                                                                         cgcgcgGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGCCTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGa                                                                                                                       >  1:214383/1‑250 (MQ=255)                                                                                                               gtgtGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGCCTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGAAGATGAAGTGAcc                                                                                                                >  1:47472/1‑251 (MQ=255)                                                                                                                   ggCGATTGT‑CATGGTTGGACATGTAACCAAAGATGGCACGCTGGTGGGCCCGAAAGTGCAGGAACACTTGATCGACTGTGCGGTTCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACGTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGAGTCTTCGCGATGACCGAACAGGGGATGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGTAGTTACCtac                                                                                                             <  2:171989/250‑3 (MQ=255)                                                                                                                             cATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAATGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGACGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCTCGGCGATGAAGTGACCTCCGGTAGCTCA‑g                                                                                                  <  1:113502/251‑1 (MQ=255)                                                                                                                                ggttggacACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGGCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTg                                                                                                <  1:164634/243‑1 (MQ=255)                                                                                                                                 gttggacACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGAt                                                                                              <  1:318914/245‑1 (MQ=255)                                                                                                                                       cACGTCACCAAAGATGGCTCGCTGGCGGGCCCTAAAGGGCTGGAACAATGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGCCTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGACCAGGGGCTGCGTGGCGTCAGCAACCCTTCGGCAAGTTTCTTAAAGCTGGGAGATGAAGTGACCACCggccgccccgtgatgggggt                                                                                         >  2:278325/1‑233 (MQ=255)                                                                                                                                        aCGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATg                                                                                                                        >  1:372751/1‑219 (MQ=255)                                                                                                                                          gTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGGCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCggcaactccgtgttggaggtgtg                                                                                      >  1:252221/1‑230 (MQ=255)                                                                                                                                                            ggggcgggcctttaaGTTCTGGAACACTGTATCGTCTGTTCGGTGCGTTTGCATGGCGATGGCGACTCCCGTTTTCGCACTGTACTCAGCCATAAAAACCGATTCTGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCGTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGAACTCCGGCAGCTCC‑GTGATGGTGGGGTGGTAAGGAAcgagttcgtt                                                                   <  2:305858/238‑9 (MQ=255)                                                                                                                                                                    cccgaagggccTGGACCACTGTAGCGCCTGTTCGGTGCTTTGGGATGGCGTTGCCGGCTCCCTTTTTTGGACGTGGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCA‑GTGATGGTGGTATGGGAAGGAACGCGTCCACTGCtggtgg                                                           <  2:115983/241‑1 (MQ=255)                                                                                                                                                                       taaaGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTTAAGTCAGCAACCCTTCg                                                                                                                                                 <  2:351670/162‑1 (MQ=255)                                                                                                                                                                       taaaGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCg                                                                                                                                                 >  1:351670/2‑163 (MQ=255)                                                                                                                                                                              cTGGAACACTGAATCGACTGTTCGGTGCTTTTGGCTGGCGATGCCGACTCCCGTATTCGCACTGTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGTCGTCTTCGCGCCGACAGCACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGACGTGACCTCCGGCAGCTCC‑GGGATGGTGGTGTGGGAGGGTACGCGTcagcagctggtgtcgttcatggc                                                 >  2:65750/1‑229 (MQ=255)                                                                                                                                                                                ggAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGgcgc                                               >  1:227917/1‑251 (MQ=255)                                                                                                                                                                                     acTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCGGCCATAAAAACCGATTCGGCTCGGTGAATGCGATGGGCGTCTTCGCGATGACCGCACAGAGGCTGCGTGACGTCATCAACCCTTCGGCCATTTTCTTCCGTCTCGGCGATGAAGTGACATCCGGCAGCTCC‑ATAATGGTGGTGTGGGAAGGAACGCACACGTTGCTGGTGGAGATTCAGGCGCAGGTc                                          >  2:301528/1‑251 (MQ=255)                                                                                                                                                                                           tCGACTGTTCGGTGCTTTTGGCTGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGTGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCTATTTTCTTAAGTCGCGGCGATGCATTGACCTCCGGCAGCTAC‑GTGATGGTGGAGTGGGAAGGAACGCAGCCGGTGCTGGGGGAGATTCAGgcgggggccgagcat                                    >  2:17380/1‑239 (MQ=255)                                                                                                                                                                                            cGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAc                                    <  1:212567/250‑1 (MQ=255)                                                                                                                                                                                               cTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAACAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGGTGAAGTGACCTCCGGCAG‑ACACGTGATGGTGGTGTGGGAAGGGACGCGACCGTTGCTGGGGGAGATGCAGGAGCTGGTAGATAACTCgg                                >  2:314535/1‑250 (MQ=255)                                                                                                                                                                                                    cGGTGCTTTTGGATGGCGGTGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCGTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCgatgat                            <  2:198132/250‑1 (MQ=255)                                                                                                                                                                                                     ggTGCTTTTGGATGTGGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCTCTTCTGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAGCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGGCCTCTGGCAGTTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGTTGGTCGATCACTCGatgatgt                          <  1:33599/251‑2 (MQ=255)                                                                                                                                                                                                     ggTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATgg                          >  1:3608/1‑251 (MQ=255)                                                                                                                                                                                                                  ggCGGTGCCGACTCCCGTTTGCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTGTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGcgccg             <  2:179219/251‑1 (MQ=255)                                                                                                                                                                                                                  ggCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGcgccg             >  1:300843/1‑251 (MQ=255)                                                                                                                                                                                                                          cGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGc       >  1:218609/1‑249 (MQ=255)                                                                                                                                                                                                                             cTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGACTGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGCAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGCATCCGCGCCGCGTGGCAGTgg  >  1:169965/1‑251 (MQ=255)                                                                                                                                                                                                                             cTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGATGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTAC‑GTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGAGCCGCGTGGCAgggg  >  1:37820/1‑248 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             AATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCA‑GTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGG  >  NC_000913/4626436‑4626905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑