| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,626,565 | G→T | V218V (GTG→GTT) | radA → | DNA recombination protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,626,565 | 0 | G | T | 90.9% | 67.8 / 0.8 | 33 | V218V (GTG→GTT) | radA | DNA recombination protein |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (15/15); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01 | |||||||||||
GCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAG > NC_000913/4626332‑4626809 | gcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCaa > 1:198132/1‑248 (MQ=255) gCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCtggcgggc < 1:311356/250‑1 (MQ=255) gCCGACAGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGTACACGTAACCAAAGATGGCTCGCTGGCGGGcc < 1:324760/251‑1 (MQ=255) cAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGc > 1:115983/1‑251 (MQ=255) ctcAATATGTTATCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGTAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCCGGTGATGCATATGGCGGATGTTCAGGCATCGCCTGGCAGCGTGGCGCAGGGGCGGGAAACGGCGGCTTATCTGACGCGACTGGCCACAACGCGCGTTGTGGTGATTGT‑CAGGGTTGGACACGTAACCACATAAGGCTCgctggcggggccaaacgttgttgg > 2:318914/1‑233 (MQ=255) cAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTCAAGTGCTGGAac > 1:275427/1‑251 (MQ=255) cAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGGAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGAGTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTg < 2:166659/247‑1 (MQ=255) gtCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCg > 1:263840/1‑251 (MQ=255) cGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGGACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGGGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGGGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATTGGTCGCTGGCGGGCCCTAAAATGC‑GGGACACCTGTATCGAc > 1:317503/1‑251 (MQ=255) aaCCAGCAGCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGGCTCGGTCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCGGGCCCTGAAGTGCTGGAACAC‑TGTATCGACTGtt < 1:189813/251‑1 (MQ=255) aCCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTc < 1:135832/251‑1 (MQ=255) cATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGAGGCGGTTGT‑AATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAAGGCTGGAACAC‑TTTATCAACTGTTCGGTgg > 1:334434/1‑250 (MQ=255) gCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACGCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACTCGCTTCGCCAAAACGCGCGTTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGTCGATGCCTACTc < 1:240628/251‑1 (MQ=255) ccgcagaagaGCAACCGAAG‑CTGATGGAAATTGACTCGAACCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGGGCTTTTGGAGGGCGATGccaacacc > 1:338233/5‑245 (MQ=255) gaagaaGAGCAACCGAAGACTG‑TGGGAATTGCATCGTGCCAGGAGATGCATATGGCGGATGTACGATCCGCGCCTGGCAGCGTTGCGCAGGTTCGTGAAACGTCGGCTTATCTGACGCGCTTCGCCAAAACGCGTGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCACAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGTAACAC‑TGTATCGACTGGTCGGTGCTTTTGGATGGCGATGCCGACTCCCg < 2:306084/251‑1 (MQ=255) cGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGGTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCActtta < 1:324197/251‑5 (MQ=255) aaG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATTCCGACTCCCGTTTTCGCActttacg > 1:361604/1‑245 (MQ=255) gATGGAAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGCTTGT‑CATGGTTGGACCCGTAACCCAAGATGGCTCGCTGGCGGGCCCTAAAGAGCTGGCACAC‑TGTATCGAATGTTCGGCGCTTTTGGGTGGCGATGCCGCCTCCCGTTTTCGCActtttcgccgcc > 1:84402/1‑240 (MQ=255) cagtgtctctaTCCAGGTTAGGCATATGGCGGGTGTGCAGTCATGGCCTGGCAGCGGGGCGCAGGTGCGTGAAACGGTTGCGTATCTGACGCGCTTCGCCAAAACGCGCTGTGTGGCGAA‑GTGCATGGTTGGACACGTACCCAAGGATGGCTCGCTGGCTGGCTCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGAGGGTGATTCCGACTCCCGTTTTCGCACTTTACGCTGTCATaaa < 2:235600/240‑1 (MQ=255) gcaccaggtagtGCATATGTCGGATGTCCAGTCTTCGCCGGGCAGCGGGGCGCAGGTTTGGGAACCGGCGGCTTATCTGACGCGCTGCGCCAAAACGCGCGTTGTGGCGATTGT‑CATGGTTGTACACGTATCCAAAGATGGCTAGCTGGCGGTCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCGTTTGGATGTCGATGCCGACTCCCGTTTGCTCACTTTACGCAGCCATAAAAACCGTTTCg < 2:377895/240‑1 (MQ=255) tGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGGCTGTTCGGTGCTTTTGGGTGGCGATGCCCACTCCCGTTTTCGCACTTTACGCAACCATAAAAACCGCTTCGGCGCGGTg > 1:174780/1‑251 (MQ=255) gCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTggg < 1:226019/251‑1 (MQ=255) ggATGTTCTGTCACCGCCTGGCTGCTTGGGGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCg < 2:361604/251‑1 (MQ=255) aCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCACGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcg < 1:315239/251‑1 (MQ=255) ggcaGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACa < 1:165117/248‑1 (MQ=255) ggcggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGAGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCtt > 1:301318/1‑251 (MQ=255) ctGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTACCCAAAGATGGATCGCTGGCGGGCCCTAAAGTTCTGGAACAC‑TGAATCGACTGTTCGGTGCTTTTGGGTGGCGATACCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCAATTCGGCGCGGTGAAAGAGCCAGGCGTCTTAGCGATGACCGAACAGGGGGTGGGGGAAATCCGCACCCCTTCAGCAAtttt > 2:362152/2‑251 (MQ=255) gacgcGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCt > 1:53149/5‑251 (MQ=255) tCGCCAAAACTCGCGTTGTTGCGATTGT‑CATGGTTGGATACGTAACCAAATATGGCTTGCTTGGGGGCCCTAAATTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGTCTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCTGCGCGGTGAATGAGCTGGTCGGCTTCGCGATGACCGAACAGGGGCTGCGTTAAGTCAGTAACCCTTCGGCAATTTTCTTAAGTcg < 2:334434/250‑1 (MQ=255) cgcgcgGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGCCTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGa > 1:214383/1‑250 (MQ=255) gtgtGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGCCTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGAAGATGAAGTGAcc > 1:47472/1‑251 (MQ=255) ggCGATTGT‑CATGGTTGGACATGTAACCAAAGATGGCACGCTGGTGGGCCCGAAAGTGCAGGAACAC‑TTGATCGACTGTGCGGTTCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACGTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGAGTCTTCGCGATGACCGAACAGGGGATGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGTAGTTACCtac < 2:171989/250‑3 (MQ=255) cATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAATGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGACGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCTCGGCGATGAAGTGACCTCCGGTAGCTCAg < 1:113502/251‑1 (MQ=255) | GCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAG > NC_000913/4626332‑4626809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |