| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,626,521 | T→C | L204L (TTG→CTG) | radA → | DNA recombination protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,626,521 | 0 | T | C | 87.1% | 68.2 / ‑0.3 | 33 | L204L (TTG→CTG) | radA | DNA recombination protein |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (11/16); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
CAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTT > NC_000913/4626275‑4626761 | cagATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGac < 1:117966/251‑1 (MQ=255) aaacgcgttATGTCGCCGGCGAAGAGTCGCTTCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATAGGTTGTCGTAAACCAGCATCGAGCAGATCAGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGTGGC‑TTATCTGACGCGCTTc < 2:220261/243‑1 (MQ=255) gTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATACGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGACTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATGGCCTGGCAGCGTGGAGCAGGTGCGTGAAACGGCGGC‑TTATATGACGCGATTCGCCAAAAagcg > 1:186084/1‑251 (MQ=255) gggtttcttgtacatggggtgaTGCGCGCTCATGGCCTGTGCATGCCGACTTATAATCTCAAGAGGTGGTAGGAAACCATCATCTAACTGATCGTCCTGATGTCCGAAGAAGAGCAACCGATG‑GTGATGGTAATTGACGCGATCCAGGTGATGCAGAGTGCGGATGTACATGCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGTGGC‑TTATTTTGCACTCGTCGTCAATCCGCGCGTTGTGGCGaa < 2:197336/230‑2 (MQ=255) gCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CAt < 1:61018/249‑1 (MQ=255) acacatgTGGCAATGCGCGCACATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTAGGTGACGCGCATCGCCAAAACGCGCGGTTTGGCGATTGT‑CAtggt < 2:61531/245‑1 (MQ=255) tGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATATCACTATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGCTGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTAAAGTCATCGCCTGGCAGCGCGGCGACGGTGCGTGAAACGGAGGCGTTACT‑GACGCGCTTCGCCAAAAAGCGCGGTGTGGCgtctgtcctagttgtgtac > 2:164634/1‑233 (MQ=255) gcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCaa > 1:198132/1‑248 (MQ=255) gCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCtggcgggc < 1:311356/250‑1 (MQ=255) gCCGACAGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGTACACGTAACCAAAGATGGCTCGCTGGCGGGcc < 1:324760/251‑1 (MQ=255) cAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGc > 1:115983/1‑251 (MQ=255) ctcAATATGTTATCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGTAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCCGGTGATGCATATGGCGGATGTTCAGGCATCGCCTGGCAGCGTGGCGCAGGGGCGGGAAACGGCGGC‑TTATCTGACGCGACTGGCCACAACGCGCGTTGTGGTGATTGT‑CAGGGTTGGACACGTAACCACATAAGGCTCgctggcggggccaaacgttgttgg > 2:318914/1‑233 (MQ=255) cAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTCAAGTGCTGGAac > 1:275427/1‑251 (MQ=255) cAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGGAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGAGTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTg < 2:166659/247‑1 (MQ=255) gtCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCg > 1:263840/1‑251 (MQ=255) cGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGGACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGGGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGGGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATTGGTCGCTGGCGGGCCCTAAAATGC‑GGGACACCTGTATCGAc > 1:317503/1‑251 (MQ=255) aaCCAGCAGCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGGCTCGGTCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCGGGCCCTGAAGTGCTGGAACAC‑TGTATCGACTGtt < 1:189813/251‑1 (MQ=255) aCCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTc < 1:135832/251‑1 (MQ=255) cATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGAGGCGGTTGT‑AATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAAGGCTGGAACAC‑TTTATCAACTGTTCGGTgg > 1:334434/1‑250 (MQ=255) gCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACGCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACTCGCTTCGCCAAAACGCGCGTTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGTCGATGCCTACTc < 1:240628/251‑1 (MQ=255) ccgcagaagaGCAACCGAAG‑CTGATGGAAATTGACTCGAACCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGGGCTTTTGGAGGGCGATGccaacacc > 1:338233/5‑245 (MQ=255) gaagaaGAGCAACCGAAGACTG‑TGGGAATTGCATCGTGCCAGGAGATGCATATGGCGGATGTACGATCCGCGCCTGGCAGCGTTGCGCAGGTTCGTGAAACGTCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGTGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCACAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGTAACAC‑TGTATCGACTGGTCGGTGCTTTTGGATGGCGATGCCGACTCCCg < 2:306084/251‑1 (MQ=255) cGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGGTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCActtta < 1:324197/251‑5 (MQ=255) aaG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATTCCGACTCCCGTTTTCGCActttacg > 1:361604/1‑245 (MQ=255) gATGGAAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGCTTGT‑CATGGTTGGACCCGTAACCCAAGATGGCTCGCTGGCGGGCCCTAAAGAGCTGGCACAC‑TGTATCGAATGTTCGGCGCTTTTGGGTGGCGATGCCGCCTCCCGTTTTCGCActtttcgccgcc > 1:84402/1‑240 (MQ=255) cagtgtctctaTCCAGGTTAGGCATATGGCGGGTGTGCAGTCATGGCCTGGCAGCGGGGCGCAGGTGCGTGAAACGGTTGC‑GTATCTGACGCGCTTCGCCAAAACGCGCTGTGTGGCGAA‑GTGCATGGTTGGACACGTACCCAAGGATGGCTCGCTGGCTGGCTCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGAGGGTGATTCCGACTCCCGTTTTCGCACTTTACGCTGTCATaaa < 2:235600/240‑1 (MQ=255) gcaccaggtagtGCATATGTCGGATGTCCAGTCTTCGCCGGGCAGCGGGGCGCAGGTTTGGGAACCGGCGGC‑TTATCTGACGCGCTGCGCCAAAACGCGCGTTGTGGCGATTGT‑CATGGTTGTACACGTATCCAAAGATGGCTAGCTGGCGGTCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCGTTTGGATGTCGATGCCGACTCCCGTTTGCTCACTTTACGCAGCCATAAAAACCGTTTCg < 2:377895/240‑1 (MQ=255) tGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGGCTGTTCGGTGCTTTTGGGTGGCGATGCCCACTCCCGTTTTCGCACTTTACGCAACCATAAAAACCGCTTCGGCGCGGTg > 1:174780/1‑251 (MQ=255) gCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTggg < 1:226019/251‑1 (MQ=255) ggATGTTCTGTCACCGCCTGGCTGCTTGGGGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCg < 2:361604/251‑1 (MQ=255) aCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCACGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcg < 1:315239/251‑1 (MQ=255) ggcaGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACa < 1:165117/248‑1 (MQ=255) ggcggc‑TTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGAGATTGT‑CATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCtt > 1:301318/1‑251 (MQ=255) | CAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAG‑CTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGC‑TTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGT‑CATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTT > NC_000913/4626275‑4626761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |