| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,626,247 | A→G | L112M (CTA→TTG) | radA → | DNA recombination protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,626,247 | 0 | A | G | 94.4% | 100.5 / ‑2.3 | 36 | L112M (CTA→TTG) | radA | DNA recombination protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (15/19); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01 | |||||||||||
TCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGT > NC_000913/4626012‑4626476 | tCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGGGCGGGGGAATCCACGCTGTTGCTGCAAACGCTGTGc > 2:66711/1‑251 (MQ=255) gAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACt > 1:61531/1‑251 (MQ=255) cTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCcagc > 1:254462/1‑250 (MQ=255) cGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGCCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTGTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTc < 1:131355/251‑1 (MQ=255) aGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCa < 1:309908/251‑1 (MQ=255) cGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAAc > 1:159612/1‑251 (MQ=255) aTGCCGGTAGCGCCGGGTTGGCAAAAGTCCAGTAAGTCGCCTATATCAGCCTGGAAGAGCTGCGTCGTTTTGCCCCCGGATTTAAAGCGTTCGACCGCGTACTAGGCGGCGGCGTTGTGCCAGGAAGTGCCATTCGGAATGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGGGCAAACTGGCCGCGCAGATGAAAGCGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTg < 2:326434/251‑1 (MQ=255) ggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATc < 1:99292/251‑1 (MQ=255) aaaGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGACGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTgg > 1:206053/1‑251 (MQ=255) atCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACACTCTCa > 1:384384/1‑250 (MQ=255) tCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGCACCATTATGATTGGCGGTCACACTGGTGCGGGGAAATCCACGATGATGCTGCCAACGCTGTGCCAACTGGCCCAGAAGATGAAAAAGCAGTATGTCACCGGCGAAGCGTCGCTGCAACAGGTGGCAAGGCGCGCTCATAGCCTTGGGCTGGCGCATGACACTCTCAat > 2:365951/1‑251 (MQ=255) aGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAatat > 1:377895/1‑251 (MQ=255) tGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAAcc > 1:179219/1‑251 (MQ=255) gCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGAGTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAAcc < 1:365951/250‑1 (MQ=255) ccGCGTTTTTCCACCTGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAg < 1:233273/251‑1 (MQ=255) cgcgTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGc > 1:235600/1‑251 (MQ=255) tttacACCGGATTTAAAGATGTCGACCGCGTACTTGGCGGCGGCGTGGTGCCAGGAATTGCAATTCGGATTGGCTGTTACCCTGTGGCGGGGAAATCCACGCTGTTGCTGGAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGGATGTCATCGGCGAAGAGTCGCTGCAATAGGTGGCAATGCGCGTGCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGa < 2:225321/246‑1 (MQ=255) cctccGGTTTTAAAGCGTTCGGACGCGTTCTAGGTGGAGGGGTGGTGCCATGAAGTGCCATTCTGATTTGCGGGAACCCTGGGGTGGGGAAATCAACGCTGTTGCTTCAAACGCTGTGCAAACTGGCCTAGCAGATGAAAACGCTGTATGTTACCGTCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGACTTTGCCTGCCGACTGACAATCTCAATAtgttttctgtaatcagcagtgagcag < 2:228873/248‑23 (MQ=255) ccGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATc < 1:10587/251‑1 (MQ=255) cGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGCAACCGCTGTATGTCACCGGCGGAGAGTCGCTGCAACAGGGGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAgctat > 2:135832/1‑247 (MQ=255) agTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGcc < 1:314593/251‑1 (MQ=255) cGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCga > 1:148797/1‑249 (MQ=255) aGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAAcc < 1:51014/249‑1 (MQ=255) ggcggcggcGTGTTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAATCGCTTTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCTGCGAAGAGTCg < 2:342739/135‑1 (MQ=255) ggcggcggcGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGaa < 2:148797/251‑1 (MQ=255) ggcggcggcGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGAAAACGCTGTGCAAACTGGCACAGCAGATGAAAACGCTGTATGTCACAGGCGAAGAGTCg > 1:342739/1‑135 (MQ=255) ggcggcgTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGCTGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAg > 1:54684/1‑206 (MQ=255) ggcggcgTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCt > 1:166659/1‑251 (MQ=255) gggtgCCTGGAAGTGCCCTTCTGATTTGCGGTAACCCTGGGGCGGGGAAATCCACTCTGGTGCTTCTAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGTGAAGAGTCGCTTCAACGGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGGTGTCGGAAACCAGCAGCGAGCATATCTGCCTGATTGTCGAAGATGAGCAACAGAAGCTGATGGTa < 2:254462/250‑1 (MQ=255) gCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATAGCCTTGGCCTGCCGCCTGACAATCTCAATATGATGTCGGAAACCATCATCGAGCAGATCTGCCTGATTGCCGAAGAAAAGCAACCGAAGCTGCTGGTACTTg > 1:356177/1‑251 (MQ=255) ggAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGGCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTc < 1:286953/251‑1 (MQ=255) gggccattgggatgggaggTAACCGAGGTTCGGGGAAATCCACTCTGCTGCTGCAAACGCTGTGCAAACTGGCCGAGCAGATGAAAACTCTGTATGTCACCTGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGAta < 2:206053/234‑2 (MQ=255) aTTCTGATTGGCGGTAACCCTGGTGCGGGGAAAGCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCAGCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGt < 2:159612/251‑1 (MQ=255) tCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTG‑CCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGa < 1:78805/250‑1 (MQ=255) aaCCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCgg < 1:66711/250‑1 (MQ=255) ggCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGt < 1:6628/250‑1 (MQ=255) | TCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGT > NC_000913/4626012‑4626476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |