Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	966	T→C	S210S (TCT→TCC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	966	0	T	C	96.0%	66.6 / ‑5.4	25	S210S (TCT→TCC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (16/8);  total (17/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGG  >  NC_000913/725‑1211                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       cGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACACCGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGAAATGAAAAAGGCGAACTGGTGGTACTTGGGCGCAACGGTTCCGACTCCTCCGCGGCGGTg                                                                                                                                                                                                                                               >  2:150776/1‑251 (MQ=255)      gCCATTATGGCCGGCGTATTAGAAGCGCGCGGGCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTACGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTACTCCGCGGCGGAgctggg                                                                                                                                                                                                                                          >  1:318659/1‑250 (MQ=255)       ccATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAACAACTGCTGGCAGAGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGATGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTgctggct                                                                                                                                                                                                                                         >  1:44685/1‑251 (MQ=255)               gCCGGCGTATTAGAAGAGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTggtt                                                                                                                                                                                                                                 >  1:329575/1‑248 (MQ=255)                       aTTAGAAGCGAGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAACATGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGTGTCCACCCGCCGTATTGAGGAAAGTCGCATTCCGGCTGATCCCATGGTGCTGATGGCAGGTTTAACCGCCGGTAATGAAAAAGGCGTACTGGTGGTCCTGGGACGCAACGGTTCAGACTACGCCGCGGCGGTGAGGGCTACCTGTTTACGCGCCg                                                                                                                                                                                                                         >  2:226888/1‑251 (MQ=255)                                            cGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCAGACTACTCCGCGGCGGTGCTGGCTGCCTGATTGAGCGGCGATTTTTGCGAGATTTggacgg                                                                                                                                                                                                    >  2:272852/1‑251 (MQ=255)                                                                            tgGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGATTCCACCAGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGATTCACCGCCGGTAATGAAACAGGCGAACTGGTGGTACTTGGAAGAAACGGTTCCGACTAATCCGCGGCGGTGCTCGCTGGCTGGTTACTCGACGATTGTTACGGGATGTGGACGTACGTTGAGGGGGGCTTAACCTGCGAccccgtg                                                                                                                                                                    >  2:90780/1‑247 (MQ=255)                                                                                        aTTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGAt                                                                                                                                                        >  1:105616/1‑251 (MQ=255)                                                                                                    cTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTTTTGCGGGATTTGGGCGGACGTTGACGGGGTCTATACCTGCGACCCACGGCCGGTGCCCGATGCGAGGttgttg                                                                                                                                            >  1:338988/1‑251 (MQ=255)                                                                                                            gATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCCCCGATTGTTGCGCGATTTTGCCGCACGGTGCCGGGGTCTATACATGAGCCCCCCGTCAGGGGCCCGATTCGAAGTTGATGGAGTCggt                                                                                                                                    >  1:247810/1‑249 (MQ=255)                                                                                                                                    aTTGCGGCAAGTCGCATCCCGGCTGATCACAGGGTGCTGATGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGc                                                                                                             <  1:93164/250‑1 (MQ=255)                                                                                                                                          gCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTACTGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGCTGGAGCTTTCCt                                                                                                       >  1:129529/1‑250 (MQ=255)                                                                                                                                              gtcGCATTCCGGTTGATCACATGGTGCGGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTc                                                                                                  <  1:208118/249‑1 (MQ=255)                                                                                                                                                           tGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACCCGCCGATTGTTGCGAGGTTTGGAGGGGCGTTGGCGGGGTCTATACCTTCGACCCGCGGCAGGGGCCCGATGCGGGGGTTTTGAAGCCGATTCCCTACCAGGAAGGGATGGAGCTTTCCCATTTCAGCGTT‑AAAGttt                                                                                    >  1:178431/1‑250 (MQ=255)                                                                                                                                                               cacaTGGTGCTGAGGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCa                                                                                <  1:54952/251‑1 (MQ=255)                                                                                                                                                                         tGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGGTTGTTGCGGGATTTGGACGGACGTTGACGGGGGCTTTTCCTGCGACCCGCGTCAGGTGCCCGATGCggggg                                                                                                                                                 >  1:197309/1‑173 (MQ=255)                                                                                                                                                                               cAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTaac                                                                >  1:10682/1‑249 (MQ=255)                                                                                                                                                                                     tCACCGCCGGTAATGAAAAAGGCGAACTGGTGTTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCAt                                                           <  1:204621/250‑1 (MQ=255)                                                                                                                                                                                        ccgccgGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCCTCGcc                                                       >  1:373718/1‑251 (MQ=255)                                                                                                                                                                                         cgcaggTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTTCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCATCGccc                                                      <  2:96321/247‑1 (MQ=255)                                                                                                                                                                                          gccgGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCa                                                     <  1:166970/251‑1 (MQ=255)                                                                                                                                                                                                                 ggtggtACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGAt                                <  1:44618/249‑1 (MQ=255)                                                                                                                                                                                                                            gACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGa                   <  1:245119/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTTACGGGGTCTATACCTGCGAACCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGACCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTTAAAGT‑CTTCACCCCCGCACCATTACCCCCCTAGCCCACTTCCAGAACCCCTGCCTGATTTAAAATACCGGaaaacattc           >  1:374959/1‑245 (MQ=255)                                                                                                                                                                                                                                             tactCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAGTACCGGAAATCCTCAAGCACCAgg  >  1:110783/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       CGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCT‑AAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGG  >  NC_000913/725‑1211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑