Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,245	C→T	L112M (CTA→TTG)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,245	0	C	T	91.2%	86.5 / ‑1.3	36	L112M (CTA→TTG)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (14/17);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGT  >  NC_000913/4626012‑4626476                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        tCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGGGCGGGGGAATCCACGCTGTTGCTGCAAACGCTGTGc                                                                                                                                                                                                                        >  2:66711/1‑251 (MQ=255)      gAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACt                                                                                                                                                                                                                   >  1:61531/1‑251 (MQ=255)               cTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCcagc                                                                                                                                                                                                           >  1:254462/1‑250 (MQ=255)                                                cGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGCCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTGTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTc                                                                                                                                                                         <  1:131355/251‑1 (MQ=255)                                                      aGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCa                                                                                                                                                                   <  1:309908/251‑1 (MQ=255)                                                        cGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAAc                                                                                                                                                                 >  1:159612/1‑251 (MQ=255)                                                             aTGCCGGTAGCGCCGGGTTGGCAAAAGTCCAGTAAGTCGCCTATATCAGCCTGGAAGAGCTGCGTCGTTTTGCCCCCGGATTTAAAGCGTTCGACCGCGTACTAGGCGGCGGCGTTGTGCCAGGAAGTGCCATTCGGAATGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGGGCAAACTGGCCGCGCAGATGAAAGCGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTg                                                                                                                                                            <  2:326434/251‑1 (MQ=255)                                                                             ggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATc                                                                                                                                            <  1:99292/251‑1 (MQ=255)                                                                                    aaaGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGACGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTgg                                                                                                                                     >  1:206053/1‑251 (MQ=255)                                                                                                         atCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACACTCTCa                                                                                                                 >  1:384384/1‑250 (MQ=255)                                                                                                          tCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGCACCATTATGATTGGCGGTCACACTGGTGCGGGGAAATCCACGATGATGCTGCCAACGCTGTGCCAACTGGCCCAGAAGATGAAAAAGCAGTATGTCACCGGCGAAGCGTCGCTGCAACAGGTGGCAAGGCGCGCTCATAGCCTTGGGCTGGCGCATGACACTCTCAat                                                                                                               >  2:365951/1‑251 (MQ=255)                                                                                                            aGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAatat                                                                                                             >  1:377895/1‑251 (MQ=255)                                                                                                                         tGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAAcc                                                                                                >  1:179219/1‑251 (MQ=255)                                                                                                                          gCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGAGTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAAcc                                                                                                <  1:365951/250‑1 (MQ=255)                                                                                                                           ccGCGTTTTTCCACCTGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAg                                                                                              <  1:233273/251‑1 (MQ=255)                                                                                                                            cgcgTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGc                                                                                             >  1:235600/1‑251 (MQ=255)                                                                                                                                  tttacACCGGATTTAAAGATGTCGACCGCGTACTTGGCGGCGGCGTGGTGCCAGGAATTGCAATTCGGATTGGCTGTTACCCTGTGGCGGGGAAATCCACGCTGTTGCTGGAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGGATGTCATCGGCGAAGAGTCGCTGCAATAGGTGGCAATGCGCGTGCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGa                                                                                        <  2:225321/246‑1 (MQ=255)                                                                                                                                     cctccGGTTTTAAAGCGTTCGGACGCGTTCTAGGTGGAGGGGTGGTGCCATGAAGTGCCATTCTGATTTGCGGGAACCCTGGGGTGGGGAAATCAACGCTGTTGCTTCAAACGCTGTGCAAACTGGCCTAGCAGATGAAAACGCTGTATGTTACCGTCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGACTTTGCCTGCCGACTGACAATCTCAATAtgttttctgtaatcagcagtgagcag                                                                                    <  2:228873/248‑23 (MQ=255)                                                                                                                                        ccGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATc                                                                                 <  1:10587/251‑1 (MQ=255)                                                                                                                                         cGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGCAACCGCTGTATGTCACCGGCGGAGAGTCGCTGCAACAGGGGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAgctat                                                                                >  2:135832/1‑247 (MQ=255)                                                                                                                                                    agTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGcc                                                                     <  1:314593/251‑1 (MQ=255)                                                                                                                                                        cGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCga                                                                   >  1:148797/1‑249 (MQ=255)                                                                                                                                                                    aGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAAcc                                                       <  1:51014/249‑1 (MQ=255)                                                                                                                                                                     ggcggcggcGTGTTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAATCGCTTTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCTGCGAAGAGTCg                                                                                                                                                                        <  2:342739/135‑1 (MQ=255)                                                                                                                                                                     ggcggcggcGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGaa                                                    <  2:148797/251‑1 (MQ=255)                                                                                                                                                                     ggcggcggcGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGAAAACGCTGTGCAAACTGGCACAGCAGATGAAAACGCTGTATGTCACAGGCGAAGAGTCg                                                                                                                                                                        >  1:342739/1‑135 (MQ=255)                                                                                                                                                                        ggcggcgTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGCTGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAg                                                                                              >  1:54684/1‑206 (MQ=255)                                                                                                                                                                        ggcggcgTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCt                                                 >  1:166659/1‑251 (MQ=255)                                                                                                                                                                               gggtgCCTGGAAGTGCCCTTCTGATTTGCGGTAACCCTGGGGCGGGGAAATCCACTCTGGTGCTTCTAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGTGAAGAGTCGCTTCAACGGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGGTGTCGGAAACCAGCAGCGAGCATATCTGCCTGATTGTCGAAGATGAGCAACAGAAGCTGATGGTa                                          <  2:254462/250‑1 (MQ=255)                                                                                                                                                                                   gCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATAGCCTTGGCCTGCCGCCTGACAATCTCAATATGATGTCGGAAACCATCATCGAGCAGATCTGCCTGATTGCCGAAGAAAAGCAACCGAAGCTGCTGGTACTTg                                      >  1:356177/1‑251 (MQ=255)                                                                                                                                                                                       ggAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGGCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTc                                  <  1:286953/251‑1 (MQ=255)                                                                                                                                                                                           gggccattgggatgggaggTAACCGAGGTTCGGGGAAATCCACTCTGCTGCTGCAAACGCTGTGCAAACTGGCCGAGCAGATGAAAACTCTGTATGTCACCTGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGAta                              <  2:206053/234‑2 (MQ=255)                                                                                                                                                                                                aTTCTGATTGGCGGTAACCCTGGTGCGGGGAAAGCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCAGCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGt                         <  2:159612/251‑1 (MQ=255)                                                                                                                                                                                                  tCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTG‑CCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGa                       <  1:78805/250‑1 (MQ=255)                                                                                                                                                                                                               aaCCCTGGTGCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCgg           <  1:66711/250‑1 (MQ=255)                                                                                                                                                                                                                       ggCGGGGAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGt  <  1:6628/250‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        TCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGT  >  NC_000913/4626012‑4626476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑