| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,625,546 | C→T | L218L (CTG→TTG) | serB → | phosphoserine phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,625,546 | 0 | C | T | 86.4% | 46.0 / ‑1.7 | 22 | L218L (CTG→TTG) | serB | phosphoserine phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (13/6); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATACCTGCGCGACAAGCTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGT > NC_000913/4625301‑4625796 | cGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGAGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGAGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCACATGGTGCTCAAGCTGGAAACGCTGGGCTGGAGCGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGAGCGACAAGTTgcgc > 1:362267/1‑251 (MQ=255) gggaTGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCATGCGCAGCCGCGTGGCGACGCTGAAAGGAGCAGACGCCAATATTCTGCAACAAGTGCGTGAAAATATGACGCTGATACCAGGCGTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAACGCGGAGATTGACTCCGGGGGGCTTACTTTCTTTGCTGTATATCTGCGCGACAAGTTGCGCATGAc > 2:107381/3‑251 (MQ=255) agaTGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGAcc > 1:225988/1‑251 (MQ=255) aacggtttttcctccccAGCCTGCGCTCCCGGGTGGCAACGGTGAAAGGCGCTGGCGCTAATATTCTGTACCAGTTTCGGGAAAACGTGCGGCGGATCCCAGGCAAACCGCAACCGGGGCTCAAGCTGAGAACGCTGGGCGGGAAGTTGGGGATGGCTCCCGGCGTCTGTACTTTTTTTGCTAAATATCTTGGCGTCAAGTGGCGCCTGACCGCCGTGGTAGCCAATGAACTGTAGAGCATGGACGGTaaa < 2:299465/236‑1 (MQ=255) cTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAAtt > 1:383967/1‑251 (MQ=255) tACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGc > 1:268364/1‑250 (MQ=255) cGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCAAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAAtg < 1:12867/251‑1 (MQ=255) gcgcAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCgg < 1:64393/249‑1 (MQ=255) aGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGAGACAt > 1:369522/1‑251 (MQ=255) tGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGAAATGTGATCGGCGACATCGTAGACGCGCAGTAc > 1:355988/1‑251 (MQ=255) tGAAAGGCGCTGACGCCAATATTCTGCAACAGGAGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAACCGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAAATCATGGACGGTAAATTTACCTGCAATGTGAACGGCGGCATCGTCGACGCGCAgaac > 1:355991/1‑248 (MQ=255) gACGCCAATATACTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAAc < 1:244177/251‑1 (MQ=255) aaCAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGCAAACTCTGACTCGCCTcgcg > 1:129821/1‑251 (MQ=255) aGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCACATGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATAGCCTCAGGCGGCTTTACTTTCTTTGCTGAATCCCTGCGCGACAAGCTGCGCCTGACCGCAGTGGTAGCCAATGAACTGTAGATCATGGACCGTAAATTTACAGGAAATGTGATCGGCGTCATCGTAGACGAGCCGTACAAAGCGACAACTCTGACTCGCCTcgcggtg > 2:364833/1‑248 (MQ=255) tcTGATACCAGACTTAACACAACTGTTGCTCAAGCTGGAAACGCTGGTCTGAAAAGTGGAGATTGCCTCCGTCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACACGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGAAAATTTACCGCCAATGAGCACGGCCTCACCGTCGACGCGCAGTACAACGCGAATACCCTGACTCGCCTCACGCAGGAGTCTGAAATACCGCttg > 2:223090/2‑249 (MQ=255) gCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGtggcgattg < 1:198825/251‑1 (MQ=255) aTTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGcg > 1:110396/1‑250 (MQ=255) cTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTAcc > 1:8670/1‑251 (MQ=255) tcctttctttGCTGAATACCTGCGCGACAAGCTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCAt < 1:161433/248‑1 (MQ=255) ttctttGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGTGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATgcca < 1:287385/250‑1 (MQ=255) ttGCTTAATCCCTGCGCGACAAGCTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATgccaagcc < 1:364833/250‑1 (MQ=255) cTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGtgaatg > 1:167047/1‑250 (MQ=255) cTGCGCCTGACCGCCGTGGTAGCCATTGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGt > 1:125708/1‑251 (MQ=255) | CGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATACCTGCGCGACAAGCTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGT > NC_000913/4625301‑4625796 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |