Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,625,242 T→C D116D (GAT→GAC)  serB → phosphoserine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,625,2420TC89.7% 66.3 / ‑0.0 30D116D (GAT→GAC) serBphosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (13/13);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

ACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGC  >  NC_000913/4624995‑4625485
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   
ccgacggtctttgCGGCTGGCTGCTGTATGGTAGTGGGATGGATAAACATCGGCTGACCCAATACCAGAGCAAACTGGGGGCGGCGAGGGTGATGGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTGCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATg                                                                                                                                                                                                                                                  <  2:142139/239‑1 (MQ=255)
 cGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATgg                                                                                                                                                                                                                                                 >  1:10222/1‑251 (MQ=255)
       ccGTAGCGGCTGGCTGCTGAATGGTCGTGGGCTGGATAAACATCGTCTGACCCAATCCCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCGGGTGCGTGAAAGATTAGCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGACACGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTT‑CTGGTGATGGACATGGATTCCa                                                                                                                                                                                                                                           <  2:76472/250‑1 (MQ=255)
         gTAGCGGCTGTCTGCTGTATGGTCGTGGGCGGGATAAACAGCGGTTGCCCCCATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCAcc                                                                                                                                                                                                                                         <  2:314099/251‑1 (MQ=255)
                                   gggCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGTCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGAGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGCTGGAAATGGACTCCACCGCCATCCAGATTGAATGTATtggtgg                                                                                                                                                                                                               >  2:163712/1‑247 (MQ=255)
                                   gggCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGAGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtgatg                                                                                                                                                                                                                <  1:163712/250‑1 (MQ=255)
                                          aTAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGcc                                                                                                                                                                                                        <  1:284465/251‑1 (MQ=255)
                                                                        aaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTATGGCAGGTTCACTCACCGCCCGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGG‑CAAATCCCGCACCTGCGCACGCCGGGCTTG‑CTGGGGAGGGATATAGACTCCACCGCCATCCAGATTGCATGTATTGCTGAAATTGCCAAACTGGCCGGGACGGGCgagttgtgagcg                                                                                                                                                                          >  2:161433/1‑242 (MQ=255)
                                                                            gtgTGCCGCGATGGTTATTGTTGTCGCCTGTTTCGTGTAAGATTTTCCTGTGATTCGTCTTGCAGGTGCACTATCCGCCCGGGCTTCGCGCCTGGCCCAGGAATAGCCTCTGGTTGTCGCCTCGATGTGG‑AAAATCCCGCACCTGCGCACGCCGGGTGTG‑CTGTTGATGGACATTTATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCATACTGGCCGGAACGGGCGAGAtgttgtcgta                                                                                                                                                                        <  2:324834/247‑9 (MQ=255)
                                                                            gggTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTACCAGGTGATTCGTCTGGCAGGTGCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAg                                                                                                                                                                      <  1:56970/251‑1 (MQ=255)
                                                                                     gATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCAATGAAGCGCAACTGGATGTCGCCCCGCTGGGC‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGCAACCGAAc                                                                                                                                                             >  1:364080/1‑251 (MQ=255)
                                                                                        ggTGTTTGTTGCCGCCTGGGGCGTGGAAGATTATCAGGGTATTCGGCTGGCAGGTTCACTCCCCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCTCCTGCGCACGCCGGGTTTG‑CTGGTGAGGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACggg                                                                                                                                                          <  2:378211/251‑1 (MQ=255)
                                                                                           gATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCg                                                                                                                                                        >  1:118467/1‑250 (MQ=255)
                                                                                                         ggTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTc                                                                                                                                         <  1:273034/251‑1 (MQ=255)
                                                                                                             cGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGAtt                                                                                                                                     >  1:291949/1‑251 (MQ=255)
                                                                                                                          cAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCt                                                                                                                        >  1:46679/1‑251 (MQ=255)
                                                                                                                                                           cGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTg                                                                                        >  1:108742/1‑250 (MQ=255)
                                                                                                                                                                       cTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGAAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGGGGCGACGCTGAAAGGCGCTGACGCCACTATTCt                                                                           >  1:276405/1‑251 (MQ=255)
                                                                                                                                                                              agaaggagcactgggATGCCGCCCGGTTGGGG‑AAACCCCCCCCCCTGCGCACGC‑TGGTTTGTCTGGTGATGGACATGAATTCCCCCGCCATCCAGATTGAATGTATGGATAAAATTGCCAAACTGGCCGGAACGGGCGAGTGGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAg                                                                    <  2:69716/238‑1 (MQ=255)
                                                                                                                                                                                        acTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGaa                                                           >  1:82004/2‑250 (MQ=255)
                                                                                                                                                                                           gtaTGTCGCCCCG‑TGGGGTAAAATCCCGCTCCGGCGCCCGCCGGGTTTG‑CTGGTGATGGGCATGGATTCCCCCGCCATCCAGAGTGATTGTATTGATGAAATTGCCAAACGGGCCGGAACGGGCGAGAGGGTGGCGGAAGTAACCGAACGGCCGATGCGCGGTGACCTCGATTTTCCCTCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGGGAAAATc                                                       <  1:242192/249‑1 (MQ=255)
                                                                                                                                                                                             aTGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTg                                                     >  1:281338/1‑251 (MQ=255)
                                                                                                                                                                                                          ggaggatttcccctactcTGCGCACGTCTGGTTTG‑CTGGTGAGTGACATGATATCCGTCGCCATCCAGATTGAATCTTTGGATAAAATTGCCAAATGGGCCGGACCGGGCGTGTTGGTGGCGGAAGTACCCGAACTGGCGTTGCGCTGCGAACTCGATTTTACCGCCATCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGGGCGTGAAAATCTGCCGCTGATGCCa                                         <  2:10222/234‑1 (MQ=255)
                                                                                                                                                                                                           ggg‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCAAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGGGGCGACGCTGAAATGCGCTGACGCCAATATGCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGGCAgg                                       >  2:287385/1‑251 (MQ=255)
                                                                                                                                                                                                                     ccGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGc                                                                                                                                                         >  1:156430/1‑128 (MQ=255)
                                                                                                                                                                                                                     ccGCACCTGCGCACGCCGGGTGTG‑CTGGGGATGGACATGGATGCCACCGCCAGCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGATATGGTGGCGGAAGTAACCGAACGGGc                                                                                                                                                         <  2:156430/128‑1 (MQ=255)
                                                                                                                                                                                                                      cGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCa                              >  1:110475/1‑250 (MQ=255)
                                                                                                                                                                                                                          ccTGCGCACGCCGGGTTTG‑CTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTgg                         <  1:331062/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 gTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctggg   >  1:263125/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  tGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAACATCTGCCGCTGCTGCCCGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgggc  >  2:198825/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   
ACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGG‑AAAATCCCGCACCTGCGCACGCCGGGTTTG‑CTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGC  >  NC_000913/4624995‑4625485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑