Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,517	A→G	G91G (GGT→GGC)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,517	0	A	G	91.9%	95.2 / ‑1.4	38	G91G (GGT→GGC)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (20/14);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

GTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGA  >  NC_000913/4624273‑4624757                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCcgcg                                                                                                                                                                                                                                             >  1:141239/1‑251 (MQ=255)   gaaagcagcAACATCAGGCGTAAAATGTTAGTGGTGTTATCCCCCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTTGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTc                                                                                                                                                                                                                                           <  1:13240/248‑1 (MQ=255)         agcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGAt                                                                                                                                                                                                                                     <  1:26266/251‑1 (MQ=255)          gcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGAt                                                                                                                                                                                                                                     >  1:222245/1‑250 (MQ=255)                   ggCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTc                                                                                                                                                                                                                            >  1:336467/1‑250 (MQ=255)                            tGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc                                                                                                                                                                                                                  >  1:324571/1‑251 (MQ=255)                             gtgtgtgggtgtctcctccttttGGGCGTCGGTGGCGGGCGTATGGGTGTCGAGCGGTAGGCGCAGATAGCCGAGCGGTC‑GTGTTTTCCCGGCAATGGGTGCGACAATCTGCTGGTGAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACGTCGACGCTTTCGCCAGAACGGGCGATAAGATCTCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc                                                                                                                                                                                                                  <  2:136968/229‑1 (MQ=255)                                        tATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGAGTAAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGGTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCCATTCGACGCTTTCGCCAGAACGGGCGATAAGGTCGACTTGTTCGTCATACACGCCCGCCGCGAGGTTACGGATTTCATCCGTTTACTGCACGAGGagc                                                                                                                                                                                                      >  2:106966/1‑249 (MQ=255)                                             cacgtttGGGCTTCGGGTGCGGGCGTATGGGTGGCGAGCGGCAGGTGCCGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCTCTTTTTTACCGTCGGGCGCCTGCCGGTCGCGCACTTCGACTCTTTCGCCATAACGGGCGATAAGATCGCCTTGGTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTg                                                                                                                                                                                                 <  2:193796/246‑1 (MQ=255)                                              ccTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGa                                                                                                                                                                                                <  1:246161/251‑1 (MQ=255)                                                    gggCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATCAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGt                                                                                                                                                                                          >  1:158659/1‑251 (MQ=255)                                                        ttCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGttttt                                                                                                                                                                                      >  1:307536/1‑251 (MQ=255)                                                                       aTGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCgg                                                                                                                                                                       >  1:271081/1‑251 (MQ=255)                                                                                    gtCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCgg                                                                                                                                                          >  1:150564/1‑251 (MQ=255)                                                                                                    cGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGCTACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGACTGCGTTTTTCATCCGGCGAGCCGGTACGCATCAGCGGTGCAACGTTCAGCGtt                                                                                                                                          >  1:247334/1‑250 (MQ=255)                                                                                                          gTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTgg                                                                                                                                    >  1:259552/1‑250 (MQ=255)                                                                                                          gTCCGT‑TTTTCCCGGCAATTGGTTCGACAACCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATCCACGCCCGCGTCGAGGATACGGCTTTCATCCGTTATCTGATCGAGGATCGCCTGAATGCGTTGTTCATCCGTCGAGTCGGTACGCATCAGCGGTGCAACTTTCAGCGTTACCTGa                                                                                                                                    <  1:331406/251‑1 (MQ=255)                                                                                                                     cccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAgg                                                                                                                           <  1:130340/246‑1 (MQ=255)                                                                                                                            aTTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGc                                                                                                                   >  1:96470/1‑251 (MQ=255)                                                                                                                                  tCGACAATCTGCGGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGtt                                                                                                              <  1:58309/250‑1 (MQ=255)                                                                                                                                    gACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCAAGCCGGTCGCGCACTTCGACGCTTTCGCACGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGAAACGGCTTTCATACGTTAACTGCTCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGCTCAGCGTTACCTGACGCGCCAGGGTGAGGGCCAGttctt                                                                                                           >  1:182916/1‑251 (MQ=255)                                                                                                                                                  tAAAATAGCCGCCCGCTTTTTTACCGTCTAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGCGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGGGCAAAGTTCAGCGTTACCTGACGCGCCAGGGGGCGGGCCCGTTCTTCCGGCTGTgggta                                                                                              >  2:284025/1‑247 (MQ=255)                                                                                                                                                   aaaaTAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTGTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTa                                                                                             <  1:284025/250‑1 (MQ=255)                                                                                                                                                       tAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGCTAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACgttg                                                                                        >  1:279220/1‑248 (MQ=255)                                                                                                                                                         gcctcccgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGACGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTc                                                                                      <  1:80294/244‑1 (MQ=255)                                                                                                                                                                   tttttACCGTCGGGCCCCAGCCGGTCGCGCACTTCGACGCTTTCGCCCGAACGTGCGATAAGACCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTggtggt                                                                             <  2:96470/250‑1 (MQ=255)                                                                                                                                                                             cGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAAc                                                                  <  1:295830/251‑1 (MQ=255)                                                                                                                                                                                 cgcCAGCCGGTCGCGAACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTCCCTGACGCGCCAGGGTGCGGGCCAGTTCTTCAAGCTGTGGATTACGTTGTCGCTGGTGGTTTTAACTAAACAAT‑g                                                             >  1:187333/1‑251 (MQ=255)                                                                                                                                                                                             gcgcACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGGTCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGc                                                  >  1:43477/1‑250 (MQ=255)                                                                                                                                                                                                            ttCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACTAAc                                   <  1:64123/250‑1 (MQ=255)                                                                                                                                                                                                               gCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGCGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGAGCCAGGGAGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCAGCAACGCCCCTAACaggg                               >  1:55798/1‑248 (MQ=255)                                                                                                                                                                                                                     acgggCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGAGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACTAACACGGCAAGAc                         >  1:58416/5‑251 (MQ=255)                                                                                                                                                                                                                      cgggCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGCTCGAGGATCGCCTGAATGCGTTTTTCATACGGCGAGTCGGTACGCATCAGCGGGGCAACGTTCAGCGTTACCTGACGCCCCAGGGGGCGGGCCCGTTCATCCAGCTGTGGATGACGTTTTCGCTGGTGGTTTTGCATAACCCATTGAC‑CTCCCAGCATCCGCGCCCCAACCAAGGCCAGAc                         >  2:195673/4‑250 (MQ=255)                                                                                                                                                                                                                      cgggCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACa                        <  1:332829/248‑1 (MQ=255)                                                                                                                                                                                                                      cgggCGATAAGATAGACTTGTTCGTCCTACACGCCCGCGACGAGGATACGGCTTTCATACGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCCGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCAAGCTGTGGATTACGTTGTAGCTGGTGGTTTTGCCTAAACCAT‑GACGCTCCCTGCATAAGCGACACTACTAAGGCAAgcc                         >  2:321267/4‑248 (MQ=255)                                                                                                                                                                                                                            agaaGATCGCTTTTTTCGTCATACACGCCCGCGGCTATGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCACCCGGCGAGTCGGTACGCATCAGCGTTGCAACTTTCAGCGTTACCTGACGCGTCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAg                  <  2:324571/249‑1 (MQ=255)                                                                                                                                                                                                                                           tCGTCATACTCTCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACTTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCGGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACTAACACGGCAATACAGAACAGGACAATCTCTGccc    <  2:307536/250‑1 (MQ=255)                                                                                                                                                                                                                                            cGTCATACACACCCGCGTCGAGGATACGTCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCTTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACGATCACTGCCCgg  >  1:41237/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCAT‑GACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGA  >  NC_000913/4624273‑4624757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑