| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,624,487 | T→G | A101A (GCA→GCC) | ytjB ← | putative inner membrane protein Smp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,624,487 | 0 | T | G | 93.5% | 86.3 / ‑2.6 | 31 | A101A (GCA→GCC) | ytjB | putative inner membrane protein Smp |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (17/12); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
GTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGG > NC_000913/4624273‑4624728 | gTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCcgcg > 1:141239/1‑251 (MQ=255) gaaagcagcAACATCAGGCGTAAAATGTTAGTGGTGTTATCCCCCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTTGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTc < 1:13240/248‑1 (MQ=255) agcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGAt < 1:26266/251‑1 (MQ=255) gcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGAt > 1:222245/1‑250 (MQ=255) ggCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTc > 1:336467/1‑250 (MQ=255) tGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc > 1:324571/1‑251 (MQ=255) gtgtgtgggtgtctcctccttttGGGCGTCGGTGGCGGGCGTATGGGTGTCGAGCGGTAGGCGCAGATAGCCGAGCGGTC‑GTGTTTTCCCGGCAATGGGTGCGACAATCTGCTGGTGAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACGTCGACGCTTTCGCCAGAACGGGCGATAAGATCTCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc < 2:136968/229‑1 (MQ=255) tATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGAGTAAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGGTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCCATTCGACGCTTTCGCCAGAACGGGCGATAAGGTCGACTTGTTCGTCATACACGCCCGCCGCGAGGTTACGGATTTCATCCGTTTACTGCACGAGGagc > 2:106966/1‑249 (MQ=255) cacgtttGGGCTTCGGGTGCGGGCGTATGGGTGGCGAGCGGCAGGTGCCGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCTCTTTTTTACCGTCGGGCGCCTGCCGGTCGCGCACTTCGACTCTTTCGCCATAACGGGCGATAAGATCGCCTTGGTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTg < 2:193796/246‑1 (MQ=255) ccTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGa < 1:246161/251‑1 (MQ=255) gggCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATCAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGt > 1:158659/1‑251 (MQ=255) ttCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGttttt > 1:307536/1‑251 (MQ=255) aTGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCgg > 1:271081/1‑251 (MQ=255) gtCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCgg > 1:150564/1‑251 (MQ=255) cGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGCTACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGACTGCGTTTTTCATCCGGCGAGCCGGTACGCATCAGCGGTGCAACGTTCAGCGtt > 1:247334/1‑250 (MQ=255) gTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTgg > 1:259552/1‑250 (MQ=255) gTCCGT‑TTTTCCCGGCAATTGGTTCGACAACCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATCCACGCCCGCGTCGAGGATACGGCTTTCATCCGTTATCTGATCGAGGATCGCCTGAATGCGTTGTTCATCCGTCGAGTCGGTACGCATCAGCGGTGCAACTTTCAGCGTTACCTGa < 1:331406/251‑1 (MQ=255) cccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAgg < 1:130340/246‑1 (MQ=255) aTTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGc > 1:96470/1‑251 (MQ=255) tCGACAATCTGCGGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGtt < 1:58309/250‑1 (MQ=255) gACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCAAGCCGGTCGCGCACTTCGACGCTTTCGCACGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGAAACGGCTTTCATACGTTAACTGCTCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGCTCAGCGTTACCTGACGCGCCAGGGTGAGGGCCAGttctt > 1:182916/1‑251 (MQ=255) tAAAATAGCCGCCCGCTTTTTTACCGTCTAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGCGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGGGCAAAGTTCAGCGTTACCTGACGCGCCAGGGGGCGGGCCCGTTCTTCCGGCTGTgggta > 2:284025/1‑247 (MQ=255) aaaaTAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTGTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTa < 1:284025/250‑1 (MQ=255) tAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGCTAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACgttg > 1:279220/1‑248 (MQ=255) gcctcccgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGACGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTc < 1:80294/244‑1 (MQ=255) tttttACCGTCGGGCCCCAGCCGGTCGCGCACTTCGACGCTTTCGCCCGAACGTGCGATAAGACCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTggtggt < 2:96470/250‑1 (MQ=255) cGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAAc < 1:295830/251‑1 (MQ=255) cgcCAGCCGGTCGCGAACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTCCCTGACGCGCCAGGGTGCGGGCCAGTTCTTCAAGCTGTGGATTACGTTGTCGCTGGTGGTTTTAACTAAACAATg > 1:187333/1‑251 (MQ=255) gcgcACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGGTCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGc > 1:43477/1‑250 (MQ=255) ttCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAAc < 1:64123/250‑1 (MQ=255) gCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGCGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGAGCCAGGGAGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCAGCAACGCCCCTAACaggg > 1:55798/1‑248 (MQ=255) | GTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGG > NC_000913/4624273‑4624728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |