Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,400	C→T	E130E (GAG→GAA)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,400	0	C	T	91.7%	62.8 / ‑6.1	25	E130E (GAG→GAA)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (11/11);  minor base G (2/0);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGC  >  NC_000913/4624157‑4624645                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccggCTTTCTCCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGAGTGCTGCCAGCGGGTTCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACaa                                                                                                                                                                                                                                                <  2:129525/248‑1 (MQ=255)  tcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTTCCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGGTTGCCAGTGAAAGCAGCAACATCCGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGGGCGTCAGGCGCAGATAGCCGCGCGGGCCGG‑TTTTCCCGGCGATTGGTTCGACAAt                                                                                                                                                                                                                                               >  1:1916/1‑251 (MQ=255)         cttcctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGGGCGGGTCCGCACTACGCCGATTGCCAGTGAAAGCAGCCACATCAGGCGTAAAATGTTAGGGGTGTTATCCACCTGTTGGGGTTCGGTGGCGAGCGTATGGGGGACGAGCGTCAGGCGCAGATAACCGAGCGGTCCGG‑TTTTCCCGGCAAATGGGTCGACAATCTGCTgg                                                                                                                                                                                                                                        >  1:165534/1‑251 (MQ=255)                 ccgggCATCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTTCGTTTACCCGGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTGAAAATa                                                                                                                                                                                                                                <  2:165534/248‑1 (MQ=255)                        ccGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAgccgccc                                                                                                                                                                                                                         <  1:195674/251‑2 (MQ=255)                                                         gctgcCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTcgcg                                                                                                                                                                                         <  1:26192/250‑1 (MQ=255)                                                                                                                     gTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCcgcg                                                                                                                            >  1:141239/1‑251 (MQ=255)                                                                                                                       gaaagcagcAACATCAGGCGTAAAATGTTAGTGGTGTTATCCCCCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTTGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTc                                                                                                                          <  1:13240/248‑1 (MQ=255)                                                                                                                             agcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGAt                                                                                                                    <  1:26266/251‑1 (MQ=255)                                                                                                                              gcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGAt                                                                                                                    >  1:222245/1‑250 (MQ=255)                                                                                                                                       ggCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTc                                                                                                           >  1:336467/1‑250 (MQ=255)                                                                                                                                                tGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc                                                                                                 >  1:324571/1‑251 (MQ=255)                                                                                                                                                 gtgtgtgggtgtctcctccttttGGGCGTCGGTGGCGGGCGTATGGGTGTCGAGCGGTAGGCGCAGATAGCCGAGCGGTC‑GTGTTTTCCCGGCAATGGGTGCGACAATCTGCTGGTGAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACGTCGACGCTTTCGCCAGAACGGGCGATAAGATCTCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc                                                                                                 <  2:136968/229‑1 (MQ=255)                                                                                                                                                            tATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGAGTAAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGGTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCCATTCGACGCTTTCGCCAGAACGGGCGATAAGGTCGACTTGTTCGTCATACACGCCCGCCGCGAGGTTACGGATTTCATCCGTTTACTGCACGAGGagc                                                                                     >  2:106966/1‑249 (MQ=255)                                                                                                                                                                 cacgtttGGGCTTCGGGTGCGGGCGTATGGGTGGCGAGCGGCAGGTGCCGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCTCTTTTTTACCGTCGGGCGCCTGCCGGTCGCGCACTTCGACTCTTTCGCCATAACGGGCGATAAGATCGCCTTGGTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTg                                                                                <  2:193796/246‑1 (MQ=255)                                                                                                                                                                  ccTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGa                                                                               <  1:246161/251‑1 (MQ=255)                                                                                                                                                                        gggCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATCAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGt                                                                         >  1:158659/1‑251 (MQ=255)                                                                                                                                                                            ttCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGttttt                                                                     >  1:307536/1‑251 (MQ=255)                                                                                                                                                                                           aTGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCgg                                                      >  1:271081/1‑251 (MQ=255)                                                                                                                                                                                                        gtCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCgg                                         >  1:150564/1‑251 (MQ=255)                                                                                                                                                                                                                        cGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGCTACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGACTGCGTTTTTCATCCGGCGAGCCGGTACGCATCAGCGGTGCAACGTTCAGCGtt                         >  1:247334/1‑250 (MQ=255)                                                                                                                                                                                                                              gTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTgg                   >  1:259552/1‑250 (MQ=255)                                                                                                                                                                                                                              gTCCGT‑TTTTCCCGGCAATTGGTTCGACAACCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATCCACGCCCGCGTCGAGGATACGGCTTTCATCCGTTATCTGATCGAGGATCGCCTGAATGCGTTGTTCATCCGTCGAGTCGGTACGCATCAGCGGTGCAACTTTCAGCGTTACCTGa                   <  1:331406/251‑1 (MQ=255)                                                                                                                                                                                                                                         cccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAgg          <  1:130340/246‑1 (MQ=255)                                                                                                                                                                                                                                                aTTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGc  >  1:96470/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGC  >  NC_000913/4624157‑4624645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑