Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,391	C→G	A133A (GCG→GCC)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,391	0	C	G	100.0%	69.7 / NA	24	A133A (GCG→GCC)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (13/11);  total (13/11)

CTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGA  >  NC_000913/4624150‑4624628                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              ctgtgttcccGCTTTCTTCCTCTTCCGTCACCGGTTTGCTGGCGGTTAACAGGGAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTTCGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTg                                                                                                                                                                                                                                        <  2:120173/240‑1 (MQ=255)        ccggCTTTCTCCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGAGTGCTGCCAGCGGGTTCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACaa                                                                                                                                                                                                                               <  2:129525/248‑1 (MQ=255)         tcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTTCCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGGTTGCCAGTGAAAGCAGCAACATCCGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGGGCGTCAGGCGCAGATAGCCGCGCGGGCCGG‑TTTTCCCGGCGATTGGTTCGACAAt                                                                                                                                                                                                                              >  1:1916/1‑251 (MQ=255)                cttcctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGGGCGGGTCCGCACTACGCCGATTGCCAGTGAAAGCAGCCACATCAGGCGTAAAATGTTAGGGGTGTTATCCACCTGTTGGGGTTCGGTGGCGAGCGTATGGGGGACGAGCGTCAGGCGCAGATAACCGAGCGGTCCGG‑TTTTCCCGGCAAATGGGTCGACAATCTGCTgg                                                                                                                                                                                                                       >  1:165534/1‑251 (MQ=255)                        ccgggCATCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTTCGTTTACCCGGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTGAAAATa                                                                                                                                                                                                               <  2:165534/248‑1 (MQ=255)                               ccGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAgccgccc                                                                                                                                                                                                        <  1:195674/251‑2 (MQ=255)                                                                gctgcCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTcgcg                                                                                                                                                                        <  1:26192/250‑1 (MQ=255)                                                                                                                            gTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCcgcg                                                                                                           >  1:141239/1‑251 (MQ=255)                                                                                                                              gaaagcagcAACATCAGGCGTAAAATGTTAGTGGTGTTATCCCCCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTTGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTc                                                                                                         <  1:13240/248‑1 (MQ=255)                                                                                                                                    agcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGAt                                                                                                   <  1:26266/251‑1 (MQ=255)                                                                                                                                     gcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGAt                                                                                                   >  1:222245/1‑250 (MQ=255)                                                                                                                                              ggCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTc                                                                                          >  1:336467/1‑250 (MQ=255)                                                                                                                                                       tGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc                                                                                >  1:324571/1‑251 (MQ=255)                                                                                                                                                        gtgtgtgggtgtctcctccttttGGGCGTCGGTGGCGGGCGTATGGGTGTCGAGCGGTAGGCGCAGATAGCCGAGCGGTC‑GTGTTTTCCCGGCAATGGGTGCGACAATCTGCTGGTGAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACGTCGACGCTTTCGCCAGAACGGGCGATAAGATCTCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAAc                                                                                <  2:136968/229‑1 (MQ=255)                                                                                                                                                                   tATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGAGTAAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGGTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCCATTCGACGCTTTCGCCAGAACGGGCGATAAGGTCGACTTGTTCGTCATACACGCCCGCCGCGAGGTTACGGATTTCATCCGTTTACTGCACGAGGagc                                                                    >  2:106966/1‑249 (MQ=255)                                                                                                                                                                        cacgtttGGGCTTCGGGTGCGGGCGTATGGGTGGCGAGCGGCAGGTGCCGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCTCTTTTTTACCGTCGGGCGCCTGCCGGTCGCGCACTTCGACTCTTTCGCCATAACGGGCGATAAGATCGCCTTGGTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTg                                                               <  2:193796/246‑1 (MQ=255)                                                                                                                                                                         ccTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGa                                                              <  1:246161/251‑1 (MQ=255)                                                                                                                                                                               gggCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATCAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGt                                                        >  1:158659/1‑251 (MQ=255)                                                                                                                                                                                   ttCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGttttt                                                    >  1:307536/1‑251 (MQ=255)                                                                                                                                                                                                  aTGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCgg                                     >  1:271081/1‑251 (MQ=255)                                                                                                                                                                                                               gtCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCgg                        >  1:150564/1‑251 (MQ=255)                                                                                                                                                                                                                               cGAGCGGTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGCTACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGACTGCGTTTTTCATCCGGCGAGCCGGTACGCATCAGCGGTGCAACGTTCAGCGtt        >  1:247334/1‑250 (MQ=255)                                                                                                                                                                                                                                     gTCCGT‑TTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTgg  >  1:259552/1‑250 (MQ=255)                                                                                                                                                                                                                                     gTCCGT‑TTTTCCCGGCAATTGGTTCGACAACCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATCCACGCCCGCGTCGAGGATACGGCTTTCATCCGTTATCTGATCGAGGATCGCCTGAATGCGTTGTTCATCCGTCGAGTCGGTACGCATCAGCGGTGCAACTTTCAGCGTTACCTGa  <  1:331406/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              CTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGT‑TTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGA  >  NC_000913/4624150‑4624628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑