Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,277	G→A	L171L (CTC→CTT)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,277	0	G	A	100.0%	76.1 / NA	28	L171L (CTC→CTT)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCG  >  NC_000913/4624030‑4624523                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       cGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGGCAGCACTACGCCGGTTGCCCGTGAAagc                                                                                                                                                                                                                                                     >  1:136968/1‑251 (MQ=255)   ggCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAagcag                                                                                                                                                                                                                                                   <  1:294826/251‑1 (MQ=255)        cgggctaaatatccactcctgcACCTCCAGTTTAAACCACTGGTCGTCTGAGTCAGAGGTGAGCAGGCGTAATGTGGACATAATGACTGCCTTTCTTGTAGTAATTGGTCACTCTTTTCTCTCTCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGGGAACAGGAAGGGCGATTGCTGCCAGCGGGTTCGTTTTCCCTGTAGCAGC‑GTGCTGGTCGGCACTTCGCCGATTTCCAGTGAAAGCAGCAACa                                                                                                                                                                                                                                              <  2:293195/230‑1 (MQ=255)              aaaaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGc                                                                                                                                                                                                                                        <  1:24036/251‑1 (MQ=255)              aaaaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGACGAGCAGCCGTAATGTGGACATAACGATTTCCTTTCTTGAAGTCATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAAAGGGAGGGCGATTGCTTCCCACGGGCACGCTTACCCTGTACCAACAGTG‑GGGACAGCACTATGCCGAATGCCACTGAAagcagacaaaaacggc                                                                                                                                                                                                                                        >  1:178749/1‑240 (MQ=255)               aaaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCg                                                                                                                                                                                                                                       <  1:103993/251‑1 (MQ=255)                aaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGt                                                                                                                                                                                                                                      <  1:295635/251‑1 (MQ=255)                             cACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGGTAACAGGAAGGGCGCATGATGCCAGCGGGGACGGTTTCCCTGTGGCAGC‑GAGAGGGTCCGCACAACGCCGAGTGCCAGTTAAAGCAGCcacacacagcggaaaatgtgttggg                                                                                                                                                                                                                         >  2:13240/1‑230 (MQ=255)                                           aCCACGGGTCGTAAGAGTCAGAGATGAGAAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAAGGCGATTGCTGCCCGCGGGTACGGTTAACCCGGAGCAGA‑GTGCGGGGCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACCTCAGGCGGAAAATGTTCGTGGTGTTATCCCCCTGt                                                                                                                                                                                                           >  1:267172/1‑251 (MQ=255)                                                                   tGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAAAGGAAGGGCGATTGCTGCCAGCGGGTAAGTTTACCCTGTAGCAGC‑GTGCGGGGCAGCACTAAGCCGATTGCCCGTGAAAGCAGCAACATCAGGCGTAAAATTTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATgg                                                                                                                                                                                   >  1:193796/1‑251 (MQ=255)                                                                   ggAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATgg                                                                                                                                                                                   <  1:150865/250‑1 (MQ=255)                                                                           cGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAg                                                                                                                                                                           <  1:58377/251‑1 (MQ=255)                                                                                          aCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCCGGCGTAAAATGTTAGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGTGTCGAGGGTCAGGCGCCGAt                                                                                                                                                             >  1:25843/1‑250 (MQ=255)                                                                                              tttCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTAACCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCCGAGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGGGCGTATGGGGGTCGAGCGTCAGGCGCAGATAGCCg                                                                                                                                                        >  1:211911/1‑251 (MQ=255)                                                                                                        ttttgtaATGGGTCCCTCTTTTTTCCCGCTTTCTGCTTCTTCCGGCACTGGTTTGCTGGCGGTTAACAGGAATGGAGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCTCCTGTTGGGCTTGGGTGGCGAGCGTATGGGTGTCTAGCGTCAGGCGCAGATAGCCGAGCGGTcc                                                                                                                                                <  2:143663/245‑1 (MQ=255)                                                                                                        tGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGCTTTCTTCCCGCGGGGACGTTTTCCCTTTAGCAGC‑GTGCGGGGCAGCACTAAGCCCATTGCCAGTGAAAGCCGCAACAACAGGCGTAAAATGTTAGGGGTGGTTTCCCCCTGTTTGGGTTTGGTGGGGAGCGTATGGGGGGCGAGGGTCAGgcgctgggaggcgggcgggccgg                                                                                                                                              >  2:195674/1‑232 (MQ=255)                                                                                                           gttaaggtctcctcctTTTGTCTCCCTTTCTTCCTCTTCCTGCACCGGCTTGCTGGCGTTTCACAGGTATGGCTATTGCTTCCAGCGGGTACGTTTTCCCTGTATCAGC‑GTGCGGGTCAGCGCCACGCAGATGTCCAGTGAACGCAGCATCATCAGGCGTAAAATGTTAGTGGTGTTATCCTCCTGTTGGGCTTTGGTGGCGAGTGTATGGGTGTCGAGCGTCAGGTGCAGATTGCCGTGCGTTCCGtttt                                                                                                                                           <  2:214738/237‑1 (MQ=255)                                                                                                                  ggTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGAAGTTTACCCTGTAGCAGC‑GGGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACCTCCGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGTGTCGGGCGTCAGGCGCAGATAGCCGGGCGGTCCGttttttccgac                                                                                                                                    >  1:245741/1‑245 (MQ=255)                                                                                                                         ctgtgttcccGCTTTCTTCCTCTTCCGTCACCGGTTTGCTGGCGGTTAACAGGGAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTTCGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTg                                                                                                                               <  2:120173/240‑1 (MQ=255)                                                                                                                              ttCTCGCTTTCTTCCTCTTCCGGCCCCGGTTTGCTGGCGGGTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGtttttcccgg                                                                                                                                     <  2:296068/238‑6 (MQ=255)                                                                                                                              ttCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGGAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGGGTCGAGCGTCCGGCGCAGATAGCCGGGCGGGCCGtttttcccggggatcgggagagg                                                                                                                        >  1:296068/1‑233 (MQ=255)                                                                                                                                ccggCTTTCTCCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGAGTGCTGCCAGCGGGTTCGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACaa                                                                                                                      <  2:129525/248‑1 (MQ=255)                                                                                                                                 tcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTTCCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGGTTGCCAGTGAAAGCAGCAACATCCGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGGGCGTCAGGCGCAGATAGCCGCGCGGGCCGGTTTTCCCGGCGATTGGTTCGACAAt                                                                                                                     >  1:1916/1‑251 (MQ=255)                                                                                                                                        cttcctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GGGCGGGTCCGCACTACGCCGATTGCCAGTGAAAGCAGCCACATCAGGCGTAAAATGTTAGGGGTGTTATCCACCTGTTGGGGTTCGGTGGCGAGCGTATGGGGGACGAGCGTCAGGCGCAGATAACCGAGCGGTCCGGTTTTCCCGGCAAATGGGTCGACAATCTGCTgg                                                                                                              >  1:165534/1‑251 (MQ=255)                                                                                                                                                ccgggCATCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTTCGTTTACCCGGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTGAAAATa                                                                                                      <  2:165534/248‑1 (MQ=255)                                                                                                                                                       ccGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAgccgccc                                                                                               <  1:195674/251‑2 (MQ=255)                                                                                                                                                                                        gctgcCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTcgcg                                                               <  1:26192/250‑1 (MQ=255)                                                                                                                                                                                                                                                     gTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCcgcg  >  1:141239/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCG  >  NC_000913/4624030‑4624523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑