Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,262	A→G	G176G (GGT→GGC)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,262	0	A	G	100.0%	70.2 / NA	32	G176G (GGT→GGC)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCG  >  NC_000913/4624015‑4624462                                                                                                                                                                                                                                                         |                                                                                                                                                                                                         gAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCg                                                                                                                                                                                                       <  1:62461/251‑1 (MQ=255)   actcgcTGCGGGGCGGGCATATGGCGAAAAATACACTCCGCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTGCTCGCGTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGAt                                                                                                                                                                                                     <  1:371455/248‑1 (MQ=255)          gCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCa                                                                                                                                                                                                <  1:236874/249‑1 (MQ=255)                cGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGGCAGCACTACGCCGGTTGCCCGTGAAagc                                                                                                                                                                                        >  1:136968/1‑251 (MQ=255)                  ggCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAagcag                                                                                                                                                                                      <  1:294826/251‑1 (MQ=255)                       cgggctaaatatccactcctgcACCTCCAGTTTAAACCACTGGTCGTCTGAGTCAGAGGTGAGCAGGCGTAATGTGGACATAATGACTGCCTTTCTTGTAGTAATTGGTCACTCTTTTCTCTCTCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGGGAACAGGAAGGGCGATTGCTGCCAGCGGGTTCGTTTTCCCTGTAGCAGC‑GTGCTGGTCGGCACTTCGCCGATTTCCAGTGAAAGCAGCAACa                                                                                                                                                                                 <  2:293195/230‑1 (MQ=255)                             aaaaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGc                                                                                                                                                                           <  1:24036/251‑1 (MQ=255)                             aaaaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGACGAGCAGCCGTAATGTGGACATAACGATTTCCTTTCTTGAAGTCATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAAAGGGAGGGCGATTGCTTCCCACGGGCACGCTTACCCTGTACCAACAGTG‑GGGACAGCACTATGCCGAATGCCACTGAAagcagacaaaaacggc                                                                                                                                                                           >  1:178749/1‑240 (MQ=255)                              aaaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCg                                                                                                                                                                          <  1:103993/251‑1 (MQ=255)                               aaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGt                                                                                                                                                                         <  1:295635/251‑1 (MQ=255)                                            cACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGGTAACAGGAAGGGCGCATGATGCCAGCGGGGACGGTTTCCCTGTGGCAGC‑GAGAGGGTCCGCACAACGCCGAGTGCCAGTTAAAGCAGCcacacacagcggaaaatgtgttggg                                                                                                                                                            >  2:13240/1‑230 (MQ=255)                                                          aCCACGGGTCGTAAGAGTCAGAGATGAGAAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAAGGCGATTGCTGCCCGCGGGTACGGTTAACCCGGAGCAGA‑GTGCGGGGCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACCTCAGGCGGAAAATGTTCGTGGTGTTATCCCCCTGt                                                                                                                                              >  1:267172/1‑251 (MQ=255)                                                                                  tGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAAAGGAAGGGCGATTGCTGCCAGCGGGTAAGTTTACCCTGTAGCAGC‑GTGCGGGGCAGCACTAAGCCGATTGCCCGTGAAAGCAGCAACATCAGGCGTAAAATTTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATgg                                                                                                                      >  1:193796/1‑251 (MQ=255)                                                                                  ggAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATgg                                                                                                                      <  1:150865/250‑1 (MQ=255)                                                                                          cGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAg                                                                                                              <  1:58377/251‑1 (MQ=255)                                                                                                        aaCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GGGCGGGGCAGCAATACGCCGAtt                                                                                                                                                                                                    >  1:230215/1‑151 (MQ=255)                                                                                                        aaCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCGCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGTGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGAtt                                                                                                                                                                                                    <  2:230215/151‑1 (MQ=255)                                                                                                         aCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCCGGCGTAAAATGTTAGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGTGTCGAGGGTCAGGCGCCGAt                                                                                                >  1:25843/1‑250 (MQ=255)                                                                                                             tttCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTAACCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCCGAGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGGGCGTATGGGGGTCGAGCGTCAGGCGCAGATAGCCg                                                                                           >  1:211911/1‑251 (MQ=255)                                                                                                                       ttttgtaATGGGTCCCTCTTTTTTCCCGCTTTCTGCTTCTTCCGGCACTGGTTTGCTGGCGGTTAACAGGAATGGAGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCTCCTGTTGGGCTTGGGTGGCGAGCGTATGGGTGTCTAGCGTCAGGCGCAGATAGCCGAGCGGTcc                                                                                   <  2:143663/245‑1 (MQ=255)                                                                                                                       tGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGCTTTCTTCCCGCGGGGACGTTTTCCCTTTAGCAGC‑GTGCGGGGCAGCACTAAGCCCATTGCCAGTGAAAGCCGCAACAACAGGCGTAAAATGTTAGGGGTGGTTTCCCCCTGTTTGGGTTTGGTGGGGAGCGTATGGGGGGCGAGGGTCAGgcgctgggaggcgggcgggccgg                                                                                 >  2:195674/1‑232 (MQ=255)                                                                                                                          gttaaggtctcctcctTTTGTCTCCCTTTCTTCCTCTTCCTGCACCGGCTTGCTGGCGTTTCACAGGTATGGCTATTGCTTCCAGCGGGTACGTTTTCCCTGTATCAGC‑GTGCGGGTCAGCGCCACGCAGATGTCCAGTGAACGCAGCATCATCAGGCGTAAAATGTTAGTGGTGTTATCCTCCTGTTGGGCTTTGGTGGCGAGTGTATGGGTGTCGAGCGTCAGGTGCAGATTGCCGTGCGTTCCGtttt                                                                              <  2:214738/237‑1 (MQ=255)                                                                                                                                 ggTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGAAGTTTACCCTGTAGCAGC‑GGGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACCTCCGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGTGTCGGGCGTCAGGCGCAGATAGCCGGGCGGTCCGttttttccgac                                                                       >  1:245741/1‑245 (MQ=255)                                                                                                                                        ctgtgttcccGCTTTCTTCCTCTTCCGTCACCGGTTTGCTGGCGGTTAACAGGGAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTTCGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTg                                                                  <  2:120173/240‑1 (MQ=255)                                                                                                                                             ttCTCGCTTTCTTCCTCTTCCGGCCCCGGTTTGCTGGCGGGTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGtttttcccgg                                                                        <  2:296068/238‑6 (MQ=255)                                                                                                                                             ttCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGGAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGGGTCGAGCGTCCGGCGCAGATAGCCGGGCGGGCCGtttttcccggggatcgggagagg                                                           >  1:296068/1‑233 (MQ=255)                                                                                                                                               ccggCTTTCTCCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGAGTGCTGCCAGCGGGTTCGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACaa                                                         <  2:129525/248‑1 (MQ=255)                                                                                                                                                tcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTTCCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGGTTGCCAGTGAAAGCAGCAACATCCGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGGGCGTCAGGCGCAGATAGCCGCGCGGGCCGGTTTTCCCGGCGATTGGTTCGACAAt                                                        >  1:1916/1‑251 (MQ=255)                                                                                                                                                       cttcctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GGGCGGGTCCGCACTACGCCGATTGCCAGTGAAAGCAGCCACATCAGGCGTAAAATGTTAGGGGTGTTATCCACCTGTTGGGGTTCGGTGGCGAGCGTATGGGGGACGAGCGTCAGGCGCAGATAACCGAGCGGTCCGGTTTTCCCGGCAAATGGGTCGACAATCTGCTgg                                                 >  1:165534/1‑251 (MQ=255)                                                                                                                                                               ccgggCATCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTTCGTTTACCCGGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTGAAAATa                                         <  2:165534/248‑1 (MQ=255)                                                                                                                                                                      ccGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAgccgccc                                  <  1:195674/251‑2 (MQ=255)                                                                                                                                                                                                       gctgcCAGCGGGTACGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTcgcg  <  1:26192/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                         GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGC‑GTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCG  >  NC_000913/4624015‑4624462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑