Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,623,983 T→C V45V (GTA→GTG)  lplA ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,623,9830TC94.6% 105.1 / ‑2.6 38V45V (GTA→GTG) lplAlipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (18/17);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

CACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGATTGCTGCCAGCGGGTG  >  NC_000913/4623740‑4624226
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
caccacCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGCGATGGTTTTATCGTACTCCGGCTTGACAGCCATAAAAGTAACGCAGGTATTTACGAGCTCGTGGCACACCGAACCGCCACAGCTACTGCGCCGTGCCAGGCGGACGTTATCTTATTCCATCCGCCGGGTCTTACACTCTTTACACGGGTTCTGTGCGCGACCAATCACCACCg                                                                                                                                                                                                                                               >  2:319074/1‑251 (MQ=255)
 cccaccaGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCgt                                                                                                                                                                                                                                              <  1:191468/250‑1 (MQ=255)
         aTCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGGGTCGGCAt                                                                                                                                                                                                                                      >  1:282304/1‑251 (MQ=255)
                              ggcacTGACGCCAAGCGCATTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCAAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCAGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCATCCGGGTATTACACTCTTTCCACGGGTTCTGCGAGCAACAAAACACTACCGTGTCGACATTACGCCAGAGAAACaacacac                                                                                                                                                                                                                 >  2:62461/5‑245 (MQ=255)
                                  cTGACGCCAAGCGCGGTAAACGCATTGAGCACAATCGACGTGGAGATGGTTATATCGTCCTCCGGCTTGCCGGCCATAAAGGTAAATCAGGTATTTCCGAGATCGTGGAACACCGCGCCGCCACCGCGACTGCACCCTGCCAGGCGGCCGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTGCTGCGCGCAACCAATACCTGCCGTTTCGGCATTTCGCCCGAGAAACAGCACgcgcgc                                                                                                                                                                                                             >  2:150865/1‑249 (MQ=255)
                                     aCGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGt                                                                                                                                                                                                          >  1:10600/1‑251 (MQ=255)
                                       gccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTgg                                                                                                                                                                                                        <  1:128382/247‑1 (MQ=255)
                                        ccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGc                                                                                                                                                                                                       >  1:143663/4‑251 (MQ=255)
                                             cgcgTTAAGCGCATTGAGCCCAATCGACGTGGTGATGGTTTTGTCGTTCTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACGACCGTGTCGGCATTGCGCCAGAGAAACAGAACGAGCTGCGTGGCGGGCa                                                                                                                                                                                                  <  2:113825/251‑1 (MQ=255)
                                                               cacaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACAct                                                                                                                                                                                >  1:240812/1‑251 (MQ=255)
                                                               cacaAGCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACAct                                                                                                                                                                                <  1:176096/251‑1 (MQ=255)
                                                                acaATCGACGTGGAGATGGTTTTATCGTAATCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACActc                                                                                                                                                                               >  1:111669/1‑251 (MQ=255)
                                                                             ggatggTTTTATCGTCCCCAGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACTGCGCCGTGCCATGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAAGCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAgg                                                                                                                                                                  <  2:10600/246‑1 (MQ=255)
                                                                              gctggTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCACTGCGTGGCGGGGCTCTGGCGGAAAATAAACTCCTCCACCGCCAGGt                                                                                                                                                                 >  2:5825/5‑251 (MQ=255)
                                                                                       aTCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACg                                                                                                                                                        >  1:241110/1‑251 (MQ=255)
                                                                                                cGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAag                                                                                                                                               <  1:9489/251‑1 (MQ=255)
                                                                                                                aaGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCAAGGGTCGTAAGAGTCAGAGATGAGc                                                                                                                                 >  1:167809/1‑249 (MQ=255)
                                                                                                                aaGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTACACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAg                                                                                                                               <  1:162065/251‑1 (MQ=255)
                                                                                                                       aGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGAGGAGCAGGCGTAAt                                                                                                                        <  1:77052/251‑1 (MQ=255)
                                                                                                                            gTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                                                                                   <  1:61293/251‑1 (MQ=255)
                                                                                                                                                   cgctccgccACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCACCACCGCCAGGTTAAACCACGGGTCGTCAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTACTTTCTTgt                                                                                              >  1:263212/5‑249 (MQ=255)
                                                                                                                                                     cgccgcCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                                                                                          <  1:319074/251‑1 (MQ=255)
                                                                                                                                                            aCCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTc                                                                                   <  1:242157/251‑1 (MQ=255)
                                                                                                                                                              cGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACTATTTCCTTTCTTGTAGTAATGGGTCAc                                                                                 <  1:298445/251‑1 (MQ=255)
                                                                                                                                                              cGCTACTGCGCCGTGACAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGACACAGAACGCGCTGCGTGGCGGGCCTCTGGCGCAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGGGTCAGAGATGTGCAGGCGTAATGTGGACATAAAGATTTCCTTTTTTGTTGTAATTGGTCAc                                                                                 >  2:26266/1‑251 (MQ=255)
                                                                                                                                                                         cgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGCAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTGGTAATGGGTCACTCTTTTTtctc                                                                      >  1:214738/4‑251 (MQ=255)
                                                                                                                                                                          gtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCGTGTAGTAATGGGTCACTCTTTTTTCTCg                                                                     <  1:117509/249‑1 (MQ=255)
                                                                                                                                                                               aGGCGGATGTTTACTTCTTCCCACCTCCGGGTATTACCCCCTTTACACGGGTTCTGCGCTCGACCAATTACTACCGTGTCGGCATTGCGCCAGCGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTATTAATGGGTCACCCTTTTTTCTCGCttt                                                                 <  2:72714/250‑1 (MQ=255)
                                                                                                                                                                                   ggACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcc                                                            <  1:5825/251‑1 (MQ=255)
                                                                                                                                                                                                    aaccgccgGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                                                                                 <  2:307027/179‑1 (MQ=255)
                                                                                                                                                                                                    aTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                                                                                 >  1:307027/1‑181 (MQ=255)
                                                                                                                                                                                                        gccgGGTATTTCAATCTGTCCCCGGGTTCGGCGCGCGACCAATCACTCCCGTGTCGGCATGGCGCCCGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCTCCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGTCATAACGATTTCCTTTCTTGTAGTAATGGGTCTCTCTTTTTTCTCGCTTTCTTCCTCATCCGGCACCGGTTTGCTg                                       <  2:25167/251‑1 (MQ=255)
                                                                                                                                                                                                               aTTACACTCTTACCACGGGTTATGCGCGCGCCCAATCACTACCTTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCTTCTGGAGAAAAACACAATCCTCCACCGCCCGGGTAAACCACAGGTCAGAAAAAACAGAGAAACGCACGCGTAATATGGAACTAAAGATTTCATTTTTTGTAGGTATGGGACACTCTTTTTTCTCGCCTTCTTCATCATACGGACCCGGTTTGCTGGCGG‑t                                 >  2:246161/1‑249 (MQ=255)
                                                                                                                                                                                                                            cACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCGCCACCGCCAGGTTAAACCACGGGTCGTAAGATTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGa                  <  1:29831/251‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTCTGCACGAGACCAATCACTACAGTGTCGCCATAGCGACAGCGAAACAGAACGCGCTACGTGGCCGGCATCTGGCGAAAAATACACTCATCCCACGACGGGGTACACCCCGGGAAGTAAGAGAAAGACATGAGCAGGCGAAATGTAGAAATACCGCATTCCTTTCTTGACGTAATAGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTTCGGCACCAGTATGCTGGCGG‑tatacaagacaggagaatttg             >  2:332829/1‑231 (MQ=255)
                                                                                                                                                                                                                                            ggccAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACAGCCAGGTTAAACCACGGGGCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTTTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGGTGGCGGATTAACAG‑AAGGGGGATTGTTGCCAGgggggg  >  2:130340/3‑249 (MQ=255)
                                                                                                                                                                                                                                             aCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAt                                                                                        >  2:127105/1‑165 (MQ=255)
                                                                                                                                                                                                                                             aCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAt                                                                                        <  1:127105/165‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
CACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGATTGCTGCCAGCGGGTG  >  NC_000913/4623740‑4624226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑