Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,821	A→G	T99T (ACT→ACC)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,821	0	A	G	92.3%	109.7 / ‑3.7	39	T99T (ACT→ACC)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (18/18);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTG‑GAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACG‑GTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTC  >  NC_000913/4623576‑4624054                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          actgaggTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGtttt                                                                                                                                                                                                                                      <  1:97883/245‑1 (MQ=255) aCCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTG‑GAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGACGCCTTCGACG‑GTTTTCACCACCAGCTCGTTACGTCAGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTTAGCCAAATCGACGAGGAGATAGtttt                                                                                                                                                                                                                                      >  2:162065/1‑251 (MQ=255)   tggggTAATGCCTTTCTCCTCCAGGTGCTTTTTATCCGGATTGCGATAGTTTGCCCGGCTGCTAAGGTCGGCATTGAGCATACAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGTTCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTTGAGATGGtttt                                                                                                                                                                                                                                      <  2:226441/245‑1 (MQ=255)                       ccAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTgccagcc                                                                                                                                                                                                                >  1:39410/1‑251 (MQ=255)                         aGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTG‑GAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACG‑GTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCACGTTCAGCGCATTGAGCACAATCGCCGTGGAGATAGCTTTATCGTACTCCGGCTTgccagccc                                                                                                                                                                                                               >  2:141610/1‑249 (MQ=255)                                        ggATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTTAGCACCATCGACGTGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAggg                                                                                                                                                                                               >  1:113825/1‑250 (MQ=255)                                                     ttGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTTTCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGccc                                                                                                                                                                                        >  1:25167/1‑244 (MQ=255)                                                       gCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGTCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTAATCCGGCTTGCCAGACATAAAGGTAAAGCAGGTATTTCCGAGATCGt                                                                                                                                                                                 <  1:90585/250‑1 (MQ=255)                                                                  aaGGTCGGCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACAcc                                                                                                                                                                         <  1:273479/246‑1 (MQ=255)                                                                  aaGGTCGGCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGCTTAGGGCCTTGAGCACAAACGCCGTGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAACACGTATTGCCggagtcgtggaccaacagct                                                                                                                                                                     >  2:273479/2‑232 (MQ=255)                                                                        ggCATTGAGCAGCAAGGTGCCGTGGTG‑GAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑CTTTTCACCACCAGATCGTTACGCCCGGACGCTTCCGCGCTGACGCCAAGCGCGTTCAGCGCCTTCAGAACCATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTTCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACAACGCGCCGCCAc                                                                                                                                                               >  1:381936/1‑251 (MQ=255)                                                                         gaaTTGAGCACCAAGGTGGCG‑GGTGTGAAGCCACGTTATTTGGTTTCGCGATAGGCCTATCCTGAGACTTTGCGGTCGCCGTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACTCTTCGGCGCGTACGACAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTATTCTGTCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGTGATCGTGGAACACCCCGCCGCCAcc                                                                                                                                                              <  2:251072/249‑1 (MQ=255)                                                                                                  ggtaggccacgATCTTTGGTTTCGGGATAGGCCGAGCCTGAGACTGTGCGGCCGCCTGCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGTCGCTTCGGCGCTGACGCCAAGCGCGGTCAGCGCATTGAGCTCAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAATGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCAGTGCCAGGCgtac                                                                                                                                      <  2:27255/242‑4 (MQ=255)                                                                                                     aaGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGtt                                                                                                                                   <  1:290664/251‑1 (MQ=255)                                                                                                           cgATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGACTCGGCGCTGACGCCCAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGAATTTCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTAtattc                                                                                                                             >  1:293195/1‑251 (MQ=255)                                                                                                                 ttGGTTTCGCGATAGGCCGATCCTGAGACTGTGCTGTCGCCTGCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGGTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATcttctt                                                                                                                           <  1:184171/247‑1 (MQ=255)                                                                                                                     tttCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATc                                                                                                                                                                                   <  1:327834/187‑1 (MQ=255)                                                                                                                     tttCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAAGCGGACGTTATCTTCTTCCATccgc                                                                                                                   >  1:120173/1‑251 (MQ=255)                                                                                                                        cgcgATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCCAATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGAATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCCCCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCAACCGCCgg                                                                                                                >  1:185510/1‑251 (MQ=255)                                                                                                                              aGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTACCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTa                                                                                                          <  1:129295/251‑1 (MQ=255)                                                                                                                                gCCGATCCTGAGACTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAacac                                                                                                                                                                          <  1:303257/185‑1 (MQ=255)                                                                                                                                     gCCTGAGACTTTGCGGTCGCCTTCGACG‑GTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCACGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGCTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCtt                                                                                                   <  1:141610/251‑1 (MQ=255)                                                                                                                                             cTTTGCGGTCGCCTTCGGCG‑GTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACggg                                                                                           >  1:79375/1‑251 (MQ=255)                                                                                                                                                 gCGGTCGCTTTCG‑CGTGTTTTCACCTCCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCTTTCAGCGCCGTGAGCACAATCGACGTGGAGATGGTTTTATCTTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGTTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCGCGGGTTCt                                                                                       <  2:105012/251‑1 (MQ=255)                                                                                                                                                                  gTTTTCACCGCCGGAGCGTTAGGCCCGGGCGCTTCGGCGCTGGCGCCAAGCGGGTTCAGCGGATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCTCGCGACCAAtcac                                                                       <  1:324575/251‑4 (MQ=255)                                                                                                                                                                       caccacCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGCGATGGTTTTATCGTACTCCGGCTTGACAGCCATAAAAGTAACGCAGGTATTTACGAGCTCGTGGCACACCGAACCGCCACAGCTACTGCGCCGTGCCAGGCGGACGTTATCTTATTCCATCCGCCGGGTCTTACACTCTTTACACGGGTTCTGTGCGCGACCAATCACCACCg                                                                  >  2:319074/1‑251 (MQ=255)                                                                                                                                                                        cccaccaGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCgt                                                                 <  1:191468/250‑1 (MQ=255)                                                                                                                                                                              aGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAgg                                                                                                                                                                                                >  1:345983/1‑118 (MQ=255)                                                                                                                                                                              aGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCAGAAAGGTAAAGCAgg                                                                                                                                                                                                <  2:345983/118‑1 (MQ=255)                                                                                                                                                                                aTCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGGGTCGGCAt                                                         >  1:282304/1‑251 (MQ=255)                                                                                                                                                                                                     ggcacTGACGCCAAGCGCATTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCAAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCAGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCATCCGGGTATTACACTCTTTCCACGGGTTCTGCGAGCAACAAAACACTACCGTGTCGACATTACGCCAGAGAAACaacacac                                    >  2:62461/5‑245 (MQ=255)                                                                                                                                                                                                         cTGACGCCAAGCGCGGTAAACGCATTGAGCACAATCGACGTGGAGATGGTTATATCGTCCTCCGGCTTGCCGGCCATAAAGGTAAATCAGGTATTTCCGAGATCGTGGAACACCGCGCCGCCACCGCGACTGCACCCTGCCAGGCGGCCGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTGCTGCGCGCAACCAATACCTGCCGTTTCGGCATTTCGCCCGAGAAACAGCACgcgcgc                                >  2:150865/1‑249 (MQ=255)                                                                                                                                                                                                            aCGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGt                             >  1:10600/1‑251 (MQ=255)                                                                                                                                                                                                              gccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTgg                           <  1:128382/247‑1 (MQ=255)                                                                                                                                                                                                               ccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGc                          >  1:143663/4‑251 (MQ=255)                                                                                                                                                                                                                    cgcgTTAAGCGCATTGAGCCCAATCGACGTGGTGATGGTTTTGTCGTTCTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACGACCGTGTCGGCATTGCGCCAGAGAAACAGAACGAGCTGCGTGGCGGGCa                     <  2:113825/251‑1 (MQ=255)                                                                                                                                                                                                                                      cacaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACAct   >  1:240812/1‑251 (MQ=255)                                                                                                                                                                                                                                      cacaAGCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACAct   <  1:176096/251‑1 (MQ=255)                                                                                                                                                                                                                                       acaATCGACGTGGAGATGGTTTTATCGTAATCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACActc  >  1:111669/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          ACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTG‑GAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACG‑GTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTC  >  NC_000913/4623576‑4624054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑