Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,623,502 C→T E206K (GAG→AAG)  lplA ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,623,5020CT92.6% 72.1 / ‑1.4 29E206K (GAG→AAG) lplAlipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (20/5);  total (21/8)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

AGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACC  >  NC_000913/4623261‑4623746
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
aGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGc                                                                                                                                                                                                                                              >  1:226441/1‑251 (MQ=255)
       cGGTAAACACATGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCa                                                                                                                                                                                                                                        >  1:36506/1‑250 (MQ=255)
               acCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTc                                                                                                                                                                                                                               >  1:130535/1‑251 (MQ=255)
                ccTGGGCGCGGGTGATATAGCCTATTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACATGCta                                                                                                                                                                                                                               >  1:154240/1‑249 (MQ=255)
                      cgcgGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTg                                                                                                                                                                                                                        <  1:191277/251‑1 (MQ=255)
                                                       gCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCAc                                                                                                                                                                                        <  1:104061/250‑1 (MQ=255)
                                                                cgccgcCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGGCACGCGGGaccgc                                                                                                                                                                              >  2:217510/1‑247 (MQ=255)
                                                                cgccgcCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTACATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGATTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGATGGTCACGCGGGAACgc                                                                                                                                                                              >  1:55182/1‑250 (MQ=255)
                                                                  ccgccCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgcac                                                                                                                                                                            <  1:217510/251‑4 (MQ=255)
                                                                     ccccAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGAACGAAGATCCATTCCCAGCTACTCTGACGGGCAACGGATTCGGCGAAGTTTGGCACGTCTGACGTTTTGTCCGGGGCAATGATTTCCGCTTCTACACACTCGCCGTAATGGCCGAAAAGGGCCTTGGTTATGTCCTCGCAAACCTGCTCATGGGGGATCCCCGGACGCAGCTCGGTTCGTATGGTCACGCGGGGACgcattgg                                                                                                                                                                         >  2:176096/1‑245 (MQ=255)
                                                                           ctAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AAt                                                                                                                                                                  >  1:205750/2‑251 (MQ=255)
                                                                               aGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCt                                                                                                                                                              >  1:163005/1‑251 (MQ=255)
                                                                                           gtaggtGCGAGAATGCAGGAGCCTGGCCGAGGTTCCAGTCCCAGATACTCTGACGGGAAAAGGTTGCGGGGAAGTGGGGCAAGTCTGGCGTTTTGTCCGGGAAGATGATTTCCGTGTCAACACGCTCTCCATAATGGGCGAAAAAGGCCTGGGTTAGGGCCTCGCAACCCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGt                                                                                                                                                  <  2:193712/246‑1 (MQ=255)
                                                                                             agATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGttt                                                                                                                                                >  1:150795/1‑251 (MQ=255)
                                                                                              gATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCCAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGATCGGGGAGGTTGGTACCGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCCGTTTc                                                                                                                                               >  1:32411/1‑251 (MQ=255)
                                                                                                                                 tCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCt                                                                                                            >  1:369636/1‑251 (MQ=255)
                                                                                                                                  cccAGCTACTCTGACGGGCAAAGGTTTCGGAGAAATTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATCATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑CAGGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCCGGCGGCt                                                                                                            >  1:99268/1‑250 (MQ=255)
                                                                                                                                   tcagccaatctGACGGGCAAAGGTATCGGCGAAGTTTGGCAAGCCTGGCGATTTGTCCGGGGAGATGATTTGCGCTTCTACACGCTCGCCATAATGGGCGAAGAAGGCATTGGGTATGGCCTCGCAAACCTGCTCATGGGTGATCACCGGCAGCAGTTCGGTGAGGTTGGTCACGCGTGAACGCACTG‑CGTTAATGCCTTTCGGCGCCAGTTTCTTTTTATCCGGATTGAGTTAGTTTGCAAggcggcga                                                                                                           >  2:77052/9‑248 (MQ=255)
                                                                                                                                                             cGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCg                                                                                >  1:94662/1‑251 (MQ=255)
                                                                                                                                                               gCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTCAGGTCGGCATTGAGCAGCAAGGTGCCgtg                                                                              >  1:55998/1‑251 (MQ=255)
                                                                                                                                                                        ggCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCtttt                                                                                                                                           <  1:200972/181‑1 (MQ=255)
                                                                                                                                                                                tGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACACGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCtt                                                             >  1:188561/1‑251 (MQ=255)
                                                                                                                                                                                                        ttCCGCTTCTCCCCGCTCGCCATAAGGGGCGAAAAAGGCCTGGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGGGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGACAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGcctatcct                                      <  2:130535/250‑7 (MQ=255)
                                                                                                                                                                                                               tctacacgcTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCttt                                                            >  1:313807/7‑221 (MQ=255)
                                                                                                                                                                                                               tctacacgcTCGCCATAAGGGGCGAAAAAGGCCTTGGTTATGGCCGCGCAAACCTGCTCATGTGTGATCCCCGGCAGCAGCTCGGTTAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCttt                                                            <  2:313807/215‑1 (MQ=255)
                                                                                                                                                                                                                      gcTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCg                       >  1:348907/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ggggCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGGTGGTCACGCGGGAACGCACTGATGT‑AATGCCTTGCGCCGCCAGTTGCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTTAGCAGCAAGGTGCCGTGGTGGAATCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGt            <  1:36970/250‑1 (MQ=255)
                                                                                                                                                                                                                                        aaaaGGCCTTGGTTATGGCCACGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCCCGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGAAAGGTTGCCAGGCGCCTAGGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGGAGCCCCGATCTTTGGATTCGCGATAGGCCGCTCCTGAGACTTAGCGTTCGCCTTCGGCGGgttttatc     >  2:128382/1‑247 (MQ=255)
                                                                                                                                                                                                                                             gCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTGaccacc  >  1:201457/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
AGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACC  >  NC_000913/4623261‑4623746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑