Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,644	G→A	intergenic (+42/‑125)	deoD → / → yjjJ	purine nucleoside phosphorylase/toxin YjjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,644	0	G	A	100.0%	16.9 / NA	8	intergenic (+42/‑125)	deoD/yjjJ	purine nucleoside phosphorylase/toxin YjjJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

ATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621401‑4621695                                                                                                                                                                                                                                                    |                                                    aTACGGCAGCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCGGCAGAGTGTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACt                                              <  2:182377/251‑1 (MQ=255)   aCGGCATCCTCGGCGTGGCAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCTCGAA‑GCCCTGACCATCTGCACCGTGTCTGCCCACATCCGCACTCAAGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATAGCACTGGAATCCGTTCTGCTGTGCGATAAAGAGTAATTGTGATTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGAcggcc                                           >  2:360575/1‑247 (MQ=255)          cctcGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGc                                      <  1:291392/249‑1 (MQ=255)                   ggAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGTCCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTa                             <  1:236685/250‑1 (MQ=255)                                          acggcggtgCTGCAGAGATTGGCTCGAAAGCCCTGGCCATCTGCTCCGTTTCCGACCACATCCGCACTCACGAGCAGACCACTGTCGCTGAGCGTCAGAACAACTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTGCGCTGAAATGCGAACGTCGTTTTAAGCCGGAGCAGGAGACTGCTTCGGCTTTTTAGTAGCCATTCAtcaatagcaactg        <  2:247101/240‑13 (MQ=255)                                              cGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGTCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGACGGAGCAGAAGACTGCTCCGGATTTTTAGTATCTATTCAtcaatagcaactgatttat  >  2:212752/1‑232 (MQ=255)                                                                        cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                >  1:298345/1‑206 (MQ=255)                                                                        cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                <  2:298345/210‑5 (MQ=255)                                                                                                                                                                                                                                                    |                                                    ATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621401‑4621695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑