Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,616	C→A	intergenic (+14/‑153)	deoD → / → yjjJ	purine nucleoside phosphorylase/toxin YjjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,616	0	C	A	100.0%	21.0 / NA	10	intergenic (+14/‑153)	deoD/yjjJ	purine nucleoside phosphorylase/toxin YjjJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (4/6);  total (4/6)

GTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621383‑4621695                                                                                                                                                                                                                                           |                                                                                ggtcaaCGTGATGGAAAAATACGGCATCCCCGGC‑TGTGAAATGGATGCGGCGGGTATCTACGGCGTCGCTGCAGAGGTTGGCGAGAAATCCCTGACCATCGGCACCGTGTCTGACCACATCCGCACTCACGAGCATACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAATTCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGccaacgccgtgt                                                                 <  2:103004/245‑12 (MQ=255) gTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCCCCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCggacgtcgtgtt                                                                >  1:19323/1‑240 (MQ=255)                   aTACGGCAGCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCGGCAGAGTGTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACt                                              <  2:182377/251‑1 (MQ=255)                     aCGGCATCCTCGGCGTG‑GCAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCTCGAA‑GCCCTGACCATCTGCACCGTGTCTGCCCACATCCGCACTCAAGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATAGCACTGGAATCCGTTCTGCTGTGCGATAAAGAGTAATTGTGATTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGAcggcc                                           >  2:360575/1‑247 (MQ=255)                            cctcGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGc                                      <  1:291392/249‑1 (MQ=255)                                     g‑gAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGTCCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTa                             <  1:236685/250‑1 (MQ=255)                                                             acggcggtgCTGCAGAGATTGGCTCGAAAGCCCTGGCCATCTGCTCCGTTTCCGACCACATCCGCACTCACGAGCAGACCACTGTCGCTGAGCGTCAGAACAACTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTGCGCTGAAATGCGAACGTCGTTTTAAGCCGGAGCAGGAGACTGCTTCGGCTTTTTAGTAGCCATTCAtcaatagcaactg        <  2:247101/240‑13 (MQ=255)                                                                 cGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGTCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGACGGAGCAGAAGACTGCTCCGGATTTTTAGTATCTATTCAtcaatagcaactgatttat  >  2:212752/1‑232 (MQ=255)                                                                                           cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                >  1:298345/1‑206 (MQ=255)                                                                                           cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                <  2:298345/210‑5 (MQ=255)                                                                                                                                                                                                                                           |                                                                                GTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621383‑4621695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑