Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,542	T→C	T220T (ACT→ACC)	deoD →	purine nucleoside phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,542	0	T	C	100.0%	45.7 / NA	18	T220T (ACT→ACC)	deoD	purine nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/10);  total (8/10)

GCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621312‑4621695                                                                                                                                                                                                                                         |                                                                                                                                                          gCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCa                                                                                                                                        >  1:50447/1‑250 (MQ=255)            tGATGCTCGCGTGGGAAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTgg                                                                                                                            >  1:298285/1‑251 (MQ=255)                 cgcgggTGGGTACCCTGTTCACCGCTGACCTGTTCTCCTCTCCGGACGGCGAA‑TGGGTTGTCGGGATGGAAAAATCCGGCATCCTCGGCGTG‑GAAATGGAAGCGTCTGGTATTTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGTATca                                                                                                                       <  2:69285/246‑2 (MQ=255)                   cgcgTGGGTATCCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATcc                                                                                                                       <  1:267410/249‑1 (MQ=255)                           tAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTgg                                                                                                             >  1:63611/1‑251 (MQ=255)                                      cgtcTGACCTGTCCTACTCTCCGGACGGCGAACATGG‑CGACGTGATGGCAAAATACGGCATCCGCGGAGGG‑GAAATGGAAGCGGCTGGTATCTACGGTGTCGCGGCATAGGTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGAACACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTTAGACCACCTTCAACGACGTGATCAAAAGCGCACTGGAATCCTTTCTGCTGGGCGATAAag                                                                                                    <  2:118651/246‑1 (MQ=255)                                                                         ggtcaaCGTGATGGAAAAATACGGCATCCCCGGC‑TGTGAAATGGATGCGGCGGGTATCTACGGCGTCGCTGCAGAGGTTGGCGAGAAATCCCTGACCATCGGCACCGTGTCTGACCACATCCGCACTCACGAGCATACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAATTCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGccaacgccgtgt                                                                 <  2:103004/245‑12 (MQ=255)                                                                         gTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCCCCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCggacgtcgtgtt                                                                >  1:19323/1‑240 (MQ=255)                                                                                           aTACGGCAGCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCGGCAGAGTGTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACt                                              <  2:182377/251‑1 (MQ=255)                                                                                             aCGGCATCCTCGGCGTG‑GCAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCTCGAA‑GCCCTGACCATCTGCACCGTGTCTGCCCACATCCGCACTCAAGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATAGCACTGGAATCCGTTCTGCTGTGCGATAAAGAGTAATTGTGATTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGAcggcc                                           >  2:360575/1‑247 (MQ=255)                                                                                                    cctcGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGc                                      <  1:291392/249‑1 (MQ=255)                                                                                                             g‑gAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGTCCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTa                             <  1:236685/250‑1 (MQ=255)                                                                                                                                     acggcggtgCTGCAGAGATTGGCTCGAAAGCCCTGGCCATCTGCTCCGTTTCCGACCACATCCGCACTCACGAGCAGACCACTGTCGCTGAGCGTCAGAACAACTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTGCGCTGAAATGCGAACGTCGTTTTAAGCCGGAGCAGGAGACTGCTTCGGCTTTTTAGTAGCCATTCAtcaatagcaactg        <  2:247101/240‑13 (MQ=255)                                                                                                                                         cGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGTCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGACGGAGCAGAAGACTGCTCCGGATTTTTAGTATCTATTCAtcaatagcaactgatttat  >  2:212752/1‑232 (MQ=255)                                                                                                                                                                  gCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCg                                                                                     <  1:72889/142‑1 (MQ=255)                                                                                                                                                                  gCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCg                                                                                     >  2:72889/1‑142 (MQ=255)                                                                                                                                                                   cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                <  2:298345/210‑5 (MQ=255)                                                                                                                                                                   cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                >  1:298345/1‑206 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                          GCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621312‑4621695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑