Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,491	A→G	V203V (GTA→GTG)	deoD →	purine nucleoside phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,491	0	A	G	96.0%	72.4 / NA	25	V203V (GTA→GTG)	deoD	purine nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (10/14);  minor base T (0/1);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGACTTCGACATGGTGCGTAACGCAGTAGATGCAGCTAAAGCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621269‑4621695                                                                                                                                                                                                                                 |                                                                                                                                                                                                             cGCTGACGTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAg                                                                                                                                                                                   <  1:287269/250‑1 (MQ=255)      aCTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACt                                                                                                                                                                             <  1:22409/251‑1 (MQ=255)                       aacgcgggagacgcggCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTAAGCGTCAGa                                                                                                                                                             <  1:279701/235‑1 (MQ=255)                                            gCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCa                                                                                                                                        >  1:50447/1‑250 (MQ=255)                                                       tGATGCTCGCGTGGGAAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTgg                                                                                                                            >  1:298285/1‑251 (MQ=255)                                                            cgcgggTGGGTACCCTGTTCACCGCTGACCTGTTCTCCTCTCCGGACGGCGAA‑TGGGTTGTCGGGATGGAAAAATCCGGCATCCTCGGCGTG‑GAAATGGAAGCGTCTGGTATTTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGTATca                                                                                                                       <  2:69285/246‑2 (MQ=255)                                                              cgcgTGGGTATCCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATcc                                                                                                                       <  1:267410/249‑1 (MQ=255)                                                                      tAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTgg                                                                                                             >  1:63611/1‑251 (MQ=255)                                                                                 cgtcTGACCTGTCCTACTCTCCGGACGGCGAACATGG‑CGACGTGATGGCAAAATACGGCATCCGCGGAGGG‑GAAATGGAAGCGGCTGGTATCTACGGTGTCGCGGCATAGGTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGAACACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTTAGACCACCTTCAACGACGTGATCAAAAGCGCACTGGAATCCTTTCTGCTGGGCGATAAag                                                                                                    <  2:118651/246‑1 (MQ=255)                                                                                       aCCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATCCGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGTCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCAc                                                                                                                                                                                         <  2:82965/158‑1 (MQ=255)                                                                                       aCCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCAc                                                                                                                                                                                         >  1:82965/1‑158 (MQ=255)                                                                                                                    ggtcaaCGTGATGGAAAAATACGGCATCCCCGGC‑TGTGAAATGGATGCGGCGGGTATCTACGGCGTCGCTGCAGAGGTTGGCGAGAAATCCCTGACCATCGGCACCGTGTCTGACCACATCCGCACTCACGAGCATACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAATTCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGccaacgccgtgt                                                                 <  2:103004/245‑12 (MQ=255)                                                                                                                    gTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCCCCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCggacgtcgtgtt                                                                >  1:19323/1‑240 (MQ=255)                                                                                                                                   aaaaTACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGAc                                                                                                                                                                                                        >  1:342153/1‑100 (MQ=255)                                                                                                                                   aaaaTACGGCATCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGAc                                                                                                                                                                                                        <  2:342153/100‑1 (MQ=255)                                                                                                                                      aTACGGCAGCCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCGGCAGAGTGTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACt                                              <  2:182377/251‑1 (MQ=255)                                                                                                                                        aCGGCATCCTCGGCGTG‑GCAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCTCGAA‑GCCCTGACCATCTGCACCGTGTCTGCCCACATCCGCACTCAAGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATAGCACTGGAATCCGTTCTGCTGTGCGATAAAGAGTAATTGTGATTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGAcggcc                                           >  2:360575/1‑247 (MQ=255)                                                                                                                                               cctcGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGc                                      <  1:291392/249‑1 (MQ=255)                                                                                                                                                        g‑gAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGTCCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTa                             <  1:236685/250‑1 (MQ=255)                                                                                                                                                                                acggcggtgCTGCAGAGATTGGCTCGAAAGCCCTGGCCATCTGCTCCGTTTCCGACCACATCCGCACTCACGAGCAGACCACTGTCGCTGAGCGTCAGAACAACTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTGCGCTGAAATGCGAACGTCGTTTTAAGCCGGAGCAGGAGACTGCTTCGGCTTTTTAGTAGCCATTCAtcaatagcaactg        <  2:247101/240‑13 (MQ=255)                                                                                                                                                                                    cGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGTCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGACGGAGCAGAAGACTGCTCCGGATTTTTAGTATCTATTCAtcaatagcaactgatttat  >  2:212752/1‑232 (MQ=255)                                                                                                                                                                                                             gCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCg                                                                                     <  1:72889/142‑1 (MQ=255)                                                                                                                                                                                                             gCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCg                                                                                     >  2:72889/1‑142 (MQ=255)                                                                                                                                                                                                              cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                >  1:298345/1‑206 (MQ=255)                                                                                                                                                                                                              cccTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACTGCTCCGGCTTTTTAGTATCTATTCAtcaat                <  2:298345/210‑5 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                             CGCTGACTTCGACATGGTGCGTAACGCAGTAGATGCAGCTAAAGCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAA‑ATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTG‑GAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAA  >  NC_000913/4621269‑4621695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑