| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,620,766 | C→T | G388G (GGC→GGT) | deoB → | phosphopentomutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,620,766 | 0 | C | T | 80.8% | 40.4 / 7.4 | 26 | G388G (GGC→GGT) | deoB | phosphopentomutase |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (3/18); total (5/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
TTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCT > NC_000913/4620523‑4620983 | tgccTGTGGCCACCTTCGCGGCGTGATCGGTTATGCTGCGGGGCTGTTGCTGGTCGACCGCAGTCTGCCGGAGTGGATGTCTCTGCTGCGCGATGACGACATCCTGCTCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAct < 2:306294/248‑1 (MQ=255) cggtggctgTTATGCGTCGGGTCTGGTGCTGTTCTACCGCCGGCTGCCGGAGCTGATGTCTCTGTTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACtt < 2:311224/243‑1 (MQ=255) ggatcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCCCCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAAGATTTTGGTACTTCTGATATGGGATATGGCAAAGc < 1:87469/245‑1 (MQ=255) tttCGACCGCCGTCGGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACACCCTGATCCTCACTGCTGACCACTGTTGTGATCCGACCTGGTCCGGTACTGACCTCACTCGTGAACTCATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTc < 1:384064/249‑1 (MQ=255) tcgcacgccgTCTGCCGTATCTGATGTCTCCGCAGCGCGATGACGTTCTCCTGATCCTCACTGCTGTCCACGTTTGTGTTACGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCTGTTCTGGTTTACGGCCCGAATGTAAAACCGGGCTCACTTGGTCACCTTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAACTATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTg < 2:12333/245‑1 (MQ=255) aTGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccgggcccctcc > 1:265939‑M1/1‑213 (MQ=255) aTCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacaggga < 1:41907‑M1/251‑47 (MQ=255) tgcTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGTTATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttttttgccctccccccgcccctccccacccggtggggggggaaaacc > 2:5594‑M1/2‑191 (MQ=255) cACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgata < 1:372486‑M1/251‑68 (MQ=255) gCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGTGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAata > 1:187804/1‑133 (MQ=255) gCGAGCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGAACTGGTATATGGCCCGAAAGTAAACCCGGGCTCACTGTGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAata < 2:187804/133‑1 (MQ=255) gATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAatat < 1:220149/156‑1 (MQ=255) gATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAatat > 2:220149/1‑156 (MQ=255) ggACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggcta < 1:332559‑M1/250‑86 (MQ=255) ggTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccac < 1:4978‑M1/251‑92 (MQ=255) cacGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaac < 1:36646‑M1/226‑78 (MQ=255) ccGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttc < 1:5594‑M1/250‑118 (MQ=255) gTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCttt < 1:151047/160‑1 (MQ=255) gTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCttt > 2:151047/1‑160 (MQ=255) tacggcccGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagtt < 1:159288‑M1/247‑130 (MQ=255) acggccCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagtttt < 1:168401‑M1/249‑132 (MQ=255) gccagaaagTAAAACCGGGCTCACTGGGTCACCGTGAAACCGTCGCGGATAGTGGTCAGACTCTGGTATAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcgccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctgccccactcattaatgcagaaatgggcgatttcgctgacgtagttttgat < 2:365885‑M1/247‑135 (MQ=255) cGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgcc < 1:273144‑M1/251‑138 (MQ=255) cGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacccgct < 1:210379‑M1/251‑150 (MQ=255) tCACTGGGTCCCCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctacc < 2:69239‑M1/184‑88 (MQ=255) aCTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacccgctgcgtgcg < 1:109560‑M1/251‑157 (MQ=255) | TTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCT > NC_000913/4620523‑4620983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |