Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,742	T→C	H380H (CAT→CAC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,742	0	T	C	83.9%	60.4 / 4.3	32	H380H (CAT→CAC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (4/22);  total (7/25)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-02

TCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCT  >  NC_000913/4620498‑4620983                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ccACCAACTGCTTTGATTTCGACCATGCCTGGGGCCCCCGTCGCGACGTCGCCGGTTAGTCTGCGGGTCTTGAGCTGTTCGACCTCCGTCTGCCGGAGCGGAGGTCTCTGCTGCGCGATGACGTCATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACAGTCCGGTACTGGTTTGCGGCCCGAAAGTAAAACCGGGCGCACTTGGTCACCGTg                                                                                                                                                                                                                                               <  2:350041/248‑1 (MQ=255)      cacttcggtgtgttCGGCTCTTCCCGGGGCCACCTACGGGACGTCGCAGGTTATGCTGCTGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACAGCCTGATCCTCACTGCTGTACACGTTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTTACCGTGAAACCtt                                                                                                                                                                                                                                        <  2:55212/239‑1 (MQ=255)       aCTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                                                                                                                                                                                                       <  1:46653/251‑1 (MQ=255)          tcggtgtcttggACTCTGCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCGGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCGGCTGCGCGATGACGACATCCTGATCCTCACTGCGGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                                                                                                                                                                                                       <  2:83880/237‑1 (MQ=255)           cGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGATCGACCGCCGTCTGCCGGAGCTGCTGGCTCTGCTGCGAGATGACGACAGCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACAACCCGCGTGCACACTTTCAGGTACTGGCTGACAGCCCGAAAGGAAAACAGGGCTCACTGGGGCAACATGAAACCTACGCgg                                                                                                                                                                                                                                   >  2:194992/1‑251 (MQ=255)              tGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGATACCTTCGCggca                                                                                                                                                                                                                                 >  1:35403/1‑248 (MQ=255)                          tgccTGTGGCCACCTTCGCGGCGTGATCGGTTATGCTGCGGGGCTGTTGCTGGTCGACCGCAGTCTGCCGGAGTGGATGTCTCTGCTGCGCGATGACGACATCCTGCTCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAct                                                                                                                                                                                                                     <  2:306294/248‑1 (MQ=255)                                               cggtggctgTTATGCGTCGGGTCTGGTGCTGTTCTACCGCCGGCTGCCGGAGCTGATGTCTCTGTTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACtt                                                                                                                                                                                               <  2:311224/243‑1 (MQ=255)                                                                       ggatcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCCCCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAAGATTTTGGTACTTCTGATATGGGATATGGCAAAGc                                                                                                                                                                         <  1:87469/245‑1 (MQ=255)                                                                             tttCGACCGCCGTCGGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACACCCTGATCCTCACTGCTGACCACTGTTGTGATCCGACCTGGTCCGGTACTGACCTCACTCGTGAACTCATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTc                                                                                                                                                                  <  1:384064/249‑1 (MQ=255)                                                                               tcgcacgccgTCTGCCGTATCTGATGTCTCCGCAGCGCGATGACGTTCTCCTGATCCTCACTGCTGTCCACGTTTGTGTTACGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCTGTTCTGGTTTACGGCCCGAATGTAAAACCGGGCTCACTTGGTCACCTTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAACTATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTg                                                                                                                                                                <  2:12333/245‑1 (MQ=255)                                                                                                                      aTGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccgggcccctcc                                                                                                                        >  1:265939‑M1/1‑213 (MQ=255)                                                                                                                              aTCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacaggga                                                                                                                <  1:41907‑M1/251‑47 (MQ=255)                                                                                                                                            tgcTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGTTATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttttttgccctccccccgcccctccccacccggtggggggggaaaacc                                                                                                  >  2:5594‑M1/2‑191 (MQ=255)                                                                                                                                                   cACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgata                                                                                           <  1:372486‑M1/251‑68 (MQ=255)                                                                                                                                                          gCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGTGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAata                                                                                                                                                                                                          >  1:187804/1‑133 (MQ=255)                                                                                                                                                          gCGAGCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGAACTGGTATATGGCCCGAAAGTAAACCCGGGCTCACTGTGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAata                                                                                                                                                                                                          <  2:187804/133‑1 (MQ=255)                                                                                                                                                            gATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAatat                                                                                                                                                                                 <  1:220149/156‑1 (MQ=255)                                                                                                                                                            gATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAatat                                                                                                                                                                                 >  2:220149/1‑156 (MQ=255)                                                                                                                                                                      ggACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggcta                                                                         <  1:332559‑M1/250‑86 (MQ=255)                                                                                                                                                                           ggTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccac                                                                   <  1:4978‑M1/251‑92 (MQ=255)                                                                                                                                                                                      cacGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaac                                                                                 <  1:36646‑M1/226‑78 (MQ=255)                                                                                                                                                                                                      ccGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttc                                         <  1:5594‑M1/250‑118 (MQ=255)                                                                                                                                                                                                         gTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCttt                                                                                                                                >  2:151047/1‑160 (MQ=255)                                                                                                                                                                                                         gTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCttt                                                                                                                                <  1:151047/160‑1 (MQ=255)                                                                                                                                                                                                                  tacggcccGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagtt                             <  1:159288‑M1/247‑130 (MQ=255)                                                                                                                                                                                                                   acggccCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagtttt                           <  1:168401‑M1/249‑132 (MQ=255)                                                                                                                                                                                                                      gccagaaagTAAAACCGGGCTCACTGGGTCACCGTGAAACCGTCGCGGATAGTGGTCAGACTCTGGTATAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcgccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctgccccactcattaatgcagaaatgggcgatttcgctgacgtagttttgat                        <  2:365885‑M1/247‑135 (MQ=255)                                                                                                                                                                                                                         cGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgcc                     <  1:273144‑M1/251‑138 (MQ=255)                                                                                                                                                                                                                                     cGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacccgct         <  1:210379‑M1/251‑150 (MQ=255)                                                                                                                                                                                                                                          tCACTGGGTCCCCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctacc                                                                       <  2:69239‑M1/184‑88 (MQ=255)                                                                                                                                                                                                                                            aCTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacccgctgcgtgcg  <  1:109560‑M1/251‑157 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  TCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCT  >  NC_000913/4620498‑4620983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑